Parabens disrupt non-canonical inflammasome activation

Ahn, Huijeong; Lee, Hansae; Lee, Gilyoung; Park, Jeong-Ho; Sung, Hsing‐Wen; Lee, Eunsong; Lee, Geun‐Shik

doi:10.1016/j.intimp.2021.108196

Cited by 5 publications

(7 citation statements)

References 21 publications

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“…The cells were incubated with DMEM media containing 10% fetal bovine serum (FBS, S 001-01, Welgene Inc., Gyeongsan-si, Korea), 50% of L929 cell-conditioned medium as a source of macrophage colony-stimulating factor, and antibiotics at 37 °C in a 5% CO 2 atmosphere for seven days. THP-1 cells (40202, Seoul, Korea, Korean Cell Line Bank), human monocyte-like cells, were maintained in RPMI 1640 media containing 10% FBS and antibiotics [ 5 ]. These cells were differentiated into macrophage-like cells by a treatment of 200 nM phorbol 12-myristate 13-acetate (PMA, InvivoGen, San Diego, CA, USA) for 24 h.…”

Section: Methodsmentioning

confidence: 99%

“…As shown in Figure 1 B, macrophages (i.e., BMDMs and PMA-treated THP-1 cells) were seeded on a 12-well plate (1.0 × 10 6 cells per well) in RPMI 1640 containing 10% FBS and antibiotics, and treated with LPS (1 μg/mL; L4130, Sigma-Aldrich Co., Burlington, MA, USA) for 3 h [ 5 , 9 , 10 ]. The LPS-primed cells were replaced with RPMI 1640 media (350 μL per well) containing an inflammasome trigger with maltol (ESFOOD, #186785643, Gunpo-si, Gyeonggi-do, Korea).…”

Section: Methodsmentioning

confidence: 99%

“…The Casp1 or caspase-4 (Casp4) activities were assayed using recombinant human Casp1 or Casp4 (one unit per reaction, Biovision, Milpitas, CA, USA) and a Casp1 fluorometric assay kit (Biovision, Zurich, Switzerland) or Ac-LEVD-AMD, a substrate of Casp4, (Enzo Life Sciences, Inc., Farmingdale, NY, USA) in the presence of maltol or the pan-caspase inhibitor (Z-VAD-FMK, 10 μg/mL; R&D System, Minneapolis, MN, USA) [ 5 ]. The plates were measured at excitation and emission wavelengths of 400 and 505 nm for Casp1, or 360 and 450 nm for Casp4, using the multi-microplate spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

“…Otherwise, NLRC4 and AIM2 inflammasomes are assembled when the sensor protein is in contact with specific triggers in the cytoplasm, such as the flagellin and dsDNA [ 4 ]. In general, the lipopolysaccharide (LPS) interacts with toll-like receptor (TLR) 4 on the plasma membrane, leading to an inflammatory response through nuclear factor (NF)-κB signaling, but cytosolic LPS is sensed directly by caspase-11 (caspase-4/5 in humans), triggering inflammasome signaling, which is called the non-canonical (NC) inflammasome [ 4 , 5 ]. The activated Casp1 resulting from inflammasome activation proteolytically cleaves the pro-form of cytokines (e.g., interleukin [IL]-1β, and IL-18) and gasdermin D (GSDMD), and induces inflammatory cell death (i.e., pyroptosis) [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

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Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome Activation

et al. 2022

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Maltol (3-hydroxy-2-methyl-4-pyrone) is used widely as a food and cosmetic supplement, and it has antioxidant and anti-inflammatory activities. Inflammasome causes the maturation and secretion of interleukin (IL)-1β and -18 through the activation of caspase-1 (Casp1), which contributes to various inflammatory diseases. This study examined the effects of maltol on the inflammasome activation in macrophages and mice. Lipopolysaccharide (LPS)-primed macrophages were treated with a trigger of NLRP3, NLRC4, AIM2, or non-canonical (NC) inflammasomes in the presence of maltol. The secretion of IL-1β and IL-18 and the cleavage of Casp1 were analyzed as indices of inflammasome activation. Mice were injected with LPS and an NLRP3 trigger with or without maltol, and the peritoneal IL-1β secretions were observed. The effects of maltol on reactive oxygen species (ROS) production and Casp1 activity were analyzed to determine the mechanism. Maltol inhibited the activation of NLRP3 and NC inflammasomes, but it did not alter the other inflammasomes. Maltol also attenuated IL-1β secretion resulting from the inflammasome activation in mice. The anti-inflammatory mechanism of maltol was revealed by the inhibition of ROS production and Casp1 activity. Maltol is suggested to be promising as a anti-inflammasome molecule.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome Activation

