Paracoccidioides brasiliensis

San-Blas, Felipe; San-Blas, Gioconda

doi:10.1007/978-1-4684-4982-2_5

Cited by 32 publications

(26 citation statements)

References 29 publications

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“…A study to determine the nature of the precipitating antibodies concluded that they are of the IgG class (108 Circulating immune complexes in paracoccidioidomycosis patients were first described by Arango et al (9) and later confirmed by Chequer-Bou-Habib et al (68,69 6,1993 to prepare, long shelf life, specific (no cross-reactions), reproducible, sensitive, reliable, and reactive in more than one serological test. Different antigen preparations vary greatly in quality, depending on fungal strain, morphological phase, culture medium, size of inoculum, incubation time, and recovery techniques (242,290). According to their origin, P. brasiliensis antigens have been classified as cell wall-derived, cytoplasmic (intracellular), and culture filtrate (exocellular) antigens (99,250,251,270,290).…”

Section: Microbiologymentioning

confidence: 99%

“…Different antigen preparations vary greatly in quality, depending on fungal strain, morphological phase, culture medium, size of inoculum, incubation time, and recovery techniques (242,290). According to their origin, P. brasiliensis antigens have been classified as cell wall-derived, cytoplasmic (intracellular), and culture filtrate (exocellular) antigens (99,250,251,270,290).…”

Section: Microbiologymentioning

confidence: 99%

“…Therefore, the usefulness of cell wall antigens for skin and serologic testing was limited (290 (23,297).…”

Section: Microbiologymentioning

confidence: 99%

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Paracoccidioidomycosis: an update

1993

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This review summarizes knowledge on various aspects of paracoccidioidomycosis. Mycelial propagules, chlamydospores, and arthroconidia exhibit thermal dimorphism; arthroconidia are infectious in animals and, by electron microscopy, appear well provided for survival. The mycelial-to-yeast-phase transformation requires a strict control of glucan synthesis probably mediated by membrane enzymes. Hormonal influences on the transformation of the fungus (mycelium or conidium to yeast phase) have been demonstrated. Estrogen-binding proteins have been detected in the fungal cytosol, and during the transformation novel proteins are produced as a result of estradiol incorporation. Clinical forms have been better defined on the basis of better experimental models. Emphasis has been placed on the lungs as the portal of entry and on the existence of silent pulmonary infections. A specific Paracoccidioides brasiliensis antigen, the 43-kDa glycoprotein (Gp43), has been identified, characterized, and cloned. This has led to improved reproducibility and specificity of serologic tests. The depression of cell-mediated immune responses has been associated with severe disease in humans and in the experimental host. T-cell subsets in patients' tissues were characterized by means of monoclonal antibodies, and a reduced CD4/CD8 ratio was demonstrated. This has been related to alterations in lymphokine and tumor necrosis factor production, production of antigen-antibody complexes, etc. Amphotericin B has provided effective therapy. Azole derivatives have also improved prognosis and facilitated therapy. Itraconazole is presently the drug of choice, yet incapacitating sequelae (mainly pulmonary fibrosis) still constitute major problems.

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Section: Microbiologymentioning

confidence: 99%

Section: Microbiologymentioning

confidence: 99%

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Paracoccidioidomycosis: an update

1993

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“…Paracoccidioides brasiliensis is one of several medically important fungi exhibiting reversible thermal dimorphism (Kane, 1984;Kobayashi et al, 1985;San-Blas & San-Blas, 1985). The transition from the saprophytic mycelial-form (M) into the parasitic yeast-form (Y), which is thought to be a requisite step in the establishment of primary infection (Restrepo, 1985), can be induced by a temperature shift from 25 "C to 37 "C (San-Blas & San-Blas, 1985).…”

Section: Introductionmentioning

confidence: 99%

Influence of Oestradiol on Protein Expression and Methionine Utilization during Morphogenesis of Paracoccidioides brasiliensis

