Parametric analyses of anxiety in zebrafish scototaxis

Maximino, Caio; Brito, Thiago Marques; Colmanetti, Rafael; Pontes, Alvaro Antonio Assis; Castro, Henrique Meira de; Lacerda, Renata Inah Tavares de; Morato, Sílvio; Gouvêia, Amauri

doi:10.1016/j.bbr.2010.01.031

Cited by 164 publications

(95 citation statements)

References 45 publications

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“…When zebrafish were tested repeatedly in the same chamber (three trials with a 24-h interval between each trial), we did not observe significant habituation or sensitization of the choice behavior (Fig. 1D, n = 13; P = 0.64), which was consistent with what has been reported recently (24).…”

Section: Resultssupporting

confidence: 81%

Identification of a brain center whose activity discriminates a choice behavior in zebrafish

Lau

Mathur

Gould

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

181

194

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The ability to make choices and carry out appropriate actions is critical for individual survival and well-being. Choice behaviors, from hard-wired to experience-dependent, have been observed across the animal kingdom. Although differential engagement of sensory neuronal pathways is a known mechanism, neurobiological substrates in the brain that underlie choice making downstream of sensory perception are not well understood. Here, we report a behavioral paradigm in zebrafish in which a half-light/ half-dark visual image evokes an innate choice behavior, light avoidance. Neuronal activity mapping using the immediate early gene c-fos reveals the engagement of distinct brain regions, including the medial zone of the dorsal telencephalic region (Dm) and the dorsal nucleus of the ventral telencephalic area (Vd), the teleost anatomical homologs of the mammalian amygdala and striatum, respectively. In animals that were subjected to the identical sensory stimulus but displayed little or no avoidance, strikingly, the Dm and Vd were not engaged, despite similar levels of activation in the brain nuclei involved in visual processing. Based on these findings and previous connectivity data, we propose a neural circuitry model in which the Dm serves as a brain center, the activity of which predicates this choice behavior in zebrafish.emotion | fear | anxiety | decision-making

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Section: Resultssupporting

confidence: 81%

Identification of a brain center whose activity discriminates a choice behavior in zebrafish

Lau

Mathur

Gould

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

181

194

View full text Add to dashboard Cite

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“…When tested for recovery in normal seawater the OA-exposed group returned to control levels after 12 days. Any potential habituation to the light/dark test was not investigated in this study; however, previous work in zebrafish suggests that habituation does not occur in the light/dark test even after 5 consecutive days of testing [44]. As we administered the light/dark test on a minimal schedule and fish were rarely handled, and the change in dark preference was robust between recovery days 7 and 12, habituation to the test was unlikely to occur.…”

Section: Discussionmentioning

confidence: 93%

CO₂-induced ocean acidification increases anxiety in Rockfish via alteration of GABA_Areceptor functioning

2014

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The average surface pH of the ocean is dropping at a rapid rate due to the dissolution of anthropogenic CO 2 , raising concerns for marine life. Additionally, some coastal areas periodically experience upwelling of CO 2 -enriched water with reduced pH. Previous research has demonstrated ocean acidification (OA)-induced changes in behavioural and sensory systems including olfaction, which is due to altered function of neural gamma-aminobutyric acid type A (GABA A ) receptors. Here, we used a camera-based tracking software system to examine whether OA-dependent changes in GABA A receptors affect anxiety in juvenile Californian rockfish (Sebastes diploproa). Anxiety was estimated using behavioural tests that measure light/dark preference (scototaxis) and proximity to an object. After one week in OA conditions projected for the next century in the California shore (1125 + 100 matm, pH 7.75), anxiety was significantly increased relative to controls (483 + 40 matm CO 2 , pH 8.1). The GABA A -receptor agonist muscimol, but not the antagonist gabazine, caused a significant increase in anxiety consistent with altered Cl 2 flux in OA-exposed fish. OA-exposed fish remained more anxious even after 7 days back in control seawater; however, they resumed their normal behaviour by day 12. These results show that OA could severely alter rockfish behaviour; however, this effect is reversible.

