Parallel generation and detection of superoxide and hydrogen peroxide in a fluidic chip

Krylov, Andrey V.; Adamzig, Holger; Walter, Anke; Löchel, Bernd; Kurth, Elke; Pulz, Otto; Szeponik, J.; Wegerich, Franziska; Lisdat, Fred

doi:10.1016/j.snb.2005.11.062

Cited by 21 publications

(25 citation statements)

References 39 publications

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“…Recently, some attempts were made to immobilize XOD in various bioreactors [25][26][27][28][29] but none of these were dedicated to measure the AOC of food samples. Thus, immobilized in bioreactors packed with sepharose gel [25], silica [26] or controlled-pore glass [27][28][29], XOD was used to detect XA [26][27][28], to measure the enzymatic activity of SOD [28,29] or catalase [29], as well as for AOC evaluation of ascorbic acid [29].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, immobilized in bioreactors packed with sepharose gel [25], silica [26] or controlled-pore glass [27][28][29], XOD was used to detect XA [26][27][28], to measure the enzymatic activity of SOD [28,29] or catalase [29], as well as for AOC evaluation of ascorbic acid [29]. However, the AOC of ascorbic acid was estimated only in respect to superoxide radical [29], neglecting its inherent oxidation by H 2 O 2 [30].…”

Section: Introductionmentioning

confidence: 99%

“…When compared with other FIA system recently proposed for AOC evaluation [29], the first advantage of the new design consists in the fact that the AOX comes into the bioreactor simultaneously with XA. In this way, the AOX will arrive in closer vicinity of O 2 C À radicals and locally generated H 2 O 2 , which will increase the reaction probability and, consequently, the efficiency of the whole analytical system.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Antioxidant Capacity by Using Xanthine Oxidase Bioreactor Coupled with Flow‐through H₂O₂ Amperometric Biosensor

Lates¹,

Marty²,

Popescu³

2010

Electroanalysis

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A new flow system for antioxidant capacity (AOC) estimation, consisting of a bioreactor, containing immobilized xanthine oxidase (XOD), coupled with a H 2 O 2 amperometric biosensor, based on Os-wired horseradish peroxidase, was developed. The H 2 O 2 , resulting from the enzymatic reaction between xanthine (XA) and XOD, was amperometrically monitored at À0.1 V vs. Ag/AgCl/KCl sat , in order to avoid the electrochemical interferences. Two protocols were used to perform the AOC evaluation: "steady-state", when the antioxidant (AOX) was injected in the XA flow, and "transient state", when XA and AOX were simultaneously injected in the carrier flow. The AOC of some commercial beverages were evaluated and compared with those obtained with 2,2-diphenyl-1-picrylhydrazyl radical and Folin-Ciocalteu methods.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of Antioxidant Capacity by Using Xanthine Oxidase Bioreactor Coupled with Flow‐through H₂O₂ Amperometric Biosensor

Lates¹,

Marty²,

Popescu³

2010

Electroanalysis

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“…На примере миоглобина электрокаталитическое детектирование пероксида водорода описывается схемой (2.1) [8] Подобно другим гемопротеинам, ион железа гема цитохрома с способен обратимо восстанавливаться и окисляться на поверхности электрода под действием поляризации. Цитохром с проявляет электрокаталитические свойства по отношению к пероксиду водорода, восстанавливая пероксид [59] и также служит сенсорным элементом датчика на пероксид водорода.…”

Section: H 2 O 2 -сенсорыunclassified

Sensor systems for medical application based on hemoproteins and nanocomposite materials

