Papillomavirus infections in the oral and genital mucosa of asymptomatic women

Abstract

Human papillomavirus (HPV) has been found in several regions of the body, including the oral cavity. Recently, this virus has been associated with oropharyngeal cancer, but little is known about HPV transmission to the oral cavity. We carried out a study to investigate concurrent oral and cervical infections in 76 asymptomatic women attending a healthcare program. Demographic and behavior data were obtained through a structured questionnaire. Oral and cervical mucosa scrapings were collected and stored for DNA extraction. HPV DNA amplification was performed by polymerase chain reaction assay (PCR) using both primers My09/My11 and FAP59/64, followed by HPV typing with restriction fragment length polymorphism analysis (RFLP) and sequencing. The data collected revealed no risk factors for HPV infection in these 76 women. HPV prevalence of 9.2 and 5.3% was found in cervical and oral mucosa, respectively. Concurrent infections by discordant types were detected in one case only. Sequencing procedures allowed us to detect a new putative HPV 17 subtype from the Betapapillomavirus genus. Our results support the view that cervical and oral HPV infections are independent events. The observed low prevalence of both oral and cervical HPV infections could be associated with attendance in a healthcare program.

Identification of human papillomavirus in oral rinse specimens from women with and without cervical intraepithelial lesions

“…Our study showed a 3.9% frequency for HPV in oral rinse. This finding is similar to previous studies that showed 3.2%–5.3% frequency of oral HPV infection in women without oral lesions 8 9. An Italian study that included 98 women with cervical HPV infection had a 14.3% frequency for oral HPV, while a metanalysis done by the group obtained a 18.1% frequency 10.…”

Low Rates of Dual-Site and Concordant Oral-Cervical Human Papillomavirus Infections and Cancers: A Systematic Review

“…Eighty-three percent of all synchronous testing studies (n=67/80, Table 3A ) ( 4 , 15 – 21 , 23 , 25 – 27 , 29 – 44 , 47 , 49 – 52 , 54 – 65 , 67 – 78 , 84 – 93 ) provided some form of data on concurrent oral-cervical HPV+ cases. Concurrent oral and cervical HPV infection rates could be calculated for most, but not all, of these studies (n=59/67, 88%) ( 4 , 15 – 17 , 20 , 21 , 23 , 25 – 27 , 29 – 39 , 41 – 44 , 47 , 49 – 52 , 54 – 65 , 67 – 72 , 76 – 78 , 84 – 89 , 91 – 93 ). The calculated concurrent rates ranged from 0% to 95%, depending on the study.…”

“…On average, 15% of women had HPV infections occurring concurrently in both sites. Most rates of concurrent oral and cervical HPV infections were ≤10% (n=39/59, 66%) ( 15 , 17 , 20 , 21 , 23 , 26 , 27 , 29 – 32 , 34 – 37 , 39 , 41 , 43 , 44 , 49 , 51 , 52 , 55 , 56 , 59 – 65 , 67 , 69 , 77 , 78 , 84 – 86 , 92 ). Only four studies (7%) ( 54 , 72 , 89 , 93 ) had concurrent oral and cervical HPV infection rates over 65%.…”

Detección del virus del papiloma humano en mucosa oral de mujeres de Cali, Colombia

“…Un estudio previo en Colombia, se reportó para la ciudad de Cali la presencia del VPH-16 en un 43.8% en mucosa oral de 43 mujeres sanas mayores de 18 15 , lo anterior no concuerda con nuestros resultados de 6.6%, ni con lo reportado para otros países de Suramérica como Perú y Brasil, los cuales han detectado el VPH en mucosa oral de mujeres sanas en porcentajes entre un 5 al 6% 16,17 . Además, no se identificó genotipos de alto riesgo oncogénico como el VPH-16.…”