Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput

Garcia, Sabrina; Bernabeu, Pamela Romina; Vio, Santiago Adolfo; Cattelan, Natalia; García, Julia E.; Puente, Mariana; Galar, María Lina; Prieto, Claudio; Luna, María Flavia

doi:10.1007/s11104-019-04174-y

Cited by 13 publications

(22 citation statements)

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“…Also, no amplification of DNA with 16S‐rRNA‐specific primers for P. tropica (Btrop PCR) was observed in any of the macerate root and stem samples of inoculated plants, whether nondisinfected or disinfected, under any of the growth conditions tested. Subsequently, a nested PCR with P. tropica ‐specific primers [17] was run to increase the sensitivity of the methodology [31] to detect P. tropica in samples that had been negative for Btrop PCR. No amplification of DNA with the Nested I primers was detected in tissue samples of inoculated plants (data not shown), but we did identify a PCR product of 184 bp after Nested II PCR for all the inoculated plant samples.…”

Section: Resultsmentioning

confidence: 99%

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Tracking and plant growth‐promoting effect of Paraburkholderia tropica MTo‐293 applied to Solanum lycopersicum

et al. 2022

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Paraburkholderia tropica MTo-293 was applied as an experimental bio-input to Solanum lycopersicum (tomato) cv. Platense. Different plant growth systems and inoculation strategies were tested to evaluate P. tropica plant colonization at the seedling stage (growth chamber) using culture-dependent andindependent techniques. The effect of P. tropica on plant growth was evaluated in the growth chamber and greenhouse (productive stage) by biomass accumulation and fruit production, respectively. P. tropica was able to colonize the surface and inner root and stem of tomato seedlings regardless of the inoculation strategy-at sowing and/or before transplanting-showing the competitive nature of P. tropica in nonsterile substrate systems. A nested polymerase chain reaction was validated to track P. tropica in tomato plants even in the inner stem with endophytic P. tropica populations of less than 10 2 CFU g -1 of fresh weight. Efficient colonization of P. tropica correlated with a positive effect on tomato growth when applied at sowing and/or before transplanting: plant growth promotion was observed not only at the seedling stage but also at productive stages improving crop yield in two different seasons. To our knowledge, this report is the first to track and evaluate the plant growth-promoting effect of P. tropica MTo-293 in tomato plants grown in nonsterile substrate systems.

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Section: Resultsmentioning

confidence: 99%

“…Raw sequences were aligned and a consensus sequence was obtained using Clustal Omega [26]. The identity of the inoculated bacteria was confirmed as P. tropica when the alignment was found to be identical to the corresponding fragment sequence of P. tropica MTo‐293 [17].…”

Section: Methodsmentioning

confidence: 99%

Tracking and plant growth‐promoting effect of Paraburkholderia tropica MTo‐293 applied to Solanum lycopersicum

et al. 2022

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“…Similarly, P. tropica isolates possess the highest number of genes encoding phosphatases, siderophore exporter and indoleacetic acid synthase. The potential of P. tropica to enhance plant growth through these processes have also been documented in few recent studies 14, 18 .…”

Section: Discussionmentioning

confidence: 92%

“…Specifically, this study is the first attempt to examine the synergistic interactions of Medicago sativa L. and Paraburkholderia tropica for enhanced rhizoremediation of diesel fuel-contaminated soils. This is highly relevant considering recent studies have shown that Paraburkholderia strains can potentially promote plant growth and/or degrade contaminants 13, 14 Additionally, our earlier studies revealed that M. sativa has high tolerance to petroleum hydrocarbons, relative to other plant species 15 , thereby making it an ideal plant to investigate plant-microbe synergy for biodegradation. By combining genome studies of P. tropica with a pot-based rhizodegradation experiment, we demonstrate that synergistic interactions between M. sativa and P. tropica promotes rhizodegradation of petroleum hydrocarbons.…”

Section: Introductionmentioning

confidence: 92%

Exploiting synergistic interactions ofMedicago sativaL. andParaburkholderia tropicafor enhanced biodegradation of diesel fuel hydrocarbons

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et al. 2021

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The biotechnological application of microorganisms for rhizoremediation of contaminated sites requires the development of plant-microbe symbionts capable of plant growth promotion and hydrocarbon degradation. Studies focusing on microbial consortia are often difficult to reproduce, thereby necessitating the need for culturable single bacterial species for biotechnological applications. Through genomic analyses and plant growth experiments, we examined the synergistic interactions of Medicago sativa L. and Paraburkholderia tropica for enhanced remediation of diesel fuel-contaminated soils. Comparative genomics revealed strong potential of P. tropica for plant growth-promotion, chemotaxis and motility, root nodulation and colonization, and diesel fuel degradation. Plant growth experiments confirmed that P. tropica thrived in the contaminated soils and effectively enhanced M. sativa growth. Geochemical analysis showed that the M. sativa + P. tropica treatment resulted in an efficient degradation of diesel fuel hydrocarbons within two months, offering great prospects for enhanced biodegradation of organic pollutants.

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“…Paraburkholderia tropica MDIIAzo225 is a nitrogen fixing bacterium isolated from maize rhizosphere (unpublished results ENEA). Strains belonging to this species have been proven to exert PGP on several crops to do their ability to form biofilm and colonize plant-tissues [ 90 , 91 ]. Among the two strains belonging to the Azotobacter , A. vinelandii DSM 2289 was reported to increase the growth, chlorophyll content and iron content of soybean plants grown in calcareous soil [ 40 ], whereas A. chroococcum LS132 is a nitrogen fixing bacterium with the ability to improve the growth of tomato plants (unpublished results, AGRIGES).…”

Section: Resultsmentioning

confidence: 99%

Identification of Beneficial Microbial Consortia and Bioactive Compounds with Potential as Plant Biostimulants for a Sustainable Agriculture

et al. 2021

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A growing body of evidence demonstrates the potential of various microbes to enhance plant productivity in cropping systems although their successful field application may be impaired by several biotic and abiotic constraints. In the present work, we aimed at developing multifunctional synthetic microbial consortia to be used in combination with suitable bioactive compounds for improving crop yield and quality. Plant growth-promoting microorganisms (PGPMs) with different functional attributes were identified by a bottom-up approach. A comprehensive literature survey on PGPMs associated with maize, wheat, potato and tomato, and on commercial formulations, was conducted by examining peer-reviewed scientific publications and results from relevant European projects. Metagenome fragment recruitments on genomes of potential PGPMs represented in databases were also performed to help identify plant growth-promoting (PGP) strains. Following evidence of their ability to coexist, isolated PGPMs were synthetically assembled into three different microbial consortia. Additionally, the effects of bioactive compounds on the growth of individually PGPMs were tested in starvation conditions. The different combination products based on microbial and non-microbial biostimulants (BS) appear worth considering for greenhouse and open field trials to select those potentially adoptable in sustainable agriculture.

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Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput

Cited by 13 publications

References 51 publications

Tracking and plant growth‐promoting effect of Paraburkholderia tropica MTo‐293 applied to Solanum lycopersicum

Tracking and plant growth‐promoting effect of Paraburkholderia tropica MTo‐293 applied to Solanum lycopersicum

Exploiting synergistic interactions ofMedicago sativaL. andParaburkholderia tropicafor enhanced biodegradation of diesel fuel hydrocarbons

Identification of Beneficial Microbial Consortia and Bioactive Compounds with Potential as Plant Biostimulants for a Sustainable Agriculture

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