Paracrine Insulin-Like Growth Factor Signaling Influences Primordial Germ Cell Migration: In Vivo Evidence from the Zebrafish Model

Sang, Xianpeng; Curran, Matthew; Wood, Adrienne

doi:10.1210/en.2008-0534

Cited by 30 publications

(29 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IGF-2a and -IGF-2b amino acid sequences share 65 % identity (data not shown). Both genes are maternally deposited and dynamically expressed during embryonic development (Maures et al, 2002;Eivers et al, 2004;Sang et al, 2008). In addition, we show igf-2b expression in the embryonic shield, in anterior neural tissues and the developing heart ( Supplementary Fig.…”

Section: Two Functional Igf-2 Genes In Zebrafishmentioning

confidence: 65%

“…IGF-2 is more highly expressed than IGF-1 during development, indicating that IGF-2 may be the more important IGF ligand during embryonic development (Sang et al, 2008). Two igf-2 genes are present in the zebrafish genome, igf-2a and igf-2b.…”

Section: Introductionmentioning

confidence: 99%

“…They display restricted patterns of expression early in zebrafish development. In particular, the expression of igf-2a at the shield stage, in the notochord and the anterior region of the embryo points to a potential role in neural development (Maures et al, 2002;Eivers et al, 2004;Sang et al, 2008). Studies in other species have revealed expression of igf-2 in embryonic cardiovascular tissue.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Insulin-like growth factor-2 regulates early neural and cardiovascular system development in zebrafish embryos

Hartnett¹,

Glynn²,

Nolan³

et al. 2010

Int. J. Dev. Biol.

View full text Add to dashboard Cite

The insulin-like growth factor (IGF) family is essential for normal embryonic growth and development and it is highly conserved through vertebrate evolution. However, the roles that the individual members of the IGF family play in embryonic development have not been fully elucidated. This study focuses on the role of IGF-2 in zebrafish embryonic development. Two igf-2 genes, igf-2a and igf-2b, are present in the zebrafish genome. Antisense morpholinos were designed to knock down both igf-2 genes. The neural and cardiovascular defects in IGF-2 morphant embryos were then examined further using wholemount in situ hybridisation, TUNEL analysis and O-dianisidine staining. Knockdown of igf-2a or igf-2b resulted in ventralised embryos with reduced growth, reduced eyes, disrupted brain structures and a disrupted cardiovascular system, with igf-2b playing a more significant role in development. During gastrulation, igf-2a and igf-2b are required for development of anterior neural structures and for regulation of genes critical to dorsal-ventral patterning. As development proceeds, igf-2a and igf-2b play antiapoptotic roles. Gene expression analysis demonstrates that igf-2a and igf-2b play overlapping roles in angiogenesis and cardiac outflow tract development. Igf-2b is specifically required for cardiac valve development and cardiac looping. Injection of a dominant negative IGF-1 receptor led to similar defects in angiogenesis and cardiac valve development, indicating IGF-2 signals through this receptor to regulate cardiovascular development. This is the first study describing two functional igf-2 genes in zebrafish. This work demonstrates that igf-2a and igf-2b are critical to neural and cardiovascular development in zebrafish embryos. The finding that igf-2a and igf2b do not act exclusively in a redundant manner may explain why both genes have been stably maintained in the genome.

show abstract

Section: Two Functional Igf-2 Genes In Zebrafishmentioning

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Insulin-like growth factor-2 regulates early neural and cardiovascular system development in zebrafish embryos

