Papillomavirus Replication

Culleton, Sara P.; Androphy, Elliot J.; Kanginakudru, Sriramana

doi:10.1007/978-3-319-21100-8_5

Cited by 1 publication

(2 citation statements)

References 177 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The genome contains six early genes ( E1 – E7 ) that support viral DNA replication and two late genes ( L1 and L2 ) that encode capsid proteins necessary for viral assembly and release. The compact viral genome necessitates viral dependence on host factors to carry out the life cycle, thus viral replication is linked to that of host cells [ 27 , 28 ]. Virally encoded oncogenes do not cause direct transformation of host cells, but induce immune evasion and aberrant cellular proliferation scenarios that support genomic structural aberrations and mutations [ 29 ].…”

Section: Human Papillomavirusesmentioning

confidence: 99%

“…In particular, the viral oncogenes E6 and E7 cooperate to induce cellular immortalization and genomic instability, affecting multiple factors involved in cell cycle progression, cell survival, differentiation, and inhibition of apoptosis [ 30 ]. Corrupted DNA damage response and repair pathways also cooperate to promote a cellular environment tolerant to mutagenesis and ultimately tumorigenesis [ 28 , 29 ].…”

Section: Human Papillomavirusesmentioning

confidence: 99%

See 1 more Smart Citation

Current Technologies and Recent Developments for Screening of HPV-Associated Cervical and Oropharyngeal Cancers

Shah

Senapati

Klacsmann³

et al. 2016

Cancers

View full text Add to dashboard Cite

Mucosal infection by the human papillomavirus (HPV) is responsible for a growing number of malignancies, predominantly represented by cervical cancer and oropharyngeal squamous cell carcinoma. Because of the prevalence of the virus, persistence of infection, and long latency period, novel and low-cost methods are needed for effective population level screening and monitoring. We review established methods for screening of cervical and oral cancer as well as commercially-available techniques for detection of HPV DNA. We then describe the ongoing development of microfluidic nucleic acid-based biosensors to evaluate circulating host microRNAs that are produced in response to an oncogenic HPV infection. The goal is to develop an ideal screening platform that is low-cost, portable, and easy to use, with appropriate signal stability, sensitivity and specificity. Advances in technologies for sample lysis, pre-treatment and concentration, and multiplexed nucleic acid detection are provided. Continued development of these devices provides opportunities for cancer screening in low resource settings, for point-of-care diagnostics and self-screening, and for monitoring response to vaccination or surgical treatment.

show abstract

Section: Human Papillomavirusesmentioning

confidence: 99%