Paracrine effects of mesenchymal stem cells enhance vascular regeneration in ischemic murine skin

Schlosser, Stefan; Dennler, Cyrill; Schweizer, Riccardo; Eberli, Daniel; Stein, Jens V; Enzmann, Volker; Giovanoli, Pietro; Erni, Dominique; Plock, Jan A.

doi:10.1016/j.mvr.2012.02.011

Cited by 93 publications

(76 citation statements)

References 40 publications

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“…Similar findings were reported by other investigators (28,29) in their work on mouse skin wounds. Another mechanism by which BMSCs could enhance neovascularisation is through the release of pro-angiogenic factors via paracrine mechanism to recruit endothelial lineage cells into wound area (30)(31)(32).…”

Section: Fig 12mentioning

confidence: 99%

Effect of Bone Marrow Derived Mesenchymal Stem Cells on Healing of Induced Full-Thickness Skin Wounds in Albino Rat

Basiouny¹,

Salama²,

Maadawi³

et al. 2013

Int J Stem Cells

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Section: Fig 12mentioning

confidence: 99%

Effect of Bone Marrow Derived Mesenchymal Stem Cells on Healing of Induced Full-Thickness Skin Wounds in Albino Rat

Basiouny¹,

Salama²,

Maadawi³

et al. 2013

Int J Stem Cells

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“…Kaewkhaw et al found that the source of ADSC seem to be determinant in the SC marker expression, differentiation potential and final neurite growth improvement in vitro using three different fat sources on rats (subcutaneous, perinephric and epididymal). Subcutaneous and perinephric ADSC showed superior neurotrophic potential [144]. However, other groups found no different neurotrophic potential of ADSC from different donor region as well as different ages [145].…”

Section: Neurotropic Effects Of Mscmentioning

confidence: 90%

Mesenchymal and Adipose Stem Cell Strategies for Peripheral Nerve Regeneration

Schweizer

Ravuri

Plock

et al. 2015

The Science of Reconstructive Transplantation

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“…Anesthesia and antidote reversion were achieved using triplemix injections as described previously. 9,10 Microdialysis microprobes (CMA20, 100 kDa; Polyethersylfone; CMA Microdialysis, Stockholm, Sweden) were inserted into the peritumoral tissue and perfused with Ringer/hydroxyethyl starch 6% (Fresenius, Oberdorf, Switzerland) using a microinjection pump (CMA/100; Polyethersylfone). The perfusion rate was set to 1 µl/minute as described previously.…”

Section: Microdialysismentioning

confidence: 99%

“…These cells have multiple functions, including the secretion of soluble factors through which they participate and contribute to immunomodulation, antiapoptosis, angiogenesis, and regeneration. [8][9][10] Because of these functionalities, local and migrating stem cells have been suspected to endorse breast cancer development. 11,12 Suggested by reports, adipose-derived mesenchymal stromal cells, and more in general mesenchymal stem cells, can promote progression and metastasis of breast cancer [13][14][15][16][17] through homing of stem cells to tumors as an evident process.…”

mentioning

confidence: 99%

Human Adipose-Derived Mesenchymal Stromal Cells May Promote Breast Cancer Progression and Metastatic Spread

Kamat

Schweizer

Kaenel

et al. 2015

Plastic and Reconstructive Surgery

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BACKGROUND: Stem cell-enriched fat grafting has been proposed as a potential therapy for reconstructive, restorative, or enhancement-related procedures of the breast. Its role in postoncologic breast reconstruction is still emerging, with concerns about safety. The authors investigated the dose-dependent interaction between human adipose-derived mesenchymal stromal cells (AD-MSCs) and human breast cancer cell (BCC) lines [MDA-MB-231 (MDA) and MCF-7 (MCF)] focusing on tumor microenvironment, tumor growth, and metastatic spread. METHODS: Adipose-derived mesenchymal stromal cell influence on viability and factor expression [regulated on activation, normal T cell expressed and secreted (RANTES), tumor necrosis factor-, and eotaxin) of breast cancer cells was studied in vitro using direct and indirect co-culture systems. Groups were formed according to adipose-derived mesenchymal stromal cell-to-cancer cell number ratio [MDA/MCF only, AD-MSC/(MDA/MCF), and AD-MSC/(MDA/MCF)]. A humanized orthotopic murine cancer model was used to evaluate breast cancer progression and metastasis (n = 10/group). Cells were injected into the mammary pad in different ratios and animals were monitored over 42 days. Microdialysis was performed to analyze RANTES levels in the tumor microenvironment (days 21 and 42). Primary and metastatic tumors were weighed and analyzed for oncogene, growth factor, and metastatic marker expression. RESULTS: MDA cell viability increased from 45.5 percent to 95.5 percent in presence of adipose-derived mesenchymal stromal cells in vitro. In vivo, animals with AD-MSC showed increased mean tumor weight (MDA, p < 0.01; MCF versus controls, p < 0.05) and metastatic occurrence (40 percent in MDA; 30 percent in MCF versus 0 percent in controls). Cytokine analysis revealed switching of MCF tumor phenotype to a more malignant type in the presence of adipose-derived mesenchymal stromal cells. CONCLUSION: Human adipose-derived mesenchymal stromal cells may promote progression and metastatic spread in breast cancer through a switch to a more malignant phenotype with worse prognosis. Methods: Adipose-derived mesenchymal stromal cell influence on viability and factor expression [regulated on activation, normal T cell expressed and secreted (RANTES), tumor necrosis factor-α, and eotaxin) of breast cancer cells was studied in vitro using direct and indirect co-culture systems. Groups were formed according to adipose-derived mesenchymal stromal cell-to-cancer cell number ratio [MDA/MCF only, AD-MSC

show abstract

Paracrine effects of mesenchymal stem cells enhance vascular regeneration in ischemic murine skin

Cited by 93 publications

References 40 publications

Effect of Bone Marrow Derived Mesenchymal Stem Cells on Healing of Induced Full-Thickness Skin Wounds in Albino Rat

Effect of Bone Marrow Derived Mesenchymal Stem Cells on Healing of Induced Full-Thickness Skin Wounds in Albino Rat

Mesenchymal and Adipose Stem Cell Strategies for Peripheral Nerve Regeneration

Human Adipose-Derived Mesenchymal Stromal Cells May Promote Breast Cancer Progression and Metastatic Spread

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