et al. 2010

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1 Учреждение Российской академии медицинских наук Научно-исследовательский институт биомедицинской химии имени В.Н. Ореховича РАМН, 119121, Москва, ул. Погодинская, д. 10; факс: 8 (499) Достижения в нанобиотехнологии в последние годы позволили сделать большой прогресс в разработке сенсорных систем на основе нанокомпозитных материалов. В обзоре рассмотрены собственные и литературные данные по наносенсорным системам на основе функционально-значимых для медицины гемопротеинов. Особое внимание уделено электрохимическим нанобиосенсорам и принципам функционирования сенсорных систем медицинского назначения на их основе.Ключевые слова: гемопротеины, электрохимические биосенсоры, нанокомпозитные материалы, каталаза, пероксидаза, цитохром Р450, гемоглобин, миоглобин.ВВЕДЕНИЕ. Одной из задач инженерной (технической) нанотехнологии является сопряжение наноразмерных биообъектов с измерительными аналитическими устройствами. Получение гибридных бионеорганических нанокомпозитных материалов служит "мостом" для такого сопряжения.Функциональная значимость в организме гемопротеинов высока. Участвуя в различных процессах метаболизма живых существ, гемопротеины играют важную роль в поддержании жизни на земле. Гемоглобин и миоглобин осуществляют транспорт молекул кислорода. Цитохром с переносит электроны в митохондриальной дыхательной цепи. Цитохром с оксидаза катализирует реакцию терминального окисления в дыхательной цепи с участием молекулярного кислорода; пероксидаза -окисление органических субстратов пероксидом водорода, а каталаза -разложение самой перекиси. Цитохромы Р450 участвуют в гидроксилировании органических молекул. Синтез оксида азота из L-аргинина катализирует NO-синтаза (рисунок). С химической точки зрения едва ли не всё это многообразие функций основано на окислительно-восстановительных свойствах железа гема [1]. Редокс-активность каталитического центра гемопротеинов и широкий круг катализируемых ими реакций делают гемопротеины перспективными в создании электрохимических биосенсоров. Конструирование платформ для иммобилизации ферментов и детектирования сигнала вовлекает передовые нанотехнологии и наноматериалы, включая нановолокна [2], углеродные нанотрубки [3][4][5] , модификацию поверхности датчиков наночастицами золота [6][7][8][9], пленками полимеров [10][11][12][13][14][15], различными полимерными мембранами [16][17][18][19][20] и т.д. 55 * -адресат для переписки

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“…Electrochemical biosensors use two main sources of ROS: OH Å and O ÅÀ 2 . The former can be generated photocatalytically [7] or by Fenton reaction in DNA-based antioxidant sensors [8,9], and the latter is mostly enzymatically [2,10,11] but also chemically [3,12,13] or electrochemically [14] formed for the determination of both superoxide radical and AOC. Sensors based on O ÅÀ 2 commonly rely on the immobilization of cytochrome c, which is reduced by superoxide radical, on gold [2][3][4], carbon [15] or screen printedAu-electrode [16] surfaces, where it is reoxidized.…”

Section: Introductionmentioning

confidence: 99%

Electrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessment

Barroso

de‐los‐Santos‐Álvarez

Lobo-Castañón

et al. 2011

Journal of Electroanalytical Chemistry

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a b s t r a c tThe integrity of DNA purine bases was herein used to evaluate the antioxidant capacity. Unlike other DNA-based antioxidant sensors reported so far, the damaging agent chosen was the O ÅÀ 2 radical enzymatically generated by the xanthine/xanthine oxidase system. An adenine-rich oligonucleotide was adsorbed on carbon paste electrodes and subjected to radical damage in the presence/absence of several antioxidant compounds. As a result, partial damage on DNA was observed. A minor product of the radical oxidation was identified by cyclic voltammetry as a diimine adenine derivative also formed during the electrochemical oxidation of adenine/guanine bases. The protective efficiency of several antioxidant compounds was evaluated after electrochemical oxidation of the remaining unoxidized adenine bases, by measuring the electrocatalytic current of NADH mediated by the adsorbed catalyst species generated. A comparison between O ÅÀ 2 and OH Å radicals as a source of DNA lesions and the scavenging efficiency of various antioxidant compounds against both of them is discussed. Finally, the antioxidant capacity of beverages was evaluated and compared with the results obtained with an optical method.

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Parallel generation and detection of superoxide and hydrogen peroxide in a fluidic chip

Cited by 21 publications

References 39 publications

Determination of Antioxidant Capacity by Using Xanthine Oxidase Bioreactor Coupled with Flow‐through H₂O₂ Amperometric Biosensor

Determination of Antioxidant Capacity by Using Xanthine Oxidase Bioreactor Coupled with Flow‐through H₂O₂ Amperometric Biosensor

Sensor systems for medical application based on hemoproteins and nanocomposite materials

Electrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessment

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Parallel generation and detection of superoxide and hydrogen peroxide in a fluidic chip

Cited by 21 publications

References 39 publications

Determination of Antioxidant Capacity by Using Xanthine Oxidase Bioreactor Coupled with Flow‐through H2O2 Amperometric Biosensor

Determination of Antioxidant Capacity by Using Xanthine Oxidase Bioreactor Coupled with Flow‐through H2O2 Amperometric Biosensor

Sensor systems for medical application based on hemoproteins and nanocomposite materials

Electrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessment

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Product

Resources

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Determination of Antioxidant Capacity by Using Xanthine Oxidase Bioreactor Coupled with Flow‐through H₂O₂ Amperometric Biosensor

Determination of Antioxidant Capacity by Using Xanthine Oxidase Bioreactor Coupled with Flow‐through H₂O₂ Amperometric Biosensor