Paramagnetic nanofibrous composite films enhance the osteogenic responses of pre-osteoblast cells

Abstract

In this work, a paramagnetic nanofibrous composite film was fabricated with poly lactide, hydroxyapatite and γ-Fe(2)0(3) nanoparticles using the electrospinning technique. The composite film significantly enhanced the proliferation, differentiation and ECM secretion of the osteoblast cells under a static magnetic field, which offers promising application potentials in bone tissue engineering and bone regeneration therapy.

Development and biocompatibility tests of electrospun poly-l-lactide nanofibrous membranes incorporating oleic acid-coated Fe3O4

“…In this study, the applied voltage and polymer concentration were set to 25 kV and 10 wt%, respectively. These parameters are similar to the report of Meng et al [15], who found that beads obviously decrease as the polymer solution concentration increases to 12 wt%. However, these parameters are inconsistent with previous reports [8,24] which found that with an applied voltage of 20 kV, bead-free fibers were achieved only from solutions with polymer concentrations lower than 5 wt%.…”

“…Recently, electrospinning processes have been carried out to develop magnetic fibrous scaffolds containing different kinds of polymers. In these studies, magnetite (Fe 3 O 4 ) has been the most popular magnetic biomaterial, due to its biocompatibility, growth promotion, and stability under various physiological environments [8,15,20,21]. Our results show that the diameters of Fe 3 O 4 nanoparticles are concentrated at 2-8 nm (84.2%) (Figure 2).…”

“…Interestingly, recent studies have demonstrated that magnetic nanoparticles can also improve cellular function, even without magnetic field exposure [14,15]. Tran and Webster [16,17] [2,3,8,18,19].…”

Magnetoactive electrospun nanofibers in tissue engineering applications

“…Meng et al (2010) studied cell proliferation, differentiation and ECM secretion of osteoblast cells in the presence of magnetoactive electrospun nanofibrous composite mats under a static magnetic field, providing a system with promising application potential in bone tissue engineering and bone regeneration treatment [109]. More precisely, nanofibrous scaffolds composed PCL-based magnetoactive electrospun nanofibrous scaffolds were fabricated by J.T.…”

“…In such materials different types of polymers including natural polymers, biopolymers and synthetic polymers, have been combined with magnetic nanoparticles (MNPs) including iron oxide (magnetite) (Fe3O4) [60,[104][105][106], maghemite (γ-Fe2O3) [107][108][109], cobalt (Co) [110], nickel (Ni) [111], iron-platinum (FePt) [112,113] NPs etc. From all the above, iron oxide NPs have been the most widely studied due to their biocompatibility, nontoxicity, and stability and are, by far, the most commonly employed MNPs in biomedical applications [114].…”