Para-Aminobenzoic Acid, Calcium, and c-di-GMP Induce Formation of Cohesive, Syp-Polysaccharide-Dependent Biofilms in Vibrio fischeri

Dial, Courtney N.; Speare, Lauren; Sharpe, Garrett C; Gifford, Scott M.; Septer, Alecia N.; Visick, Karen L.

doi:10.1128/mbio.02034-21

Cited by 13 publications

(18 citation statements)

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“…S5B ). However, the high c-di-GMP condition revealed a role for the bcs (cellulose synthase) locus in regulating syp (mirroring results obtained in specific agar conditions (see reference 52 ) ( Fig. 6A ; see also Fig.…”

Section: Discussionsupporting

confidence: 76%

High Levels of Cyclic Diguanylate Interfere with Beneficial Bacterial Colonization

Isenberg

Christensen

Visick

et al. 2022

mBio

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Section: Discussionsupporting

confidence: 76%

High Levels of Cyclic Diguanylate Interfere with Beneficial Bacterial Colonization

Isenberg

Christensen

Visick

et al. 2022

mBio

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“…S5B ). However, the high c-di-GMP condition revealed a role for the bcs (cellulose synthase) locus in regulating syp (mirroring results obtained in specific agar conditions in (52)) ( Fig. 6A, Fig.…”

Section: Discussionsupporting

confidence: 74%

“…The multiple DGC and PDE mutants were constructed as follows. First, single gene deletions were generated using PCR-SOE and tfoX -induced transformation methods previously described (Pollack-Berti et al, 2010; Visick et al, 2018); some of these mutants have been previously described (Christensen et al, 2020; Dial et al, 2021b). Briefly, regions flanking a gene of interest were amplified using primers listed in Table 3 and joined to an antibiotic-resistance cassette (e.g., Trim R /Erm R /Spec R ) flanked by Flp-recombinase target (FRT) sites that was amplified with primers 2089 and 2090.…”

Section: Methodsmentioning

confidence: 99%

“…described Dial et al, 2021b). Briefly, regions flanking a gene of interest were amplified using primers listed in Table 3 and joined to an antibiotic-resistance cassette (e.g., Trim R /Erm R /Spec R ) flanked by Flp-recombinase target (FRT) sites that was amplified with primers 2089 and 2090.…”

Section: Construction Of Multiple Gene Deletion Mutantsmentioning

confidence: 99%

“…Liquid media was inoculated with single colonies and grown at 25°C for 24 hr on a shaker. 4 μL of overnight cultures were spotted onto TBS or TBS-Ca 2+ agar plates and grown at 25°C for 24 hr (Dial et al, 2021b). Spots were imaged using the Zeiss Axio Zoom.v16 large-field 561 fluorescent stereo microscope and fluorescence levels were measured using the Zen Blue Software polygon tool.…”

Section: Syp and Bcs Expression Analysis In Vitromentioning

confidence: 99%

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High levels of cyclic diguanylate interfere with beneficial bacterial colonization

Isenberg

Christensen

Visick

et al. 2022

Preprint

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During colonization of the Hawaiian bobtail squid (Euprymna scolopes), Vibrio fischeri bacteria undergo a lifestyle transition from a planktonic motile state in the environment to a biofilm state in host mucus. Cyclic diguanylate (c-di-GMP) is a cytoplasmic signaling molecule that is important for regulating motility-biofilm transitions in many bacterial species. V. fischeri encodes 50 proteins predicted to synthesize and/or degrade c-di-GMP, but a role for c-di-GMP regulation during host colonization has not been investigated. We examined strains exhibiting either low or high levels of c-di-GMP during squid colonization and found that while a low c-di-GMP strain had no colonization defect, a high c-di-GMP strain was severely impaired. Expression of a heterologous c-di-GMP phosphodiesterase restored colonization, demonstrating that the effect is due to high c-di-GMP levels. In the constitutive high c-di-GMP state, colonizing V. fischeri exhibited reduced motility, altered biofilm aggregate morphology, and a transcriptional regulatory interaction between two key polysaccharides, cellulose and Syp. Our results highlight the importance of proper c-di-GMP regulation during beneficial animal colonization, illustrate multiple pathways regulated by c-di-GMP in the host, and uncover an interplay of multiple exopolysaccharide systems in host-associated aggregates.

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The type-VI secretion system of the beneficial symbiont Vibrio fischeri

Guckes

Miyashiro

2023

Microbiology

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The mutualistic symbiosis between the Hawaiian bobtail squid Euprymna scolopes and the marine bacterium Vibrio fischeri is a powerful experimental system for determining how intercellular interactions impact animal–bacterial associations. In nature, this symbiosis features multiple strains of V. fischeri within each adult animal, which indicates that different strains initially colonize each squid. Various studies have demonstrated that certain strains of V. fischeri possess a type-VI secretion system (T6SS), which can inhibit other strains from establishing symbiosis within the same host habitat. The T6SS is a bacterial melee weapon that enables a cell to kill adjacent cells by translocating toxic effectors via a lancet-like apparatus. This review describes the progress that has been made in understanding the factors that govern the structure and expression of the T6SS in V. fischeri and its effect on the symbiosis.

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Para-Aminobenzoic Acid, Calcium, and c-di-GMP Induce Formation of Cohesive, Syp-Polysaccharide-Dependent Biofilms in Vibrio fischeri

Abstract: Bacteria integrate environmental signals to regulate gene expression and protein production to adapt to their surroundings. One such behavioral adaptation is the formation of a biofilm, which can promote adherence and colonization and provide protection against antimicrobials.

Cited by 13 publications

References 58 publications

High Levels of Cyclic Diguanylate Interfere with Beneficial Bacterial Colonization

High Levels of Cyclic Diguanylate Interfere with Beneficial Bacterial Colonization

High levels of cyclic diguanylate interfere with beneficial bacterial colonization

The type-VI secretion system of the beneficial symbiont Vibrio fischeri

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