Parallel comparative studies on the toxic effects of unmodified CdTe quantum dots, gold nanoparticles, and carbon nanodots on live cells as well as green gram sprouts

Song, Young-Chae; Duan, Feng; Shi, Wen; Li, Xiaohua; Ma, Huimin

doi:10.1016/j.talanta.2013.05.022

Cited by 65 publications

(42 citation statements)

References 34 publications

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“…此外, 对植物豆芽的生长抑制实验也证实了这三种纳米材料的上述相对毒性, 且以 CdTe QDs 的毒性为最大(图 S1, 支持信息). 这些结果不仅与我们先前的发现一致 [12] , 而且也与其他研究人员的观察吻合 [11,16,17] . 值得指出的是, 尽管在本实验的短时间内 C-dots 没有显现毒性, 但也不能排除在长时间内产生毒副作用.…”

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“…因此, 鉴于纳米材料、特别是 CdTe QDs 的高毒性, 应尽量避免将其应用于生物活体与药物输送 (相对而言, 这些材料较适用于生物体外的分析检测). 实验参照文献 [12] 操作 Figure 1 Viability changes of HeLa cells after 24 h incubation with CdTe QDs, Au NPs, and C-dots at varied concentrations (the viability of the cells in the absence of the nanomaterial is defined as 100%). The results were the mean ± SD of 5 separate measurements.…”

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“…The results were the mean ± SD of 5 separate measurements. The experiments were made as described previously [12] 图 2 HeLa 细胞经不同浓度(0, 10, 25, 50 μg/mL)的纳米材料(A: CdTe QDs; B: AuNPs; C: C-dots)于 37 ℃孵育 24 h 后得到的微分干涉相差 (DIC)成像图. 标尺, 10 μm.…”

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confidence: 99%

“…标尺, 10 μm. 实验参照文献 [12] 操作 Figure 2 The differential interference contrast (DIC) images of HeLa cells treated at 37 ℃ for 24 h with different nanomaterials (A: CdTe QDs; B: AuNPs; C: C-dots) at various concentrations (0, 10, 25, and 50 μg/mL). Scale bar, 10 μm.…”

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“…Scale bar, 10 μm. The experiments were made as described previously [12] 2.2 纳米材料的非均一性其次, 纳米材料的非均一性主要表现在三个方面:…”

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Some Problems of Nanomaterials in Bioanalytical Applications

Song¹,

Liu²,

Zhang³

et al. 2013

Acta Chim. Sinica

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By taking the three commonly used CdTe quantum dots (CdTe QDs), Au nanoparticles (Au NPs) and carbon nanodots (C-dots) as example, we have demonstrated and summarized some noteworthy problems of nanomaterials in bioanalytical applications. These problems mainly include the toxicity, microscopic heterogeneity and environmental susceptivity of nanomaterials, which had been separately discussed with different depths but unfortunately are often overlooked in practice. In the present work, a parallel comparative investigation was first made on cytotoxicity, which reveals that CdTe QDs can lead to the decrease of cell metabolic activity, cell shrinkage, and even cell death; Au NPs at a higher concentration (30 μg/mL) decrease the metabolic activity of cells to a small extent; C-dots exert no obvious toxicity to live cells, showing good biocompatibility. These studies indicate that the relative toxicity of the three nanomaterials in their bare forms is CdTe QDs＞＞AuNPs＞C-dots, which is further supported by the inhibition experiment on the growth of green gram sprouts. Second, the microscopic heterogeneity of nanomaterials was compared with that of resorufin (a small molecular fluorescent dye) by cell imaging experiments, which clearly shows that the distribution of nanomaterials in cells is much less uniform than that of small molecular probes. Besides, the heterogeneity of both size distribution and surface modification/property of nanomaterials is also unavoidable. Third, the environmental susceptivity of nanomaterials was studied, and the results showed that spectroscopic properties of the above three nanomaterials are all sensitive to the pH change of solution. Moreover, AuNPs are also rather susceptible to the ionic strength of solution. The above three problems should arouse high attention of researchers in some bioanalyses. In addition, the measures that we suggested in the paper may be helpful to solve these problems to some extent.

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“…Scale bar, 10 μm. The experiments were made as described previously [12] 2.2 纳米材料的非均一性其次, 纳米材料的非均一性主要表现在三个方面:…”

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Some Problems of Nanomaterials in Bioanalytical Applications

Song¹,

Liu²,

Zhang³

et al. 2013

Acta Chim. Sinica

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Mode of Transfer, Toxicity and Negative Impacts of Engineered Nanoparticles on Environment, Human and Animal Health

Surendhiran

Cui

Lin

2020

The ELSI Handbook of Nanotechnology

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A Si‐CdTe Composite Quantum Dots Probe with Dual‐Wavelength Emission for Sensitively Monitoring Intracellular H₂O₂

Zhou

Zhao

et al. 2022

Adv Funct Materials

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Hydrogen peroxide is an important reactive oxygen species (ROS) that acts as a significant marker of oxidative stress in certain diseases. Owing to the autofluorescence of biological samples, H 2 O 2 monitoring remains challenging. Here, Si-CdTe quantum dots (QDs) are prepared as novel ratiometric fluorescent probes prepared for sensitive detection of H 2 O 2 and glucose, the low detection limit of H 2 O 2 and glucose is 79 and 140 nm, respectively. In this article, CdTe QDs are combined with Si QDs to reduce the cytotoxicity of CdTe. The survival rate of HeLa cells remains at 80% after incubation with the Si-CdTe QDs at a concentration of 500 µg mL −1 for 24 h. Fluorescence extinguishes based on 488 and 514 nm channels are used as internal references to avoid environmental interference and demonstrate selective H 2 O 2 biosensing. In addition, the probe has an excellent colocalization effect in the lysosome under the 514 nm channel, with a maximum Pearson coefficient of 0.96. The advantages of easy preparation, low toxicity, high resistance to photobleaching, and high specificity of the Si-CdTe QDs probes provide a new and convenient means to monitor and detect changes in the endogenous intracellular H 2 O 2 concentration and to select 514 nm lysosomal colocalization dyes.

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Parallel comparative studies on the toxic effects of unmodified CdTe quantum dots, gold nanoparticles, and carbon nanodots on live cells as well as green gram sprouts

Cited by 65 publications

References 34 publications

Some Problems of Nanomaterials in Bioanalytical Applications

Some Problems of Nanomaterials in Bioanalytical Applications

Mode of Transfer, Toxicity and Negative Impacts of Engineered Nanoparticles on Environment, Human and Animal Health

A Si‐CdTe Composite Quantum Dots Probe with Dual‐Wavelength Emission for Sensitively Monitoring Intracellular H₂O₂

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Parallel comparative studies on the toxic effects of unmodified CdTe quantum dots, gold nanoparticles, and carbon nanodots on live cells as well as green gram sprouts

Cited by 65 publications

References 34 publications

Some Problems of Nanomaterials in Bioanalytical Applications

Some Problems of Nanomaterials in Bioanalytical Applications

Mode of Transfer, Toxicity and Negative Impacts of Engineered Nanoparticles on Environment, Human and Animal Health

A Si‐CdTe Composite Quantum Dots Probe with Dual‐Wavelength Emission for Sensitively Monitoring Intracellular H2O2

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Product

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A Si‐CdTe Composite Quantum Dots Probe with Dual‐Wavelength Emission for Sensitively Monitoring Intracellular H₂O₂