Paracrine regulation of keratinocyte proliferation and differentiation

Werner, Sabine; Smola, Hans

doi:10.1016/s0962-8924(01)01955-9

Cited by 157 publications

(141 citation statements)

References 19 publications

Supporting

Mentioning

131

Contrasting

Unclassified

Order By: Relevance

“…Stressed or dying keratinocytes commonly release IL-1a, a cytokine that stimulates production of keratinocyte mitogens by stromal cells 28,29 . To determine a possible role of such paracrineacting growth factors in the hyperproliferation induced by Aldara treatment, we demonstrated upregulation of IL-1a mRNA levels 24 h after one topical Aldara treatment (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…We found that Aldara also activated the NLRP-1 inflammasome in (human) keratinocytes resulting in caspase-1-dependent production of biologically active IL-1b. In addition, Aldara induces expression of G-CSF production by fibroblasts and the induction of S100A8/A9 28,29 and IL-23 production by dermal myeloid cells, which in turn induces IL-17 secretion by dermal gd-T cells 8 . IL-17 and G-CSF have an impact on keratinocytes resulting in their proliferation, whereas S100A8/A9 and CXCL1 are strong chemoattractants for neutrophils.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Aldara activates TLR7-independent immune defence

et al. 2013

Self Cite

View full text Add to dashboard Cite

Aldara is a cream used for topical treatment of non-melanoma skin cancer, and is thought to act through stimulation of anti-tumour immunity. The active ingredient, imiquimod, has been shown to stimulate toll-like receptor 7. Aldara also induces psoriasis-like lesions when applied to naive murine skin, and as such is used as a mouse model for psoriasis. Here we find that in naive murine skin, Aldara induces inflammation largely independently of toll-like receptor 7. Surprisingly, inflammasome activation, keratinocyte death and interleukin 1 release also occur in response to the vehicle cream in the absence of imiquimod. We show that isostearic acid, a major component of the vehicle, promotes inflammasome activation in cultured keratinocytes, and so may contribute to the observed effects of Aldara on murine skin. Aldara therefore stimulates at least two immune pathways independently, and both imiquimod and vehicle are required for a full inflammatory response. Although it remains to be tested, it is possible that imiquimod-independent effects also contribute to the therapeutic efficacy of Aldara.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Aldara activates TLR7-independent immune defence

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…35) KGF also has been shown to be expressed in the dermis and to regulate epidermal proliferation and differentiation via a paracrine mechanism, stimulating wound healing, and hair growth. 36) In contrast, TGF-β induces apoptosis in keratinocytes against cell death, indicating that TGF-β is involved in apoptosis-driven catagen development. 37) In this study, the shaved back skins of 7-week-old C57BL/ 6 mice were topically treated with RB-SCE for 4 weeks.…”

Section: Discussionmentioning

confidence: 99%

<i>In Vivo</i> Hair Growth-Promoting Effect of Rice Bran Extract Prepared by Supercritical Carbon Dioxide Fluid

Choi

Jeon²,

Moon³

et al. 2014

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

The potential hair growth-promoting activity of rice bran supercritical CO 2 extract (RB-SCE) and major components of RB-SCE, linoleic acid, policosanol, γ-oryzanol, and γ-tocotrienol, were evaluated with the histological morphology and mRNA expression levels of cell growth factors using real-time reverse transcriptase-polymerase chain reaction (PCR) in C57BL/6 mice. RB-SCE showed hair growth-promoting potential to a similar extent as 3% minoxidil, showing that the hair follicles were induced to be in the anagen stage. The numbers of the hair follicles were significantly increased. In addition, mRNA expression levels of vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), and keratinocyte growth factor (KGF) were also significantly increased and that of transforming growth factor-β (TGF-β) decreased in RB-SCE-treated groups. Among the major components of RB-SCE, linoleic acid and γ-oryzanol induced the formation of hair follicles according to examination of histological morphology and mRNA expression levels of cell growth factors. In conclusion, our results demonstrate that RB-SCE, particularly linoleic acid and γ-oryzanol, promotes hair growth and suggests RB-SCE can be applied as hair loss treatment. Key words rice bran supercritical CO 2 extract; hair growth-promoting activity; in vivoAlthough hair loss is not a mortal disorder, it has a great impact on a person's self-respect, mental health, and overall quality of life. Approximately 50-60 hairs are normally lost per day, which does not have a noticeable effect on appearance; however, excess loss (>100) results in baldness. Androgenetic alopecia is the most common type of hair loss, affecting millions of both men and women.1) There are many causes of hair loss in men and women, including diseases, nutritional deficiency, aging, hormone imbalance, and stress. Androgenetic alopecia may occur as early as the teenage years, but typically begin in the later decades of life. 2,3) Hair loss affects at least half of all men by the age of 50 and up to 70% of 70-year-old men. 4)

show abstract

“…It is defined as a clinical entity that can affect the skin, nails, mucous membranes and joints, and it can be classified into several categories by the morphological appearance 1 . The most prominent histological features include epidermal hyperproliferation with abnormal keratinocyte differentiation, acanthosis with elongation of the rete ridges and the infiltration of inflammatory cells into the involved skin 2,3 . Although the precise etiology is largely unknown, it is strongly believed that psoriasis is caused by the combination of multiple factors, including the genetic background and environmental insults.…”

Section: Introductionmentioning

confidence: 99%

A Global Gene Expression Analysis of the Peripheral Blood Mononuclear Cells Reveals the Gene Expression Signature in Psoriasis

et al. 2009

View full text Add to dashboard Cite

Background: Psoriasis is a chronic inflammatory skin disease that affects approximately 1~3% of the general population. Objective: We performed cDNA microarray analysis with using the dendrimer labelling method to investigate the gene expression profile in the peripheral blood mononuclear cells (PBMCs) of psoriatic patients. Methods: The peripheral blood mononuclear cells of 5 patients with psoriasis and 8 control subjects were used in the gene expression analyses of psoriasis. Results: We identified 212 differentially expressed genes that showed at least a two-fold induction and/or reduction in psoriatic patients. Among those, 63 genes, including CD44, CD56 and IL7R, were induced, while 139 genes, including the sphingosine kinase 1 and p16-INK genes, were reduced in the psoriatic patients. Conclusion: We can speculate that these genes may have a role for the pathogenesis of psoriasis via their affecting different cellular functions. Our results suggest a possible mechanism by which activated immune cells migrate from the blood to the skin in psoriatic patients, and we provide novel putative targets for developing drugs to treat psoriasis. (Ann Dermatol 21(3) 237∼242, 2009)

show abstract

Paracrine regulation of keratinocyte proliferation and differentiation

Cited by 157 publications

References 19 publications

Aldara activates TLR7-independent immune defence

Aldara activates TLR7-independent immune defence

<i>In Vivo</i> Hair Growth-Promoting Effect of Rice Bran Extract Prepared by Supercritical Carbon Dioxide Fluid

A Global Gene Expression Analysis of the Peripheral Blood Mononuclear Cells Reveals the Gene Expression Signature in Psoriasis

Contact Info

Product

Resources

About