Parallel steady state studies on a milliliter scale accelerate fed‐batch bioprocess design for recombinant protein production with <i>Escherichia coli</i>

Schmideder, Andreas; Weuster‐Botz, Dirk

doi:10.1002/btpr.2360

Cited by 18 publications

(16 citation statements)

References 41 publications

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“…For example, miniature chemostat systems have been developed for microbial physiology studies [ 24 , 60 , 61 ]. Recently they have also been applied to optimize induction conditions in parallel fermentations of recombinant protein expressing E. coli [ 62 ]. The miniature parallel chemostat was further advanced to a cascade chemostat system where one reactor was used for biomass production and the second reactor for the induction of recombinant protein expression [ 63 ].…”

Section: Advanced Methods For μ-Dependent Recombinant Product Formmentioning

confidence: 99%

Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms

Peebo

Neubauer

2018

Microorganisms

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Depending on the environmental conditions, cells adapt their metabolism and specific growth rate. Rearrangements occur on many different levels such as macromolecular composition, gene and protein expression, morphology and metabolic flux patterns. As the interplay of these processes also determines the output of a recombinant protein producing system, having control over specific growth rate of the culture is advantageous. Continuous culture methods were developed to grow cells in a constant environment and have been used for decades to study basic microbial physiology in a controlled and reproducible manner. Our review summarizes the uses of continuous cultures in cell physiology studies and process development, with a focus on recombinant protein-producing microorganisms.

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Section: Advanced Methods For μ-Dependent Recombinant Product Formmentioning

confidence: 99%

Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms

Peebo

Neubauer

2018

Microorganisms

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“…DASGIP, Jülich/Germany; INFORS HT, Bottmingen/Switzerland; etc.) [15, 16]. The pH can easily be controlled and the feeding-rate can be altered to any desired profile.…”

Section: Introductionmentioning

confidence: 99%

Systematic evaluation of characteristics of the membrane-based fed-batch shake flask

et al. 2017

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BackgroundThe initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions. For a successful scale-up, the operation mode on screening and production scale must be as close as possible. To enable screening under fed-batch conditions, the membrane-based fed-batch shake flask was developed. It is a shake flask mounted with a central feed reservoir with an integrated rotating membrane tip for a controlled substrate release. Building on the previously provided proof of principle for this tool, this work extends its application by constructive modifications and improved methodology to ensure reproducible performance.ResultsThe previously limited operation window was expanded by a systematic analysis of reservoir set-up variations for cultivations with the fast-growing organism Escherichia coli. Modifying the initial glucose concentration in the reservoir as well as interchanging the built-in membrane, resulted in glucose release rates and oxygen transfer rate levels during the fed-batch phase varying up to a factor of five. The range of utilizable membranes was extended from dialysis membranes to porous microfiltration membranes with the design of an appropriate membrane tip. The alteration of the membrane area, molecular weight cut-off and liquid volume in the reservoir offered additional parameters to fine-tune the duration of the initial batch phase, the oxygen transfer rate level of the fed-batch phase and the duration of feeding. It was shown that a homogeneous composition of the reservoir without a concentration gradient is ensured up to an initial glucose concentration of 750 g/L. Finally, the experimental validity of fed-batch shake flask cultivations was verified with comparable results obtained in a parallel fed-batch cultivation in a laboratory-scale stirred tank reactor.ConclusionsThe membrane-based fed-batch shake flask is a reliable tool for small-scale screening under fed-batch conditions filling the gap between microtiter plates and scaled-down stirred tank reactors. The implemented reservoir system offers various set-up possibilities, which provide a wide range of process settings for diverse biological systems. As a screening tool, it accurately reflects the cultivation conditions in a fed-batch stirred tank reactor and enables a more efficient bioprocess development.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-017-0741-6) contains supplementary material, which is available to authorized users.

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“…Bioprocess design is used in virtually every biological process, from surfactants and recombinant protein production to human stem cell culture [75][76][77].…”

Section: Bioprocess Designmentioning

confidence: 99%

“…Pharmaceutical products require high purity while industrial products require lower purity. Chromatography, two aqueous phases extraction are some popular techniques [76,78].…”

Section: Extracellular Productsmentioning

confidence: 99%

Emerging strategies for the development of food industries

et al. 2019

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Undoubtedly, the food industry is undergoing a dynamic process of transformation in its continual development in order to meet the requirements and solve the great problems represented by a constantly growing global population and food claimant in both quantity and quality. In this sense, it is necessary to evaluate the technological trends and advances that will change the landscape of the food processing industry, highlighting the latest requirements for equipment functionality. In particular, it is crucial to evaluate the influence of sustainable green biotechnology-based technologies to consolidate the food industry of the future, today, and it must be done by analyzing the mega-consumption trends that shape the future of industry, which range from local sourcing to on-the-go food, to an increase in organic foods and clean labels (understanding ingredients on food labels). While these things may seem alien to food manufacturing, they have a considerable influence on the way products are manufactured. This paper reviews in detail the conditions of the food industry, and particularly analyzes the application of emerging technologies in food preservation, extraction of bioactive compounds, bioengineering tools and other biobased strategies for the development of the food industry.

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Parallel steady state studies on a milliliter scale accelerate fed‐batch bioprocess design for recombinant protein production with Escherichia coli

Cited by 18 publications

References 41 publications

Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms

Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms

Systematic evaluation of characteristics of the membrane-based fed-batch shake flask

Emerging strategies for the development of food industries

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