Paper microfluidic extraction and direct smartphone-based identification of pathogenic nucleic acids from field and clinical samples

Fronczek, Christopher F.; Park, Tu San; Harshman, Dustin K.; Nicolini, Ariana M.; Yoon, Jeong Yeol

doi:10.1039/c3ra47688j

Cited by 101 publications

(60 citation statements)

References 21 publications

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“…However, while these systems are portable and easy to use, they require microfabrication and are not optimized for multiplexing or multiple target detection. More recently, paper microfluidic devices have been described for a multitude of assays (Cahrillo et al, 2009;Martinez et al, 2008;Nie et al, 2010), including a highly sensitive and rapid multiplexed platform detecting Salmonella in poultry packaging, whole blood and feces utilizing a smartphone (Fronczek et al, 2014). While these systems have greatly improved on previous soft lithography-based microfluidics technology in terms of fabrication, ease-of-use, and portability, they still require expensive reagents and show some experimental variation due to inconsistencies in the backscattering of the paper fibers.…”

Section: Introductionmentioning

confidence: 98%

“…Good progress has been made in these efforts, specifically in bacterial assays. Direct bacterial nucleic acid detection has been attempted in lateral flow format (Mao et al, 2009;Lo et al, 2013;Baeumner, 2004) and paper microfluidic format (Fronczek et al, 2014;Costa et al, 2014), and these assays are fairly rapid and show good sensitivity, with the limit of detection being typicallyo10 3 colony forming units (CFU) per mL. However, these formats often require sample pre-treatment, separate nucleic acid extraction, or separate thermocycling, and sometimes lack specificity.…”

Section: Introductionmentioning

confidence: 98%

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Droplet-based immunoassay on a ‘sticky’ nanofibrous surface for multiplexed and dual detection of bacteria using smartphones

Nicolini

Fronczek

Yoon

2015

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Droplet-based immunoassay on a ‘sticky’ nanofibrous surface for multiplexed and dual detection of bacteria using smartphones

Nicolini

Fronczek

Yoon

2015

Biosensors and Bioelectronics

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“…At the entrance of the sample preparation stage, the channel will split in two: one channel for nucleic acid extraction and the other for particle-conjugated antibody capture. Nucleic acid extraction will be accomplished through paper microfluidics, 184 during which the nucleic acid will be tagged with preloaded fluorophores. The channel will split into several subchannels of preloaded nucleotides specific for a range of targets of interest (different targets in different channels), and a process of positive selection will be used to sort specific pathogens.…”

Section: A Portable Rapid and Sensitive Multiplexed Sensor For Bioamentioning

confidence: 99%

Biosensors for Monitoring Airborne Pathogens

Fronczek

Yoon

2015

SLAS Technology

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Airborne pathogens affect both humans and animals and are often highly and rapidly transmittable. Many problematic airborne pathogens, both viral (influenza A/H1N1, Rubella, and avian influenza/H5N1) and bacterial (Mycobacterium tuberculosis, Streptococcus pneumoniae, and Bacillus anthracis), have huge impacts on health care and agricultural applications, and can potentially be used as bioterrorism agents. Many different laboratory-based methods have been introduced and are currently being used. However, such detection is generally limited by sample collection, including nasal swabs and blood analysis. Direct identification from air (specifically, aerosol samples) would be ideal, but such detection has not been very successful due to the difficulty in sample collection and the extremely low pathogen concentration found in aerosol samples. In this review, we will discuss the portable biosensors and/or micro total analysis systems (µTAS) that can be used for monitoring such airborne pathogens, similar to smoke detectors. Current laboratory-based methods will be reviewed, and possible solutions to convert these lab-based methods into µTAS biosensors will be discussed.

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“…Effective integration of the two key technologies critically empowers MS 2 for many mobile sensing applications. Current applications of MS 2 cover detection of various environmental and health indicators such as pH (Lopez-Ruiz et al 2014), nitrite , heavy metal (Chen et al 2014b;Wang et al 2014), bacterial contamination (Hutchison et al 2015;San Park et al 2013;Zhu et al 2012), blood glucose (Chun et al 2014), proteins (Chan et al 2015;Lillehoj et al 2013;Preechaburana et al 2012;You et al 2013) and other pathogen-associated biomarkers (Fronczek et al 2014;Stemple et al 2014;Yeo et al 2016). Some complicated assays such as enzyme-linked immunosorbent assay (ELISA) (Chen et al 2014a;Wang et al 2011) and polymerase chain reaction (PCR) Liao et al 2016;Stedtfeld et al 2012) were successfully implemented with the MS 2 systems.…”

Section: Introductionmentioning

confidence: 99%

Mkit: A cell migration assay based on microfluidic device and smartphone

Yang

Peretz‐Soroka

et al. 2018

Biosensors and Bioelectronics

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Mobile sensing based on the integration of microfluidic device and smartphone, so-called MS 2 technology, has enabled many applications over recent years, and continues to stimulate growing interest in both research communities and industries. In particular, it has been envisioned that MS 2 technology can be developed for various cell functional assays to enable basic research and clinical applications. Toward this direction, in this paper, we describe the development of a MS 2 -based cell functional assay for testing cell migration (the M kit ). The system is constructed as an integrated test kit, which includes microfluidic chips, a smartphone-based imaging platform, the phone apps for image capturing and data analysis, and a set of reagent and accessories for performing the cell migration assay. We demonstrated that the M kit can effectively measure purified neutrophil and cancer cell chemotaxis. Furthermore, neutrophil chemotaxis can be tested from a drop of whole blood using the M kit with red blood cell (RBC) lysis. The effects of chemoattractant dose and gradient profile on neutrophil chemotaxis were also tested using the M kit . In addition to research applications, we demonstrated the effective use of the M kit for on-site test at the hospital and for testing clinical samples from chronic obstructive pulmonary disease patient. Thus, this developed M kit provides an easy and integrated experimental platform for cell migration related research and potential medical diagnostic applications.

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Paper microfluidic extraction and direct smartphone-based identification of pathogenic nucleic acids from field and clinical samples

Cited by 101 publications

References 21 publications

Droplet-based immunoassay on a ‘sticky’ nanofibrous surface for multiplexed and dual detection of bacteria using smartphones

Droplet-based immunoassay on a ‘sticky’ nanofibrous surface for multiplexed and dual detection of bacteria using smartphones

Biosensors for Monitoring Airborne Pathogens

Mkit: A cell migration assay based on microfluidic device and smartphone

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