et al. 2022

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“…This pyroptosis interrupts the infection of the host cells by microbes 3 , 4 . Unlike the above canonical inflammasomes, murine caspase-11 (caspase-4/5 for humans) interacts with cytosolic lipopolysaccharide (LPS) and then induces pyroptosis by GSDMD cleavage and activates NLRP3 inflammasome indirectly, making it a non-canonical inflammasome 5 – 7 . For successful inflammasome activation, the inflammasome components and substrates need to be upregulated 1 , 8 .…”

Section: Introductionmentioning

confidence: 99%

JC2-11, a benzylideneacetophenone derivative, attenuates inflammasome activation

Lee

Ahn

Yun

et al. 2022

Sci Rep

Self Cite

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Dysregulation of inflammasome activation induces chronic and excess inflammation resulting in several disorders, such as metabolic disorders and cancers. Thus, screening for its regulator derived from natural materials has been conducted progressively. JC2-11 (JC) was designed to enhance the antioxidant activity based on a chalcone, which is abundant in edible plants and a precursor of flavonoids. This study examined the effects of JC on inflammasome activation in human and murine macrophages. JC inhibited the secretion of interleukin (IL)-1β and lactate dehydrogenases, and the cleavage of caspase-1 and gasdermin D in response to the tested activators (i.e., NLRP3, NLRC4, AIM2, and non-canonical inflammasome triggers). In addition, JC attenuated IL-1β secretion from lipopolysaccharide (LPS)-injected mice, an inflammasome-mediating disease model. Mechanistically, JC blocked the expression of the inflammasome components during the priming step of the inflammasome, and interrupted the production of mitochondrial reactive oxygen species. In addition, JC inhibited the activity of caspase-1. In conclusion, JC may be a candidate pan-inflammasome inhibitor.

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Development of PE‐based antibacterial and antioxidant films with L‐ascorbic acid‐p‐hydroxybenzoate addition

Tang,

Zhu,

et al. 2023

Packag Technol Sci

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In this work, a PE‐based antibacterial and antioxidant material was successfully developed. L‐Ascorbic acid and p‐hydroxybenzoic acid were used as raw materials to synthesize L‐ascorbic acid‐p‐hydroxybenzoate (LPHB) by direct esterification method. LPHB was added to polyvinyl alcohol (PVA) solution and coated on corona‐treated low‐density polyethylene (LDPE) film coated with primer layer to prepare a composite film. The effects of different concentrations of LPHB on the performance of composite films were investigated to determine the optimal amount of addition, and then to prepare both antibacterial and antioxidant films. The antimicrobial properties of LPHB were analysed. The antibacterial, antioxidant, mechanical, barrier properties and morphology of the films were characterized and analysed. The results showed that the addition of LPHB significantly improved the antibacterial and anti‐mould properties, oxidation resistance, and barrier properties of the composite film compared to the LDPE film. The best overall performance of the composite film was achieved when the content of LPHB was 1.5%, the antibacterial rate against Escherichia coli and Staphylococcus aureus reached 99%, and the free radical scavenging rate reached 67.22%. In addition, the cling film added with LPHB has better antioxidant and antibacterial properties, which has obvious effect on bread preservation and has potential for application in food packaging.

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Parabens disrupt non-canonical inflammasome activation

Cited by 5 publications

References 21 publications

Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome Activation

Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome Activation

JC2-11, a benzylideneacetophenone derivative, attenuates inflammasome activation

Development of PE‐based antibacterial and antioxidant films with L‐ascorbic acid‐p‐hydroxybenzoate addition

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