Parameters affecting acetate concentrations during in-situ biological hydrogen methanation

Agneessens, Laura; Ottosen, Lars Ditlev Mørck; Andersen, Martin Hjorth; Olesen, Christina Berg; Feilberg, Anders; Kofoed, Michael Vedel Wegener

doi:10.1016/j.biortech.2018.02.102

Cited by 81 publications

(49 citation statements)

References 35 publications

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“…The high accumulation of acetate in these reactors was likely due to the prevalence of homoacetogens over hydrogenotrophic methanogens as more acetate than methane was produced. Agneessens et al [20] reported that homoacetogens could be more important than hydrogenotrophic methanogens in a low-CO 2 environment due to their higher resistance to high pH.…”

Section: Metabolic Patterns Production During Ex-situ and In-situ Biomentioning

confidence: 99%

“…In contrast, in the work of Bassani et al [19], no hydrolysis inhibition or VFA accumulation was reported after an H 2 addition and pH increase. Agnesseens et al [20] detected a transient accumulation of acetate, which was overcome after several H 2 injections by a microbial adaptation to high H 2 partial pressure. Therefore, each microbial community configuration can adapt differently to the H 2 partial pressure.…”

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confidence: 99%

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Biomethanation processes: new insights on the effect of a high H2 partial pressure on microbial communities

Nan

Trably

Santa-Catalina

et al. 2020

Biotechnol Biofuels

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Background: Biomethanation is a promising solution to upgrade the CH 4 content in biogas. This process consists in the injection of H 2 into an anaerobic digester, using the capacity of indigenous hydrogenotrophic methanogens for converting the injected H 2 and the CO 2 generated from the anaerobic digestion process into CH 4. However, the injection of H 2 could cause process disturbances by impacting the microbial communities of the anaerobic digester. Better understanding on how the indigenous microbial community can adapt to high H 2 partial pressures is therefore required. Results: Seven microbial inocula issued from industrial bioprocesses treating different types of waste were exposed to a high H 2 partial pressure in semi-continuous reactors. After 12 days of operation, even though both CH 4 and volatile fatty acids (VFA) were produced as end products, one of them was the main product. Acetate was the most abundant VFA, representing up to 94% of the total VFA production. VFA accumulation strongly anti-correlated with CH 4 production according to the source of inoculum. Three clusters of inocula were distinguished: (1) inocula leading to CH 4 production, (2) inocula leading to the production of methane and VFA in a low proportion, and (3) inocula leading to the accumulation of mostly VFA, mainly acetate. Interestingly, VFA accumulation was highly correlated to a low proportion of archaea in the inocula, a higher amount of homoacetogens than hydrogenotrophic methanogens and, the absence or the very low abundance in members from the Methanosarcinales order. The best methanogenic performances were obtained when hydrogenotrophic methanogens and Methanosarcina sp. co-dominated all along the operation. Conclusions: New insights on the microbial community response to high H 2 partial pressure are provided in this work. H 2 injection in semi-continuous reactors showed a significant impact on microbial communities and their associated metabolic patterns. Hydrogenotrophic methanogens, Methanobacterium sp. or Methanoculleus sp. were highly selected in the reactors, but the presence of co-dominant Methanosarcinales related species were required to produce higher amounts of CH 4 than VFA.

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Section: Metabolic Patterns Production During Ex-situ and In-situ Biomentioning

confidence: 99%

mentioning

confidence: 99%

Biomethanation processes: new insights on the effect of a high H2 partial pressure on microbial communities

Nan

Trably

Santa-Catalina

et al. 2020

Biotechnol Biofuels

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“…week in samples supplied initially with α-cellulose (SN and SH), which could be due to the rapid decomposition of the organic substrate and sluggish acetoclastic methanogenesis (Agneessens et al, 2018). The situation changed rapidly as the experiment continued and methanogens increased their activity and relative abundances.…”

Section: Biomethane Production Hydrogen Utilization and Analytical mentioning

confidence: 99%

Microbial Community Rearrangements in Power-to-Biomethane Reactors Employing Mesophilic Biogas Digestate

et al. 2019

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The biological conversion of hydrogen (H 2) and carbon dioxide (CO 2) to methane (CH 4), is accomplished by the hydrogenotrophic methanogens (HM). HMs are difficult to cultivate in pure culture, but they are readily available in the mixed culture of effluents from the anaerobic degradation of organic matter, i.e., the fermentation effluent of biogas plants. The rate-limiting step in the work of CH 4-forming microbial communities is the low solubility of H 2 in the aqueous environment. In our approach, the simple fed-batch fermentation technique was selected to supply the gaseous substrates for the microbial community at laboratory scale and mesophilic temperature. Periodically withdrawn samples were analyzed for process parameters and the microbial communities were studied using Terminal Restriction Fragment Length Polymorphism (T-RFLP) of the mcrA gene and Ion Torrent whole metagenome DNA sequencing. The metagenome data were evaluated by both read-based and genome-centric bioinformatics tools. The rearrangements in the mixed microbial communities, triggered by switching the operating conditions to biological power-to-biomethane (bio-P2M), have been established. The production rates were 6.30 mL CH 4 L −1 h −1 during the acclimation phase and 9.21 mL CH 4 L −1 h −1 by the fully adapted community, respectively. The diversity of the anaerobic microbiota decreased as the bio-P2M process progressed. Feeding the community with H 2 apparently promoted the abundance of several genera, in particular Candidatus Cloacimonas and Herbinix. The diversity of the Archaea community decreased considerably upon daily feeding with H 2 and CO 2. The predominant Archea genus was Methanobacterium in every reactor, Methanothrix persisted for the first 4 weeks, while the initially less abundant genus Methanoculleus gained advantage during the adaptation to the sustained bio-P2M process. The accumulation of acetate indicated a strong involvement of homoacetogenic bacteria.