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“…Данный поведенческий тест ос-новывается на инстинкте поиска защиты от незна-комой обстановки погружением на дно, фризингом (обездвиживанием или «примерзанием») и сниже-нием двигательного (и исследовательского) пове-дения. После адаптации (акклиматизации) к новой обстановке двигательная активность увеличивает-ся, уменьшается фризинг и увеличивается число пе-реходов в верхнюю половину аквариума [20,22,28]. Подобное поведение традиционно анализируется в тесте «открытое поле» в исследованиях тревожно-сти у грызунов [1,2,8,10,27].…”

Section: Abstract unclassified

Effect of novelty stress on behavioral responses of Danio rerio and assessment of dose-dependent effects of anxiolytics of benzodiazepine structure with phenazepam as an example

Shabanov

Lebedev

Лебедев

et al. 2017

Rev Clin Pharm Drug Ther

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The effect of benzodiazepine anxiolytic phenazepam in Danio rerio was investigated. Previously, Danio rerio showed the effects of other anxiolytics, dia_zepam and chlordiazepoxide. The analysis of the anxiolytic effect of phenazepam in Danio rerio was carried out for the first time. Methods. A stress test on novelty situation was used: a fish was placed first in a beaker with a dissolved pharmacological substance (or water) and then into a novel tank for 6 min, where the trajectory, the path length, the number of movements to the upper part of the novel tank, the number and time of the pattern of “freezing” for each min of the experiment were measured. Results. In response to the novelty of tank, the fish was shown to react by submerging to the bottom, increasing the freezing, and reducing the number of movements to the upper half of the novel tank. After phenazepam exposure (administration), the fish was not only in the lower, but also in the upper part of the novel tank. The average path length did not change significantly in the range of the doses used. The number and time of the freezing, as well as the time spent in the lower part of the novel tank, 2-fold decreased compared to the control group of animals and showed a dose-dependent effect. The number of movements to the upper part of the novel tank for the experiment increased significantly from 1 in the control to 57 after phenazepam in a dose of 1 mg/l. When analyzing the dynamics of the parameters for each min, it was shown that the time of the fish in the lower part of the novel tank decreased from 3th min of the experiment with the use of phenazepam in a dose of 0.5 mg/l. At the same time, the number of movements of fish to the upper part of the novel tank significantly increased more than 2 times from 3th min of the experiment with the use of phenazepam in a dose of 1 mg/l. Conclusion. The described method to study behavioral responses of Danio rerio on novelty stress is high sensitive in comparison with traditional behavioral methods of studing tranquilizers. The prospect of using Danio rerio as animal model in behavioral pharmacology is significant and does not concede research on rodents.

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Parametric analyses of anxiety in zebrafish scototaxis

Cited by 164 publications

References 45 publications

Identification of a brain center whose activity discriminates a choice behavior in zebrafish

Identification of a brain center whose activity discriminates a choice behavior in zebrafish

CO₂-induced ocean acidification increases anxiety in Rockfish via alteration of GABA_Areceptor functioning

Effect of novelty stress on behavioral responses of Danio rerio and assessment of dose-dependent effects of anxiolytics of benzodiazepine structure with phenazepam as an example

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Parametric analyses of anxiety in zebrafish scototaxis

Cited by 164 publications

References 45 publications

Identification of a brain center whose activity discriminates a choice behavior in zebrafish

Identification of a brain center whose activity discriminates a choice behavior in zebrafish

CO2-induced ocean acidification increases anxiety in Rockfish via alteration of GABAAreceptor functioning

Effect of novelty stress on behavioral responses of Danio rerio and assessment of dose-dependent effects of anxiolytics of benzodiazepine structure with phenazepam as an example

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CO₂-induced ocean acidification increases anxiety in Rockfish via alteration of GABA_Areceptor functioning