Paper platform for determination of bumetanide in human urine samples to detect doping in sports using digital image analysis

Liquid chromatography–tandem mass spectrometry determination of bumetanide in human plasma and application to a clinical pharmacokinetic study

“…Additionally, BUM inhibits the chloride cotransporter NKCCl (cation‐chloride cotransporter) to maintain steady intracellular (Cl − ) levels in neurons (Greger & Wangemann, 1987; Löscher & Kaila, 2022; Zhao et al, 2022). There are many ways to analyze BUM in various biological matrices, either on their own (Bökens et al, 1988; Legorburu et al, 2001; Walmsley et al, 1981; Wells et al, 1991) or in conjunction with other diuretics for anti‐doping studies (dos Santos Ruy et al, 2014; Li et al, 2011; Luiz et al, 2019), employing a variety of techniques such as the fluorimetric and high‐performance liquid chromatography (HPLC) methods (Howlett et al, 1984). However, these methods have some inherent disadvantages, including the need for many solvents; the difficulty in validating them on a laboratory scale; laborious separation, derivatization, or incubation procedures; the necessity for large samples; and their lack of sensitivity or specificity.…”

Using a biphasic system and digital imaging analysis with chemometric tools for simultaneous determination of Cu2+ and furfural in cachaça

“…It is worth noting that the analytical curves with R, G or B data vs. concentration are sometimes reported in the literature. 50 The concept of complementary color has been widely used for colorimetry and/or spectrophotometry analyses. 1,51,52 However, it should be noted that the captured signal from a digital camera stems from reection; thus, the reected signal based on R, G or B value is correlated with the absorbance.…”

Research on Detection of Sterol Doping in Sports by Electrochemical Sensors: A Review

“…Diuretics rapidly reduce the concentration of other stimulants and their metabolites in body uids and excreta in a short time, a ecting the accuracy of drug test results. Diuretics can interfere with drug testing, resulting in false negative omissions [10][11][12][13]. Diuretics and masking agents are high-incidence stimulants.…”