Clemons

Feldman²,

Stevens³

1989

Microbiology

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The temporal sequence of cytosolic protein expression during phase transition of Paracoccidioides brasiliensis was examined. Electrophoretic analysis of cytosol proteins by onedimensional SDS-PAGE revealed numerous differences between the mycelial and yeast forms as well as alterations induced by 17P-oestradiol. Using either protein staining or fluorography of [ SS]methionine-labelled proteins 30 phase-specific bands were detected, 12 mycelial-associated bands (range 30 to 140 kDa) and 18 yeast-associated bands (range 22 to 127 kDa). In cells undergoing mycelial to yeast transition after a shift from 25 "C to 37 "C, the protein patterns showed a temporal progression toward the yeast profile with the accumulation of yeast bands prior to observable morphogenesis. Five novel protein bands (range 23 to 50 kDa) were detected by silver staining during transition. Treatment of temperature-shifted mycelial cultures with 2.6 x 1 OW7 M-oestradiol altered observed profiles ; 4 of 12 mycelial-associated bands were maintained whereas the appearance of the 5 novel transition bands and 9 of 18 yeast-associated bands was blocked or delayed. Analysis of [35S]methionine-labelled proteins revealed that oestradiol induced label uptake by mycelial cells, blocked the synthesis of a 92 kDa yeastspecific band 72 h into transition, and diminished label incorporation 120 h into transition. In conjunction with these steroid-induced alterations of protein expression, little or no morphological transformation occurred. These results support our hypothesis that, analogous to mammalian steroid receptor action, the functional responses of P. brasiliensis to oestradiol are related to regulation of protein expression, presumably mediated via a specific binding proteinligand complex.

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“…Paracoccidioides spp., apresenta uma estrutura complexa de proteínas, glicoproteínas, polissacarídeos, lipídeos e polipeptídeos que reúnem condições físico-químicas e biológicas para atuarem como antígenos (37,38), os quais são reconhecidos pela primeira linha de defesa composta pelas células da resposta imune inata: monócitos, macrófagos, células dendríticas, neutrófilos, e células natural killer (NK), que desempenham um papel central na resistência (39 Os neutrófilos exercem um importante papel imunoprotetor na fase inicial da defesa. Foi demostrado que a depleção desses em diferentes linhagens genéticas gera um aumento nos níveis de anticorpos, que é dependente do isotipo e pode apresentar um papel imunoprotetor ou imunoregulador (48).…”

Section: Resposta Imune Inataunclassified

Paracoccidioides lutzii: estudo de alguns mecanismos de patogenicidade

Jimenez¹,

Eugenia²

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Acho que aqui vai ser em "Martunhol". Olha vai ser tarefa difícil escrever obrigada para cada um com os nomes, mais como fala em português "vai que vai".Ao Professor Dr. Carlos Pelleschi Taborda, que mais do que professor é um amigo. Que aceitou uma ideia louca de trabalhar de novo com conídios noBrasil. E agora todos sabemos, até você professora Vera, porque não dá certo cultivar os conídios sem as condições adequadas.Profe Carlos! Obrigada por ter me proporcionado um grande crescimento profissional em todos os níveis.A você, Professora Dra. Vera Calich, agradeço de coração a oportunidade de trabalhar no seu laboratório. Obrigada pelos ensinamentos.Aos Professores (as), Gilda del Negro, Gil Benard, Nilton Lincopan, Eduardo Bagagli, Sandro Rogério de Almeida, Kelly Ishida, e à Dra Shikanai obrigada pela acessoria e pelo apoio em vários aspectos deste trabalho. Gracias a los amigos en especial a Julito y Ori por haberme recebido en esta ciudad y brindarme no solo hospedaje sino tambiém su amistad, a Isa y Cami que aunque "lejos" en esta gran ciudad son una parte de mis raíces y da estabilidad e a o meu "marido por conveniencia" Buffoni, obrigada pela paciência.As minhas amigas "professoras de português" Márcia Pinto da Silva e Renata Amélia Bueno, pelas experiências e conhecimentos. Pelas sugestões e pela disposição para me ensinar. Muito obrigada! A Luquete porque nos entendemos muito bem, pelas boas e produtivas discussões científicas, pelos experimentos loucos e bizarros a meia noite e pelas novas e boas brejas, com a parceria da Cris. As secretárias da Pós-Graduação do Departamento de Dermatologia.Em memória da senhora Eli Maria. E obrigada Marcia Pia, pela paciência, e a todas as secretarias da Fac de Med e do ICB.A Valeria da Biblioteca obrigada pela ajuda, agilidade, eficácia e gentileza no atendimento, especialmente nestes últimos dias de stress. E finalmente, porém não menos importante, a todas as pessoas que

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Paracoccidioides brasiliensis

Cited by 32 publications

References 29 publications

Paracoccidioidomycosis: an update

Paracoccidioidomycosis: an update

Influence of Oestradiol on Protein Expression and Methionine Utilization during Morphogenesis of Paracoccidioides brasiliensis

Paracoccidioides lutzii: estudo de alguns mecanismos de patogenicidade

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