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“…PTR‐MS was used to verify the labeling ratio by measuring acetic acid isotopologues in the headspace of labeled acetate solutions at nominal m/z values of 61 + 43 and 62 + 44, respectively, as described in Agneessens et al and Feilberg et al The natural carbon isotope signatures of CH 4 and CO 2 are expressed using the standard δ notation:

δ^{13} normalC = (() \frac{_{}^{13} C_{italicsample} /_{}^{12} C_{italicsample}}{_{}^{13} C_{italicVPDB} /_{}^{12} C_{italicVPDB}} - 1)

The Vienna Pee Dee Belemnite (VPDB) standard for carbon isotopes is internationally accepted to have a 13 C/ 12 C ratio of 0.011802 . Because δ 13 C values are usually small, they are often expressed in units of ‰ .…”

Section: Methodsmentioning

confidence: 99%

“…The acetic acid concentration in the bulk manure was measured on a gas chromatograph with a flame ionization detector using the sample preparation method described in Mulat and Feilberg. 36 Based on the bulk manure acetic acid concentration, the final 13/12 C atom ratio in acetate (Ac) in the labeled reactors was calculated as described here by assuming a natural 13 37 and Feilberg et al 25 The natural carbon isotope signatures of CH 4 and CO 2 are expressed using the standard δ notation:…”

Section: Estimating Methanogenic Pathwaysmentioning

confidence: 99%

Methanogenic pathways and δ¹³C values from swine manure with a cavity ring‐down spectrometer: Ammonia cross‐interference and carbon isotope labeling

Dalby

Fuchs

Feilberg

2020

Rapid Comm Mass Spectrometry

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Rationale Carbon isotope ratio analysis using cavity ring‐down spectroscopy (CRDS) offers insight into methanogenic activity. In livestock buildings, measuring 13CH4/12CH4 by CRDS is challenging and prone to errors, due to cross‐interference from NH3. Therefore, an interference‐corrected and improved approach to monitor methanogenic pathways with CRDS was developed and described in this study. Methods Cross‐interference from NH3 and other livestock gases on 13CH4/12CH4 was measured using CRDS. The removal efficiency of livestock gases was quantified using proton transfer reaction mass spectrometry. The linearity of the cavity ring‐down spectrometer was tested by dilution of a 13CH4 standard up to 15.6 atom % in CH4. 13C isotope‐labeled acetate was utilized to elucidate the methanogenic pathway contribution in swine manure using a stoichiometric model. Results Significant NH3 cross‐interference on 13CH4/12CH4 was observed, and correction factors were calculated. Cross‐interfering gases were effectively removed using a copper scrubber and a Nafion tube. The instrument responded linearly with 13/12C ratios up to 7.6 atom % in CH4. Isotope‐labeled experiments suggested that hydrogenotrophic methanogenesis was dominant and acetate‐derived CH4 was limited in the studied swine manure. The observations were cross‐validated and consistent with measurements of the natural isotope signatures. Conclusion NH3 cross‐interference leads to major errors in isotope ratios and therefore in evaluating the relative methanogenic pathway activity. We present methodological approaches, which are crucial to apply in livestock‐related uses where NH3 is abundant. This methodology provides a considerable benefit to advance the understanding and reduction of methane emissions in the livestock sector.

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Parameters affecting acetate concentrations during in-situ biological hydrogen methanation

Cited by 81 publications

References 35 publications

Biomethanation processes: new insights on the effect of a high H2 partial pressure on microbial communities

Biomethanation processes: new insights on the effect of a high H2 partial pressure on microbial communities

Microbial Community Rearrangements in Power-to-Biomethane Reactors Employing Mesophilic Biogas Digestate

Methanogenic pathways and δ¹³C values from swine manure with a cavity ring‐down spectrometer: Ammonia cross‐interference and carbon isotope labeling

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Parameters affecting acetate concentrations during in-situ biological hydrogen methanation

Cited by 81 publications

References 35 publications

Biomethanation processes: new insights on the effect of a high H2 partial pressure on microbial communities

Biomethanation processes: new insights on the effect of a high H2 partial pressure on microbial communities

Microbial Community Rearrangements in Power-to-Biomethane Reactors Employing Mesophilic Biogas Digestate

Methanogenic pathways and δ13C values from swine manure with a cavity ring‐down spectrometer: Ammonia cross‐interference and carbon isotope labeling

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Methanogenic pathways and δ¹³C values from swine manure with a cavity ring‐down spectrometer: Ammonia cross‐interference and carbon isotope labeling