Parametric control of fiber morphology and tensile mechanics in scaffolds with high aspect ratio geometry produced via melt electrowriting for musculoskeletal soft tissue engineering

Warren, Paul B.; Davis, Zachary G.; Fisher, Matthew B.

doi:10.1016/j.jmbbm.2019.07.013

Cited by 16 publications

(18 citation statements)

References 33 publications

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“…A custom-built DWNFE system was employed, similar to as previously described (19). Briefly, a desktop, fused deposition 3D printer (Lulzbot Mini, Aleph Objects, USA) was augmented by replacing the tool-head with a custom designed adaptor to hold a 5mL Luer-Loc plastic syringe (Jenson Global, USA) fitted with a blunt metal needle (Jenson Global, USA) ( Fig.…”

Section: Scaffold Fabricationmentioning

confidence: 99%

“…Varying printing parameters, such as the needle gauge, needle-to-collector height, and stage speed affects both the fiber diameter and variability of produced fibers (4,17,19). Brown et al have previously shown that decreasing needle size decreases fiber diameter; while Warren et al previously showed changing the needle height can affect fiber deposition for fabricating PCL melt written scaffolds (17,19). Fuh et al previously tested the impact of needle-to-collector height for chitosan-poly(ethylene oxide) hybrid solution and showed a decrease in fiber diameter with increasing distance (28).…”

Section: Spacingmentioning

confidence: 99%

“…Direct-write, near-field electrospinning (DWNFE) combines the software and multi-axis control of 3D printing with the electrohydrodynamic fiber formation of nearfield electrospinning to create complex 3D scaffolds (17)(18)(19). This approach combines the advantages of the two previous methods allowing for the fabrication of a structure with fibers similar in size to the fibers of collagen (2-20µm) while also meeting the macroscopic requirements needed to replicate musculoskeletal fibrous tissue (17)(18)(19)(20)(21). The DWNFE system has even been shown to create 3D stacked fibrous scaffolds with 90-100nm fiber diameters from polyethylene oxide (22).…”

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confidence: 99%

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Processing variables of direct-write, near-field electrospinning impact size and morphology of gelatin fibers

Davis

Hussain

Fisher

2020

Preprint

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Several biofabrication methods are being investigated to produce scaffolds that can replicate the structure of the extracellular matrix. Direct-write, near-field electrospinning of polymer solutions and melts is one such method which combines fine fiber formation with computer-guided control. Research with such systems has focused primarily on synthetic polymers. To better understand the behavior of biopolymers used for direct-writing, this project investigated changes in fiber morphology, size, and variability caused by varying gelatin and acetic acid concentration, as well as, process parameters such as needle gauge and height, stage speed, and interfiber spacing. Increasing gelatin concentration at a constant acetic acid concentration improved fiber morphology from large, planar structures to small, linear fibers with a median of 2.3 µm. Further varying the acetic acid concentration at a constant gelatin concentration did not alter fiber morphology and diameter throughout the range tested. Varying needle gauge and height further improved the median fiber diameter to below 2 µm and variability of the first and third quartiles to within +/-1 µm of the median for the optimal solution combination of gelatin and acetic acid concentrations. Additional adjustment of stage speed did not impact the fiber morphology or diameter. Repeatable interfiber spacings down to 250 µm were shown to be capable with the system. In summary, this study illustrates the optimization of processing parameters for direct-writing of gelatin to produce fibers on the scale of collagen fibers. This system is thus capable of replicating the fibrous structure of musculoskeletal tissues with biologically relevant materials which will provide a durable platform for the analysis of single cell-fiber interactions to help better understand the impact scaffold materials and dimensions have on cell behavior.

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Section: Scaffold Fabricationmentioning

confidence: 99%

Section: Spacingmentioning

confidence: 99%

mentioning

confidence: 99%

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Processing variables of direct-write, near-field electrospinning impact size and morphology of gelatin fibers

Davis

Hussain

Fisher

2020

Preprint

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“…Depending on parameters, such as extrusion pressure, syringe temperature, and needle size, the achievable fiber diameters typically range in the upper tens to hundreds of microns. 28 Recently, electrohydrodynamic printing and electrowriting, which integrate principles from EFF (fiber formation modality) and 3D printing (computer-aided tool path), have also been investigated. 28,29 Although this approach provides good spatial control over fibers similar in diameter to electrospinning, the process is not efficient in creating scaffolds of clinically relevant thickness.…”

Section: Introductionmentioning

confidence: 99%

“…Depending on parameters, such as extrusion pressure, syringe temperature, and needle size, the achievable fiber diameters typically range in the upper tens to hundreds of microns. 28 …”

Section: Introductionmentioning

confidence: 99%

High-Throughput Manufacture of 3D Fiber Scaffolds for Regenerative Medicine

Shirwaiker

Fisher

Anderson

et al. 2020

Tissue Engineering Part C: Methods

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Engineered scaffolds used to regenerate mammalian tissues should recapitulate the underlying fibrous architecture of native tissue to achieve comparable function. Current fibrous scaffold fabrication processes, such as electrospinning and three-dimensional (3D) printing, possess application-specific advantages, but they are limited either by achievable fiber sizes and pore resolution, processing efficiency, or architectural control in three dimensions. As such, a gap exists in efficiently producing clinically relevant, anatomically sized scaffolds comprising fibers in the 1-100 mm range that are highly organized. This study introduces a new highthroughput, additive fibrous scaffold fabrication process, designated in this study as 3D melt blowing (3DMB). The 3DMB system described in this study is modified from larger nonwovens manufacturing machinery to accommodate the lower volume, high-cost polymers used for tissue engineering and implantable biomedical devices and has a fiber collection component that uses adaptable robotics to create scaffolds with predetermined geometries. The fundamental process principles, system design, and key parameters are described, and two examples of the capabilities to create scaffolds for biomedical engineering applications are demonstrated.

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The Importance of Interfaces in Multi‐Material Biofabricated Tissue Structures

Viola

Piluso

Groll

et al. 2021

Adv Healthcare Materials

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Biofabrication exploits additive manufacturing techniques for creating 3Dstructures with a precise geometry that aim to mimic a physiological cellular environment and to develop the growth of native tissues. The most recent approaches of 3D biofabrication integrate multiple technologies into a single biofabrication platform combining different materials within different length scales to achieve improved construct functionality. However, the importance of interfaces between the different material phases, has not been adequately explored. This is known to determine material's interaction and ultimately mechanical and biological performance of biofabricated parts. In this review, this gap is bridged by critically examining the interface between different material phases in (bio)fabricated structures, with a particular focus on how interfacial interactions can compromise or define the mechanical (and biological) properties of the engineered structures. It is believed that the importance of interfacial properties between the different constituents of a composite material, deserves particular attention in its role in modulating the final characteristics of 3D tissue-like structures.

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Parametric control of fiber morphology and tensile mechanics in scaffolds with high aspect ratio geometry produced via melt electrowriting for musculoskeletal soft tissue engineering

Cited by 16 publications

References 33 publications

Processing variables of direct-write, near-field electrospinning impact size and morphology of gelatin fibers

Processing variables of direct-write, near-field electrospinning impact size and morphology of gelatin fibers

High-Throughput Manufacture of 3D Fiber Scaffolds for Regenerative Medicine

The Importance of Interfaces in Multi‐Material Biofabricated Tissue Structures

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