Parabiosis in Immunobiology

Nisbet, N. W.

doi:10.1111/j.1600-065x.1973.tb00113.x

Cited by 9 publications

(11 citation statements)

References 118 publications

(107 reference statements)

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“…Development of vascular anastomoses at the capillary or small vessel level has often been demonstrated by injecting one member of a parabiotic pair with a dye or an easily recognizable cell and observing the appearance of the material in the other member. Exact measurements of blood exchange have been made by several investigators [9], The experiments of F leming et al [3] are especially pertinent to the present study. After 3-4 months of parabiosis in littermate SpragueDawley rats, the authors estimated the blood exchange between para bionts using 32P-labelled erythrocytes.…”

Section: Discussionmentioning

confidence: 98%

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Venous Changes in Renal Hypertensive Rats: The Role of Humoral Factors

Simon¹

1978

J Vasc Res

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In order to investigate the role of circulating humoral substances in the pathogenesis of venous changes in experimental hypertension, an attempt was made to induce venous changes in rats in parabiosis with two-kidney Goldblatt hypertensive rats. Sham-operated normotensive rats in parabiosis were used as controls. Venous pressure-volume curves were obtained in the pump perfused vascular beds of the lower body of rats by recording the femoral vein pressure rise during rapid infusion of fluid into the inferior vena cava. The water, sodium and potassium content of veins also was measured. Compared to values in normotensive control rats (n = 11), the venous pressure-volume curves of two-kidney Goldblatt hypertensive rats in parabiosis with unoperated rats (n = 13) were shifted in the direction of the pressure axis (p < 0.01), indicating decreased venous capacity. Venous pressure-volume curves in rats in parabiosis with two-kidney Goldblatt hypertensive rats (n = 13) and in rats in parabiosis with sham-operated rats (n = 10) were similar. The venous wall water, sodium and potassium content in two-kidney Goldblatt hypertensive rats (n = 8) and in their unoperated parabiotic mates (n = 8) was increased (p < 0.05) in comparison to that of sham-operated rats in parabiosis (n = 10). These experiments provide evidence for the role of circulating humoral substances in the pathogenesis of abnormal venous wall composition but not of decreased venous capacity in renal hypertensive rats.

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Section: Discussionmentioning

confidence: 98%

“…Parabiosis is an established technique in experimental physiology [2,9]. The success of parabiosis depends on the histocompatibility of the parabionts.…”

Section: Discussionmentioning

confidence: 99%

Venous Changes in Renal Hypertensive Rats: The Role of Humoral Factors

Simon¹

1978

J Vasc Res

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“…1.2% of the average hematocrit of the respective pairs. This observation ruled out parabiotic intoxication manifested by polycythemia-anemia syndrome (Nisbet, 1973).…”

Section: Efficiency Of Vascular Union and Cellular Trafficmentioning

confidence: 93%

The role of hematogenous and intrinsic precursor cells in lymphocyte production in murine bone marrow and thymus

et al. 1991

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It is well recognized that the bone marrow contains cells that can repopulate a depleted thymus as well as cells that can be induced to express phenotypic markers characteristic of T cells. It is not known, however, to what extent thymocytopoiesis in the normal thymus relies on immigrant, bone marrow-derived cells, nor whether some T cell precursors have entered the bone marrow from the circulation. We used the parabiotic system to test whether thymocytopoiesis relies on progenitors intrinsic to the thymus or on cells that enter the organ from the circulation. In the same system, we have also investigated whether Thy-1- bone marrow lymphocytes that respond to phytohemagglutinin (PHA) by proliferation and Thy-1 expression are produced by myelogenous or hematogenous progenitors. Syngeneic CBA/HT6 and CBA/CaJ mice were joined in parabiotic union at 4-6 weeks of age. Cross circulation between the two partners was verified by the equilibration of Evans' blue dye injected into one partner and by the equilibration of PHA-responsive T cells in the spleen of the parabionts. Chromosome spreads were prepared from the PHA-stimulated T cell-depleted bone marrow and from spontaneously proliferating thymocytes as well as from thymocytes stimulated by PHA or Concanavalin A (Con A). The exchange of spleen colony-forming units (CFU-S) in the femoral marrow was assessed by karyotyping individual spleen colonies. Regardless of the length of parabiotic union, ranging from 4 to 20 weeks, Thy-1-, PHA-responsive bond marrow lymphocytes remained predominantly of the host type with only 3% being derived from the opposite partner.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…In fact, interest in studies of the effects of parabiosis on the processes of normal aging and longevity may have emerged as much from the observation that parabiosis to healthy animals could extend the lifespan of animals that would otherwise succumb earlier due to disease or lethal treatment of some sort. For example, the lethality of irradiation was shown to be rescued by parabiotic pairings, by allowing survival during recovery of the intestinal epithelium and the hematopoietic systems (Finerty et al, 1952; Binhammer et al , 1953; Warren et al , 1960; Carroll & Kimeldorf, 1967, 1969), the latter occurring by the seeding of the hematopoietic system with cells from the healthy parabiont and thus providing evidence in support of the basic notion of stem cells being responsible for maintenance of hematopoiesis (Nisbet, 1973). Parabiosis to a healthy partner was shown to extend lifespan of mice with a form of muscular dystrophy (Hall et al , 1959).…”

Section: Heterochronic Parabiosismentioning

confidence: 99%

Heterochronic parabiosis: historical perspective and methodological considerations for studies of aging and longevity

2013

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Summary Pairing two animals in parabiosis to test for systemic or circulatory factors from one animal affecting the other animal has been used in scientific studies for at least 150 years. These studies have led to advances in fields as diverse as endocrinology, immunology, and oncology. A variation on the technique, heterochronic parabiosis, whereby two animals of different ages are joined to test for systemic regulators of aspects of aging or age-related diseases also has almost a century-long scientific history. In this review, we focus on the history of heterochronic parabiosis, methodological considerations and caveats, and the major advances that have emerged from those studies, including recent advances in our understanding of stem cell aging.

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Parabiosis in Immunobiology

Cited by 9 publications

References 118 publications

Venous Changes in Renal Hypertensive Rats: The Role of Humoral Factors

Venous Changes in Renal Hypertensive Rats: The Role of Humoral Factors

The role of hematogenous and intrinsic precursor cells in lymphocyte production in murine bone marrow and thymus

Heterochronic parabiosis: historical perspective and methodological considerations for studies of aging and longevity

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