Parameters which influence the optimal immobilisation of oxidase type enzymes on methacrylate copolymers as demonstrated for amperometric biosensors

Cinnamic ester of D‐sorbitol for immobilization of mushroom tyrosinase

“…Because tyrosinase is an expensive enzyme, immobilizing it is considered to be worthwhile so that it can be re‐used. Several authors have endeavoured to immobilize tyrosinase on different solid supports10–13 using different immobilization methods 11, 14. Attempts have been made to use immobilized tyrosinase for the commercial production of L ‐dopa,15, 16 for the removal of phenolic compounds from wastewater,17, 18 as biosensors to detect pollutants in environmental samples,19, 20 for the electrochemical determination of hydrogen peroxide,12 or for the analysis of thiol‐containing compounds 21.…”

Preparation of highly reusable biocatalysts by immobilization of lipases on epoxy-functionalized silica for production of biodiesel from canola oil

“…Previously, we developed immobilization of RML on this support for hydrolysis of fish oil [34]. The epoxide group can normally undergo a reaction across a wide range of pH values from acidic to basic but the most efficient ring opening is obtained at the extreme of pH [32]. Three lipases that are well known for biodiesel synthesis, R. miehei, T. lanuginosus and C. antarctica lipase B, were immobilized on epoxy functionalized silica, respectively, by mild shaking of the mixture of enzyme and support in phosphate buffer pH 7.…”

“…Silica gel is an amorphous inorganic polymer displaying siloxane groups (Si O Si) and silanol groups (Si OH) on its surface. Recently, modification of silica gel with inorganic and organic functional groups has been performed [30][31][32]. Surface modification is usually implemented by silanization with an organosilane compound.…”

An Amperometric Biosensor Utilizing a Ferrocene-Mediated Horseradish Peroxidase Reaction for the Determination of Capsaicin (Chili Hotness)

“…The principle reaction between HRP and capsaicin in the presence of H 2 O 2 that was mediated by ferrocene in the biosensor membrane system is given in Scheme 1. The biosensor was fabricated via a single-step procedure by incorporating both ferrocene and horseradish peroxidase in a photocured 2-hydroxyethyl methacrylate membrane [16]. …”