Parallel Microchannel-Based Measurements of Individual Erythrocyte Areas and Volumes

Gifford, Sean C.; Frank, Michael G.; Derganc, Jure; Gabel, Christopher V.; Austin, Robert H.; Yoshida, Tatsuro; Bitensky, Mark W.

doi:10.1016/s0006-3495(03)74882-6

Cited by 87 publications

(114 citation statements)

References 23 publications

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“…For example, an RBC has a large diameter, 8 m, but is only 2 m thick, so if there is a preferential orientation of the RBC in the gap flow pattern at high speeds there will be a dramatic change in bumping of the RBCs. Another possibility is that the shear fields at high flows can deform an RBC into a sausage shape (15).…”

Section: Device Principlesmentioning

confidence: 99%

Deterministic hydrodynamics: Taking blood apart

Davis

Inglis

Morton

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

545

477

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We show the fractionation of whole blood components and isolation of blood plasma with no dilution by using a continuousflow deterministic array that separates blood components by their hydrodynamic size, independent of their mass. We use the technology we developed of deterministic arrays which separate white blood cells, red blood cells, and platelets from blood plasma at flow velocities of 1,000 m͞sec and volume rates up to 1 l͞min. We verified by flow cytometry that an array using focused injection removed 100% of the lymphocytes and monocytes from the main red blood cell and platelet stream. Using a second design, we demonstrated the separation of blood plasma from the blood cells (white, red, and platelets) with virtually no dilution of the plasma and no cellular contamination of the plasma.

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Section: Device Principlesmentioning

confidence: 99%

Deterministic hydrodynamics: Taking blood apart

Davis

Inglis

Morton

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

545

477

View full text Add to dashboard Cite

show abstract

“…[285][286][287] Bitensky's group 285,286 used an in vitro model of the blood microcirculatory system consisting of a network of PDMS microchannels patterned after the dimensions and architecture of the mammalian microcirculation. The device was cast in PDMS, and the channels were rendered hydrophilic to facilitate flow by immobilizing a PEG silane.…”

Section: Vh Blood Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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“…Although providing a geometrically similar environment to capillaries, silicon and glass channels do not have structural properties, such as elastic modulus, of capillary tissue. Recently, structural information of normal erythrocytes was determined by using a human erythrocyte microchannel analyzer made in a silicone elastomer (24). In the present study, we apply similar microfluidic techniques to the study of malaria-infected erythrocytes.…”

mentioning

confidence: 99%

A microfluidic model for single-cell capillary obstruction by Plasmodium falciparum -infected erythrocytes

Shelby

White

Ganesan

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

394

340

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Severe malaria by Plasmodium falciparum is a potentially fatal disease, frequently unresponsive to even the most aggressive treatments. Host organ failure is associated with acquired rigidity of infected red blood cells and capillary blockage. In vitro techniques have played an important role in modeling cell deformability. Although, historically they have either been applied to bulk cell populations or to measure single physical parameters of individual cells. In this article, we demonstrate the unique abilities and benefits of elastomeric microchannels to characterize complex behaviors of single cells, under flow, in multicellular capillary blockages. Channels of 8-, 6-, 4-, and 2-m widths were readily traversed by the 8 m-wide, highly elastic, uninfected red blood cells, as well as by infected cells in the early ring stages. Trophozoite stages failed to freely traverse 2-to 4-m channels; some that passed through the 4-m channels emerged from constricted space with deformations whose shape-recovery could be observed in real time. In 2-m channels, trophozoites mimicked ''pitting,'' a normal process in the body where spleen beds remove parasites without destroying the red cell. Schizont forms failed to traverse even 6-m channels and rapidly formed a capillary blockage. Interestingly, individual uninfected red blood cells readily squeezed through the blockages formed by immobile schizonts in a 6-m capillary. The last observation can explain the high parasitemia in a growing capillary blockage and the well known benefits of early blood transfusion in severe malaria.

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Parallel Microchannel-Based Measurements of Individual Erythrocyte Areas and Volumes

Cited by 87 publications

References 23 publications

Deterministic hydrodynamics: Taking blood apart

Deterministic hydrodynamics: Taking blood apart

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

A microfluidic model for single-cell capillary obstruction by Plasmodium falciparum -infected erythrocytes

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