Parameters for antimicrobial photodynamic therapy on periodontal pocket—Randomized clinical trial

Alvarenga, Letícia Heineck; Gomes, Ana Carolina Vimieiro; Carribeiro, Pamela; Godoy-Miranda, Bianca; Noschese, Giovane; Ribeiro, Martha S.; Kato, Ilka Tiemy; Bussadori, Sandra Kalil; Pavani, Christiane; Geraldo, Yhago Gazote Eloy; Silva, Daniela de Fátima Teixeira da; Horliana, Anna Carolina Ratto Tempestini; Wainwright, Mark; Prates, Renato Araújo

doi:10.1016/j.pdpdt.2019.05.035

Cited by 29 publications

(13 citation statements)

References 22 publications

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“…Alvarenga et al, also reported that APDT used as an adjuvant treatment showed effective short-term control of periodontitis infection. These results may have occurred due to the periodontal pockets having a clinical probing depth of 5 to 6 mm, being relatively shallow and thus presenting more oxygen in the subgingival portion (14,(19)(20)(21)(22) APDT appears to be a promising option for reducing the amount of periodontopathogenic microorganisms in combination with conventional therapy.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies showed that SDS with APDT on periodontal bacteria produced a significant bacterial reduction. However, more studies are necessary to elucidate the effect of the SDS component on bacterial inactivation (14). This study aims to compare the action of antimicrobial photodynamic chemotherapy using the photosensitizer in SDS and PBS, quantifying the microbial death of A. actinomycetemcomitans and P. gingivalis.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial photodynamic therapy mediated by methylene blue in surfactant vehicle on periodontopathogens

Tortamano

Anselmo

Kassa

et al. 2020

Photodiagnosis and Photodynamic Therapy

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Background: Periodontal disease (PD) is a chronic inflammatory disease caused by the presence of microbial biofilm. The aim of this study was to evaluate antimicrobial effect of antimicrobial photodynamic therapy (APDT) mediated by methylene blue (MB) in monomer form on A. actinomycetemcomitans and P. gingivalis.Methods: A. actinomycetemcomitans ATCC 29523 and P. gingivalis ATCC 33577 were cultured on anaerobic jars at 37°C for 48 h, and we tested APDT in the presence of 0.25% sodium dodecyl sulfate (SDS) in phosphate-buffered saline (PBS) or in PBS alone. APDT was carried out with 100 µM MB under laser radiation (PhotolaseIII, DMC, Brazil) at ʎ = 660 nm and parameters as following (P = 100 mW; I = 250 mW/cm 2 , and doses of 15, 45 and 75 J/cm 2 ).Results: Following APDT, PBS groups of A. actinomycetemcomitans presented 4 Logs of microbial death after 5 min irradiation. However, there was no bacterial reduction in SDS groups. On the other hand, P. gingivalis was sensitive to APDT in the presence of 0.25% SDS with 2 logs reduction from dark toxicity. Conclusion:The presence of 0.25% SDS can lead to different responses depending on the different microbial species.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antimicrobial photodynamic therapy mediated by methylene blue in surfactant vehicle on periodontopathogens

Tortamano

Anselmo

Kassa

et al. 2020

Photodiagnosis and Photodynamic Therapy

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“…Based on a selection of recent publications in which the authors have reported the parameters and settings used to deliver an effective treatment in sufficient detail to allow anyone to repeat the treatment with any laser or LED device [55,85,93,94,95,96,97], the suggested protocol of PDT with λ 635 nm light source for supportive periodontal treatment is summarized in Table 4.…”

Section: Low-energy Photonic Therapymentioning

confidence: 99%

Photonic Therapy in Periodontal Diseases an Overview with Appraisal of the Literature and Reasoned Treatment Recommendations

Giannelli

Lasagni²,

Bani

2019

IJMS

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Recent reviews and meta-analyses of the literature over the past quarter-century have failed to provide enough evidence to prove or disprove the actual utility of photonic therapy in periodontitis, alone or adjunctive to conventional approaches. This apparent paradox has been explained by the many physical, molecular, biological, anatomical, and technical variables of photonic treatments, which can differ in light-emitting devices (laser or LED), wavelengths, irradiation power and modes, clinical objectives, follow-up times, disease grading, and assessment methods. This multi-faceted, controversial scenario has led practitioners to underestimate the actual potential of photonic therapy in periodontal diseases. In this critical appraisal of the literature, we have briefly summarized the main photonic therapies and instruments used in Periodontology, highlighting their main characteristics and limitations. Then, we have tried to identify and discuss the key methodological issues which can have an impact on the outcome of photonic therapies. Our main goal was to identify the best parameters, settings, and methodologies to perform effective periodontal photonic treatments and to extrapolate some recommendations for clinical use. Should these recommendations find a consensus among periodontologists and be adopted in future clinical studies, they will hopefully contribute to dissipate the present confusion and uncertainty on this complex matter.

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“…These biological communities are embedded in polymeric matrixes produced by the cells becoming most of the time recalcitrant to antimicrobial treatment. Microbial biofilms are responsible for most of the infections in humans (2), and aPDT has been successfully used to treat different biofilm-related infections (3)(4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

Photodynamic Activity on Biofilm in Endotracheal Tubes of Patients Admitted to an Intensive Care Unit

Soares

Costa

Miyakawa³

et al. 2020

Photochem & Photobiology

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Ventilator‐associated pneumonia (VAP) is an infection that arises after endotracheal intubation affecting patients under intensive care. The presence of the endotracheal tube (ETT) is a risk factor since it is colonized by multispecies biofilm. Antimicrobial photodynamic therapy (aPDT) could be a strategy to decontaminate ETTs. We verify if methylene blue (MB) associated with external illumination of the ETT could be an alternative to destroy biofilm. We performed an in vitro and ex vivo study. In vitro study was performed with P. aeruginosa biofilm grew over ETT for 7 days. After treatment, the surviving cells were cultured for 3 days and the biofilm was analyzed by crystal violet absorbance. Ex vivo study employed ETT obtained from extubated patients. aPDT was performed with MB (100 µm) and red LED (λ = 640±20 nm). We quantified the biofilm thickness and used scanning electron microscopy and fluorescence technique to verify morphological and functional changes after aPDT. Our results showed that bacteria remain susceptible to aPDT after sequential treatments. We also attested that aPDT can reduce biofilm thickness, disrupt biofilm attachment from ETT surface and kill microbial cells. These data suggest that aPDT should be investigated to decrease VAP incidence via ETT decontamination.

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Parameters for antimicrobial photodynamic therapy on periodontal pocket—Randomized clinical trial

Cited by 29 publications

References 22 publications

Antimicrobial photodynamic therapy mediated by methylene blue in surfactant vehicle on periodontopathogens

Antimicrobial photodynamic therapy mediated by methylene blue in surfactant vehicle on periodontopathogens

Photonic Therapy in Periodontal Diseases an Overview with Appraisal of the Literature and Reasoned Treatment Recommendations

Photodynamic Activity on Biofilm in Endotracheal Tubes of Patients Admitted to an Intensive Care Unit

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