Parallel gradients in comprehensive multidimensional liquid chromatography enhance utilization of the separation space and the degree of orthogonality when the separation mechanisms are correlated

Aly, Alshymaa A.; Muller, Magriet; Villiers, André de; Pirok, Bob W.J.; Górecki, Tadeusz

doi:10.1016/j.chroma.2020.461452

Cited by 17 publications

(19 citation statements)

References 41 publications

(40 reference statements)

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“…Further improvement in the methodology can however be realized. While the relatively generic RPLC gradient used in the 2 D in the present work led to acceptable separation of the wine phenolics, further improvements may be obtained by using shifted gradients or parallel gradients, which show particular benefit for partially correlated separation systems such as TRLC×RPLC [69]. Furthermore, improvements in the temperature gradient used for TRLC should prove beneficial.…”

Section: Ms/ms Fragmentsmentioning

confidence: 88%

Comprehensive two-dimensional temperature-responsive × reversed phase liquid chromatography for the analysis of wine phenolics

Wicht

Baert

Muller

et al. 2022

Talanta

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Section: Ms/ms Fragmentsmentioning

confidence: 88%

Comprehensive two-dimensional temperature-responsive × reversed phase liquid chromatography for the analysis of wine phenolics

Wicht

Baert

Muller

et al. 2022

Talanta

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“…Considering either T4 or T8 responses, regression analyses were performed to assess the complementarity between each of the different activation markers used, either in ICS or in WAIM (Figure 4). Thus, a strong correlation between two activation markers (extracellular expression or cytokine production) means that the expression of one marker could be predicted by the other, suggesting poor orthogonality and therefore a limited input of the corresponding combination [22][23][24]. Applying this principle with membrane marker or intracellular cytokine expression resulted in generally lower correlation coefficients in the case of WAIM markers, especially for T8 cells for which an average r value of 0.37 was obtained considering all possible associations between CD154, CD137, and CD107a.…”

Section: Correlation Between Elispot Ics and Waim Reactivitymentioning

confidence: 99%

Studying antigen‐specific T cells through a streamlined, whole blood‐based extracellular approach

Trauet,

Bourgoin,

Schuldt

et al. 2023

Cytometry Pt A

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Techniques currently used for the study of antigen‐specific T‐cell responses are either poorly informative or require a heavy workload. Consequently, many perspectives associated with the broader study of such approaches remain mostly unexplored in translational research. However, these could benefit many fields including but not limited to infectious diseases, oncology, and vaccination. Herein, the main objective of this work was to develop a standardized flow cytometry‐based approach that would combine ease of use together with a relevant study of antigen‐specific T‐cell responses so that they could be more often included in clinical research. To this extent, a streamlined approach relying on 1/ the use of whole blood instead of peripheral blood mononuclear cells and 2/ solely based on the expression of extracellular activation‐induced markers (AIMs), called whole blood AIM (WAIM), was developed and further compared to more conventional techniques such as enzyme‐linked immunospot (ELISpot) and flow cytometry‐based intracellular cytokine staining (ICS). Based on a cohort of 20 individuals receiving the COVID‐19 mRNA vaccine and focusing on SARS‐CoV‐2 and cytomegalovirus (CMV)‐derived antigen T‐cell‐specific responses, a significant level of correlation between the three techniques was found. Based on the use of whole blood and on the expression of extracellular activation‐induced markers (CD154, CD137, and CD107a), the WAIM technique appears to be very simple to implement and yet allows interesting patient stratification capabilities as the chosen combination of extracellular markers exhibited higher orthogonality than cytokines that are commonly considered in ICS (IFN‐γ, TNF‐α, and IL‐2).

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“…The two separation methods studied were somewhat correlated, with both being RPC. However, a fractional surface coverage of retention space of roughly 0.73 was exhibited, indicating a decent degree of orthogonality [43].…”

Section: Future Directionsmentioning

confidence: 99%

Recent forensic applications of enhanced chromatographic separation methods

Young

Lurie

2021

J of Separation Science

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This study reviews the recent applications of enhanced separation methods employed in forensic analysis utilizing gas chromatography, liquid chromatography, and supercritical fluid chromatography published between 2015 to 2020, except papers previously covered in relevant review articles. Applications of enhanced chromatographic separation methods to arson investigations, environmental forensics, sexual assault investigations, drug analysis, and toxicology are discussed. Future directions for enhanced chromatographic separation methods in forensic science are also explored.

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Parallel gradients in comprehensive multidimensional liquid chromatography enhance utilization of the separation space and the degree of orthogonality when the separation mechanisms are correlated

Abstract: Parallel gradients in comprehensive multidimensional liquid chromatography enhance utilization of the separation space and the degree of orthogonality when the separation mechanisms are correlated.

Cited by 17 publications

References 41 publications

Comprehensive two-dimensional temperature-responsive × reversed phase liquid chromatography for the analysis of wine phenolics

Comprehensive two-dimensional temperature-responsive × reversed phase liquid chromatography for the analysis of wine phenolics

Studying antigen‐specific T cells through a streamlined, whole blood‐based extracellular approach

Recent forensic applications of enhanced chromatographic separation methods

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