Hartnett¹,

Glynn²,

Nolan³

et al. 2010

Int. J. Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…SLa and SLb could act on growth development to some extent as GH does -through IGF signaling. Recently, IGFs have been found to be expressed in teleosts in a wide range of tissues throughout life, which suggests that the autocrine/paracrine role of this hormone may be of particular physiological importance in bony fish (Schlueter et al 2007, Sang et al 2008, Wang et al 2008. For example, gonad-specific IGF3 was found to be present only in the fish genome (Sang et al 2008), which suggests its potential function in gonad development and N o n -t r a n s g e n i c S L α -t r a n s g e n i c S L β -t r a n s g e n i c G H -t r a n s g e n i c 1·45 a ** P < 0·01 * P < 0·05 a Relative POMC mRNA level N o n -t r a n s g e n i c S L α -t r a n s g e n i c S L β -t r a n s g e n i c G H -t r a n s g e n i c N o n -t r a n s g e n i c S L α -t r a n s g e n i c S L β -t r a n s g e n i c G H -t r a n s g e n i c Different letters indicate significant differences (P!0 .…”

Section: Gh Slb Slamentioning

confidence: 99%

A study of somatolactin actions by ectopic expression in transgenic zebrafish larvae

Wan¹,

Chan²

2010

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

Somatolactin (SL) is a fish-specific hormone that belongs to the prolactin (PRL) and GH family. Recently, two forms of SL, SLa and SLb, have been found in some species, and may have different actions and functions. To investigate the role of SL in fish growth and metabolism, we generated transgenic fish founders with ectopic expression of SLa and SLb to study the physiological functions and actions of these SLs among several marker genes. We fused the cDNAs encoding the precursor SLs in frame to a zebrafish b-actin gene promoter to generate transgenic zebrafish lines that were coinjected with a green fluorescent protein (GFP) driven by the same promoter. The transgenic zebrafish were selected based on GFP expression and confirmed by genomic PCR, Southern blot analysis, and transgene expression. Investigations into the expression of marker genes in larvae on different pathways using real-time PCR have provided a general understanding of the actions of SLs. This study found that the overexpression of SLa and SLb in vivo significantly enhanced the transcription of IGFs, insulin, leptin, sterol regulatory element binding protein 1, and fatty acid synthase, as well as the expression level of vitellogenin and proopiomelanocortin, while causing reduced levels of catalase and glutathione S-transferase in the larvae of transgenic zebrafish.

show abstract

“…IGF2 is more highly expressed than IGF1 during development in rodents, ruminants and humans (Delhanty & Han 1993, Hill & Pell 1998, suggesting that it may be the more important IGF during development (Sang et al 2008). Recently, Hills et al (2012) have described IGF2 as a more effective anti-apoptotic survival factor compared with IGF1 in human placental villous cytotrophoblasts.…”

Section: Introductionmentioning

confidence: 99%

IGF2: an endocrine hormone to improve islet transplant survival

Hughes¹,

Rojas-Canales²,

Drogemuller³

et al. 2014

Journal of Endocrinology

View full text Add to dashboard Cite

In the week following pancreatic islet transplantation, up to 50% of transplanted islets are lost due to apoptotic cell death triggered by hypoxic and pro-inflammatory cytokinemediated cell stress. Thus, therapeutic approaches designed to protect islet cells from apoptosis could significantly improve islet transplant success. IGF2 is an anti-apoptotic endocrine protein that inhibits apoptotic cell death through the mitochondrial (intrinsic pathway) or via antagonising activation of pro-inflammatory cytokine signalling (extrinsic pathway), in doing so IGF2 has emerged as a promising therapeutic molecule to improve islet survival in the immediate post-transplant period. The development of novel biomaterials coated with IGF2 is a promising strategy to achieve this. This review examines the mechanisms mediating islet cell apoptosis in the peri-and post-transplant period and aims to identify the utility of IGF2 to promote islet survival and enhance long-term insulin independence rates within the setting of clinical islet transplantation.

show abstract

Paracrine Insulin-Like Growth Factor Signaling Influences Primordial Germ Cell Migration: In Vivo Evidence from the Zebrafish Model

Cited by 30 publications

References 39 publications

Insulin-like growth factor-2 regulates early neural and cardiovascular system development in zebrafish embryos

Insulin-like growth factor-2 regulates early neural and cardiovascular system development in zebrafish embryos

A study of somatolactin actions by ectopic expression in transgenic zebrafish larvae

IGF2: an endocrine hormone to improve islet transplant survival

Contact Info

Product

Resources

About