2003
DOI: 10.1016/s1046-5928(03)00141-4
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Parallel purification of three catalytic subunits of the protein serine/threonine phosphatase 2A family (PP2AC, PP4C, and PP6C) and analysis of the interaction of PP2AC with alpha4 protein

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Cited by 62 publications
(67 citation statements)
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“…Although the mammalian homolog of Tap42, a4, has been shown to interact with the catalytic subunits of PP2A family members (PP2Ac, PP4c and PP6c), the effect of a4 on phosphatase activity remains controversial and appears to be substratedependent, as it has been shown to both enhance and inhibit PP2A (Murata et al, 1997;Inui et al, 1998). Moreover, the stability of Tap42 Á phosphatase complexes is dependent on rapamycin-sensitive TOR kinase activity, whereas mammalian target of rapamycin (mTOR)-dependent regulation of the a4 Á phosphatase complex is in question (Murata et al, 1997;Chen et al, 1998;Kloeker et al, 2003). Indeed, the role of a4 Á phosphatase complexes as downstream effectors of mTOR is itself dubious.…”
Section: Introductionmentioning
confidence: 99%
“…Although the mammalian homolog of Tap42, a4, has been shown to interact with the catalytic subunits of PP2A family members (PP2Ac, PP4c and PP6c), the effect of a4 on phosphatase activity remains controversial and appears to be substratedependent, as it has been shown to both enhance and inhibit PP2A (Murata et al, 1997;Inui et al, 1998). Moreover, the stability of Tap42 Á phosphatase complexes is dependent on rapamycin-sensitive TOR kinase activity, whereas mammalian target of rapamycin (mTOR)-dependent regulation of the a4 Á phosphatase complex is in question (Murata et al, 1997;Chen et al, 1998;Kloeker et al, 2003). Indeed, the role of a4 Á phosphatase complexes as downstream effectors of mTOR is itself dubious.…”
Section: Introductionmentioning
confidence: 99%
“…Some results derived from immunoprecipitation using different antibodies suggest that the dimeric (AC) form of PP2A may constitute up to a third of the total PP2A pool [22]. Previous studies on the expression of hepatic PP2A components in the adult rat [23][24][25] have identified both dimeric and heterotrimeric PP2A complexes by using affinity purified antibodies specific for PP2A subunits [26]. Limited data are available on the forms of PP2A in fetal liver [27].…”
Section: Introductionmentioning
confidence: 99%
“…38 The phosphatase activity affecting the phosphorylation state of downstream targets of mTOR appears to be dependent on its association with the a4 protein, 36,37,39 an integral component of the rapamycin-sensitive-signaling pathway. 1 The complex a4/ phosphatase and its dephosphorylating activity on S6K is disrupted in the presence of phosphatase inhibitors, 40,41 whereas the effects of rapamycin are controversial. 35,36,39,41 The decreased cell growth rate and the progressive reduction of cell size accompanying or promoted by S6K1 deactivation that follows the inhibition of the mTOR kinase depress cell proliferation 20,29,30,42,43 (with wide variations among cell types) by a complex mechanism still poorly understood.…”
Section: Introductionmentioning
confidence: 99%
“…1 The complex a4/ phosphatase and its dephosphorylating activity on S6K is disrupted in the presence of phosphatase inhibitors, 40,41 whereas the effects of rapamycin are controversial. 35,36,39,41 The decreased cell growth rate and the progressive reduction of cell size accompanying or promoted by S6K1 deactivation that follows the inhibition of the mTOR kinase depress cell proliferation 20,29,30,42,43 (with wide variations among cell types) by a complex mechanism still poorly understood. Whether these changes in cell growth (and size) affect cell survival by inhibition or promotion of cell death is still controversial and no specific experiments have been addressed to evaluate their proapoptotic or antiapoptotic effects on the different death pathways.…”
Section: Introductionmentioning
confidence: 99%