Parametric optimisation of Aspergillus terreus lipase production and its potential in ester synthesis

Gulati, Ruchi; Saxena, Rajendra Kumar; Gupta, Rani; Yadav, Raman P; Davidson, William S.

doi:10.1016/s0032-9592(99)00090-4

Cited by 63 publications

(42 citation statements)

References 14 publications

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“…Co. Inc., Bio-flow IV fermentor). 10,11 The medium was autoclaved at 121 °C (15 psi) for 15 min. and inoculated with 1x10 7 spores/50 ml of the medium using 96 h old culture of A. terreus.…”

Section: Methodsmentioning

confidence: 99%

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Novel biocatalytic esterification reactions on fatty acids: synthesis of sorbitol 1(6) - monostearate

Gulati¹,

Arya²,

Malhotra³

et al. 2003

Arkivoc

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Dedicated to Professor Sukh Dev on his 80th birthday (received 16 Jan 03; accepted 06 May 03; published on the web 08 May 03) AbstractAspergillus terreus lipase has exhibited novel capability of catalyzing esterification reaction between fatty acids (C 4 -C 18 ) and primary, secondary and tertiary monohydric alcohols. Although, the lipase efficiently catalyzed the esterification of saturated stearic acid (C 18:0 ), it failed to accept the monounsaturated oleic acid (C 18:1 ) as the substrate which is also a C 18 acid, but has a double bond. Thus, this enzyme has a great potential to be used as a selective catalyst for the separation of almost identical saturated and unsaturated fatty acids in their mixtures. Extending this work, highly efficient and regioselective conversion of sorbitol to its 1(6) -monostearate has been achieved by Aspergillus terreus lipase -mediated esterification in n-hexane. Ninety four per cent conversion to the ester was achieved in 12 h on optimizing four physico-chemical factors, i.e. molar ratios of substrates, temperature, solvent and water activity. Aspergillus terreus lipase immobilized on Accurel was efficient in the synthesis of sorbitol stearate and was reused three times without any significant decline in its activity, yields were further enhanced to 96 % on using the immobilized enzyme.

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Section: Methodsmentioning

confidence: 99%

“…11 In preliminary investigation, we have observed that the lipase from Aspergillus terreus also catalyses the esterification of fatty acids, e.g. butyric, caproic, caprylic, capric, myristic, palmitic and stearic acids with primary, secondary and tertiary monohydric alcohols.…”

Section: Esterification Of Monohydric Alcoholsmentioning

confidence: 99%

Novel biocatalytic esterification reactions on fatty acids: synthesis of sorbitol 1(6) - monostearate

Gulati¹,

Arya²,

Malhotra³

et al. 2003

Arkivoc

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“…On the contrary, oils, tributyrin, and oleic acid possess an inhibitory effect on lipase production. Gulati et al [36] and Odibo et al [37] demonstrated that an inducer effect was exerted by Triton X-100 in cultures of Hendersonula toruloidea and Aspergillus terreus. The lower results obtained in media containing oils are in agreement with the findings of Nahas [38] and Fadiloglu and Erkmen [39] when studying lipase production by Rhizopus oligosporus and R. oryzae, respectively.…”

Section: Addition Of Lipidic Compoundsmentioning

confidence: 99%

Culture Conditions and Investigation of Bioreactor Configurations for Lipase Production by Rhizopus oryzae

et al. 2010

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Lipolytic enzymes are the subject of great industrial and academic interest. For this reason, a detailed study of lipolytic enzyme production by the fungus Rhizopus oryzae is tackled, and several steps from plate to shake flasks and bioreactor cultures are investigated in order to propose an optimized strategy to perform the biological process. The suitability of several lipidic compounds and surfactants is assessed. Triton X-100 (5 g/L) gives the highest activities with a maximum value of 6320 U/L which is 10-fold the value attained in cultures without addition of lipidic compounds. As there are almost no studies on bench-scale bioreactors, two bioreactor configurations, stirred tank and air-lift, are investigated to determine the most suitable one to carry out the biological reaction. It is demonstrated that the lipolytic activity is strongly enhanced when a stirred-tank bioreactor is used with a maximum value of 3521 U/L within two days which is clearly higher than the values produced by other recently reported species.

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“…Lipases (glycerol ester hydrolases, EC 3.1.1.3) suitable sources for lipase production; since they are catalyze the hydrolysis of fats to produce fatty acids, more stable and can be obtained with much lower costs monoglycerides, diglycerides and glycerol [1,2]. compared to lipases produced from animals and plants [8].…”

Section: Introductionmentioning

confidence: 99%

Lipase Production in Tray-Bioreactor via Solid State Fermentation under Desired Growth Conditions

Vaseghi

Najafpour

Mohseni³

et al. 2012

IJEE

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Lipase was produced under desired growth conditions in a novel tray bioreactor using the fungus strain of Rhizopus oryzae. Several agricultural residues/products including sugarcane bagasse, wheat bran, corn meal, barely bran and equal mixtures of sugarcane bagasse with agricultural residues were applied as solid substrate. Lipase produced from the pure sugarcane bagasse showed higher activities than other substrates; which resulted enzyme activities of 155.76 and 138.37 U/gds for the top and middle trays respectively. Furthermore, the influence of carbon and nitrogen supplements was investigated. Addition of carbon sources as substrate was found to be ineffective, while lipase activity remarkably increased by supplementation of bagasse with adequate amount of nitrogen source. Among the nitrogen supplements, urea as a suitable nitrogen source was considered; as a result the average lipolytic activity of 229.355 U/gds was achieved. In addition, various concentrations of vegetable oils including canola oil, soybean oil, olive oil and castor oil were applied. The inducing effect of vegetable oil on lipase activity was investigated. Among them, olive oil and canola oil increased lipolytic activity of lipase with an average value of 192.26 and 183.57 U/gds, respectively.

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Parametric optimisation of Aspergillus terreus lipase production and its potential in ester synthesis

Cited by 63 publications

References 14 publications

Novel biocatalytic esterification reactions on fatty acids: synthesis of sorbitol 1(6) - monostearate

Novel biocatalytic esterification reactions on fatty acids: synthesis of sorbitol 1(6) - monostearate

Culture Conditions and Investigation of Bioreactor Configurations for Lipase Production by Rhizopus oryzae

Lipase Production in Tray-Bioreactor via Solid State Fermentation under Desired Growth Conditions

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