Parallel two-step phase-shifting point-diffraction interferometry for microscopy based on a pair of cube beamsplitters

Gao, Peng; Yao, Baoli; Min, Junwei; Guo, Ren; Zheng, Juanjuan; Ye, Tong; Harder, Irina; Nercissian, Vanusch; Mantel, Klaus

doi:10.1364/oe.19.001930

Cited by 66 publications

(26 citation statements)

References 17 publications

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“…Over the past decades, thanks to newly available CCD cameras, high performance of computers, and advances in offline numerical processing techniques, DHM has been fully developed and successfully applied in diverse applications; for instance, in quantitative biology microscopy [2][3][4][5][6][7], surface relief characterization of MEMS or other microstructures [8][9][10][11][12], topography of micro-optics [13][14][15][16], and so on [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Off-axis digital holographic microscopy with LED illumination based on polarization filtering

Guo¹,

Gao²,

Min³

et al. 2013

Appl. Opt.

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A reflection mode digital holographic microscope with light emitting diode (LED) illumination and off-axis interferometry is proposed. The setup is comprised of a Linnik interferometer and a grating-based 4f imaging unit. Both object and reference waves travel coaxially and are split into multiple diffraction orders in the Fourier plane by the grating. The zeroth and first orders are filtered by a polarizing array to select orthogonally polarized object waves and reference waves. Subsequently, the object and reference waves are combined again in the output plane of the 4f system, and then the hologram with uniform contrast over the entire field of view can be acquired with the aid of a polarizer. The one-shot nature in the off-axis configuration enables an interferometric recording time on a millisecond scale. The validity of the proposed setup is illustrated by imaging nanostructured substrates, and the experimental results demonstrate that the phase noise is reduced drastically by an order of 68% when compared to a He-Ne laser-based result.

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Section: Introductionmentioning

confidence: 99%

Off-axis digital holographic microscopy with LED illumination based on polarization filtering

Guo¹,

Gao²,

Min³

et al. 2013

Appl. Opt.

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“…The 2 ambiguity can be retrieved by means of a phase unwrapping process, so the unwrapped optical phase can be obtained. In order to remove the background phase, the phase retrieval procedure should include a reference phase in step where the background is measured beforehand [9,10]; in other words, the background phase is calculated without the target object.…”

Section: Phase Processing Algorithmmentioning

confidence: 99%

Measurement of red blood cell characteristic using parallel phase shifting interferometry

Flores-Muñoz

Toto-Arellano

Lopez-Ortiz

et al. 2015

Optik

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“…However, off-axis recording is prevented since the coherence length of the SLD is lower than the optical path mismatch between both interferometric beams when considering off-axis configuration at SMIM. 43 As a consequence, quasi on-axis (or slightly offaxis) holographic recording [47][48][49] with temporal phase-shifting algorithm [50][51][52] is adopted to retrieve quantitative phase-resolved information of the sample.…”

Section: Introductionmentioning

confidence: 99%

Spatially multiplexed interferometric microscopy with partially coherent illumination

et al. 2016

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We have recently reported on a simple, low cost, and highly stable way to convert a standard microscope into a holographic one [Opt. Express 22, 14929 (2014)]. The method, named spatially multiplexed interferometric microscopy (SMIM), proposes an off-axis holographic architecture implemented onto a regular (nonholographic) microscope with minimum modifications: the use of coherent illumination and a properly placed and selected one-dimensional diffraction grating. In this contribution, we report on the implementation of partially (temporally reduced) coherent illumination in SMIM as a way to improve quantitative phase imaging. The use of low coherence sources forces the application of phase shifting algorithm instead of off-axis holographic recording to recover the sample’s phase information but improves phase reconstruction due to coherence noise reduction. In addition, a less restrictive field of view limitation (1/2) is implemented in comparison with our previously reported scheme (1/3). The proposed modification is experimentally validated in a regular Olympus BX-60 upright microscope considering a wide range of samples (resolution test, microbeads, swine sperm cells, red blood cells, and prostate cancer cells).

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Parallel two-step phase-shifting point-diffraction interferometry for microscopy based on a pair of cube beamsplitters

Cited by 66 publications

References 17 publications

Off-axis digital holographic microscopy with LED illumination based on polarization filtering

Off-axis digital holographic microscopy with LED illumination based on polarization filtering

Measurement of red blood cell characteristic using parallel phase shifting interferometry

Spatially multiplexed interferometric microscopy with partially coherent illumination

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