Paracoccidioides brasiliensis

Restrepo, Ángela; Tobón, Ángela M.

doi:10.1016/b978-0-443-06839-3.00268-x

Cited by 37 publications

(64 citation statements)

References 60 publications

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“…PCM is a systemic disease that involves mainly the lungs but disseminates to the mucous membranes, skin, lymph nodes and adrenals 9 . Due to the local immunosuppression existing in these glands attributable to their high steroid concentrations, they are more susceptible than other intra-abdominal organs to the hematogenous or lymphatic dissemination of certain microorganisms, fungi among them 8 .…”

Section: Discussionmentioning

confidence: 99%

“…The disease affects mainly the lungs but also the mucous membranes and the skin 9 . Adrenal involvement is also relatively frequent but varies according to the report and the methods employed for its diagnosis; in most cases, such diagnosis is established by indirect means such as demonstrating adrenal hypofunction after ACTH challenge or by measurement of its various metabolites.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Paracoccidioides brasiliensis in adrenal glands biopsies of two patients with paracoccidioidomycosis and adrenal insufficiency

Agudelo

Muñoz

Ramírez

et al. 2009

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe authors report two cases of adrenal insufficiency secondary to infiltration of the adrenal glands by Paracoccidioides brasiliensis. The first patient had been treated for a chronic multifocal form of paracoccidiodomycosis 11 years ago. The diagnosis of the mycosis was done simultaneous with that of the adrenal insufficiency in the second patient. In both patients the diagnosis was done by direct visualization of fungus in adrenal biopsies. They were treated with hormonal supplements and itraconazol by 12 and six months, without relapses during the follow-up period.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of Paracoccidioides brasiliensis in adrenal glands biopsies of two patients with paracoccidioidomycosis and adrenal insufficiency

Agudelo

Muñoz

Ramírez

et al. 2009

Rev. Inst. Med. trop. S. Paulo

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“…Fração de alta massa molecular em isolados clínicos de Paracoccidioides brasiliensis Andréa Longoni Fredrich 1 …”

Section: High Molecular Mass Fraction In Clinical Isolates Of Paracocunclassified

High molecular mass fraction in clinical isolates of Paracoccidioides brasiliensis

Fredrich

Nagashima

Pavanelli

et al. 2010

Rev. Soc. Bras. Med. Trop.

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Introduction: Different serum levels of the IgG/IgE for Paracoccidioides brasiliensis high mass molecular (hMM) fraction (~366kDa) in the acute and chronic forms of the disease have been reported. Considering the nonexistence of hMM fraction investigation involving clinical isolates of P. brasiliensis, the present study aimed to investigate the presence of the hMM fraction (~366kDa) in cell free antigens (CFA) from P. brasiliensis clinical isolates. Methods: CFA from 10 clinical isolates and a reference strain (Pb18) were submitted to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) followed by gel image capturing and densitometer analysis. Additionally, CFA from 20 isolates and Pb18 were analyzed by capture ELISA (cELISA) using polyclonal (polAb) or monoclonal (mAb) antibodies to the hMM fraction. Results: The presence of the hMM component was observed in CFA of all samples analyzed by SDS-PAGE/densitometry and by cELISA. In addition, Pearson's correlation test demonstrated stronger coefficients between hMM fraction levels using pAb and mAb (R = 0.853) in cELISA. Conclusions: The soluble hMM fraction was present in all the P. brasiliensis clinical isolates analyzed and the reference strain Pb18, which could be used as a source of this antigen. The work also introduces for first time, the cELISA method for P. brasiliensis hMM fraction detection. Analysis also suggests that detection is viable using polAb or mAb and this methodology may be useful for future investigation of the soluble hMM fraction (~366kDa) in sera from PCM patients.

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“…Colombia, Venezuela, Ecuador and Bolivia follow in incidence albeit at an important distance (1,2). In paracoccidioidomycosis the primary infection site is represented by he lungs but dissemination to other organs occurs frequently (3).…”

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confidence: 99%

“…Two distinctive clinical forms are found, the chronic and the acute/subacute (4,5), with predominance of adult males as in females the inhibitory action of their hormones hinders the micelium to yeast transition process during the initial stages of the infection (6,7). The disease is caused by Paracoccidioides brasiliensis, a thermally dimorphic fungus that in tissues and cultures at 37 °C appears as round to oval yeast cells of variable size (6 to 40 µm) and in the environment or in vitro at temperatures lower than 24 °C, it changes its morphology to the mold form which is composed of thin, septated hyphae capable to producing asexual propagules, microconidia (<5 µm), when cultured under starvation conditions in minimal media (2). Conidia and mycelial fragments are considered the infectious particles.…”

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confidence: 99%

Análisis de la cinética de expresión de genes durante la transición de micelio a levadura y la germinación levadura a micelio en Paracoccidioides brasiliensis

et al. 2011

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Introduction:Paracoccidioidomycosis is an endemic systemic mycosis caused by Paracoccidioides brasiliensis, a thermally dimorphic fungus that in tissues and cultures at 37 °C grows as a yeast while at lower temperatures (less than 24 °C) it becomes a mold; however the genes that rule these processes and their expression are poorly understood. Objective: This research focused on the kinetic expression of certain genes in P. brasiliensis throughout the dimorphic process, one that involves the transition from the mycelium to yeast forms and the germination from the yeast to mycelium form. Materials and methods:A real-time quantitative polymerase chain reaction (RT-qPCR) was optimized to measure the expression of ten genes connected with diverse cellular functions including cell synthesis and wall structure, oxidative stress response, heat shock response, metabolism, proteins' processing, solute transport across the cell membrane and signal transduction pathways at different time points during the mycelia to yeast transition, as well as in the yeast to mycelia germination processes. Results: Genes involved in cell synthesis and wall structure, metabolism and signal transduction were differentially expressed and highly up-regulated during the yeast to mycelia germination process; on the other hand, genes involved in heat shock response, cell synthesis and wall structure were highly up-regulated during the mycelia to yeast transition process. The remaining genes were differentially regulated during both processes. Conclusion: In this work the up-regulation of certain genes involved in the morphological changes occurring in P. brasiliensis yeast and mycelia forms were confirmed, indicating that these biological processes play an important role during the host-pathogen interactions, as well as in the fungus adaptation to environmental conditions

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Paracoccidioides brasiliensis

Cited by 37 publications

References 60 publications

Identification of Paracoccidioides brasiliensis in adrenal glands biopsies of two patients with paracoccidioidomycosis and adrenal insufficiency

Identification of Paracoccidioides brasiliensis in adrenal glands biopsies of two patients with paracoccidioidomycosis and adrenal insufficiency

High molecular mass fraction in clinical isolates of Paracoccidioides brasiliensis

Análisis de la cinética de expresión de genes durante la transición de micelio a levadura y la germinación levadura a micelio en Paracoccidioides brasiliensis

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