Paper Chromatographic Determination of Tetracycline

Youssef, Muhammad; Ibrahim, El.A.; Attia, I.A.

doi:10.1002/jps.2600621222

Cited by 11 publications

(5 citation statements)

References 11 publications

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“…Low Rl values or diffuse spots were observed by separate use of either urea or edetate disodium solutions, respectively. These results were consistent with those previously observed for tetracycline (2). Table I presents This procedure was successfully applied to chlortetracycline separation from either tetracycline or oxytetracycline (Table I).…”

Section: Resultssupporting

confidence: 91%

“…Chlortetracycline was characterized by the highest Rf value, followed in decreasing order by tetracycline and oxytetracycline. Complex formation between tetracycline or oxytetracycline and the components of the impregnating solution was previously proved (1,2). When chlortetracycline was eluted from the chromatogram using pH 5.0 McIlvaine buffer, it showed a shift of toward the shorter wavelength, thus confirming antibiotic complexation on the chromatogram.…”

Section: Resultsmentioning

confidence: 62%

“…Previously (1,2), paper chromatographic methods were developed for the quantitative determination of oxytetracycline and tetracycline.…”

mentioning

confidence: 99%

“…Urea was reported to increase the water solubility of chlortetracycline through complex formation (3,4). The use of a mixture of urea and edetate disodium aqueous solutions as an immobile phase was successfully applied to the paper chromatographic determination of tetracycline in pharmaceutical formulations (2). The objective of the present investigation was to establish a precise quantitative paper chromatographic determination of chlortetracycline in various dosage forms and in the presence of its degradation products.…”

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confidence: 99%

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Paper Chromatographic Determination of Chlortetracycline

Youssef

Attia

Safwat

1978

Journal of Pharmaceutical Sciences

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 62%

“…Previously (1,2), paper chromatographic methods were developed for the quantitative determination of oxytetracycline and tetracycline.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

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Paper Chromatographic Determination of Chlortetracycline

Youssef

Attia

Safwat

1978

Journal of Pharmaceutical Sciences

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“…Chlortetracycline-hydrochloride (chlorotetracycline) was purchased from Calbiochem and determined to Abbreviations: doxyldecane, 2,2-dimethyl-4-butyl-4-pentyl-N-oxyloxazolidine; ESR, electron spin resonance. * be greater than 98% pure as measured by paper chromatography (15). The N-phenyl-1-naphthylamine was purchased from Eastman Organic Chemicals and was twice recrystallized from acetone before use.…”

Section: Methodsmentioning

confidence: 99%

Membrane lateral phase separations and chlortetracycline transport by Bacillus megaterium

Dockter

Trumble

Magnuson

1978

Proc. Natl. Acad. Sci. U.S.A.

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Chlortetracycline, a fluorescent robe of its own active transport, has been used to study lateral phase sep- Tetracyclines are broad-spectrum antibiotics that bind to the bacterial ribosome, where they specifically inhibit protein biosynthesis (1, 2). The antibiotic is most likely accumulated within bacteria by an active transport system, which may account for the specificity of the drug (3). In several recent papers, we have shown that tetracyclines can be used as fluorescent probes of their own transport through bacterial membranes (4-7). Qualities that make them ideal for transport studies are the specificity of binding of the probe to the transport site in the bacterial membrane and the large increase in the quantum yield of fluorescence when tetracyclines bind to these apolar regions of the membrane through a divalent cation complex. The latter property results in great sensitivity for detection of the probe without perturbation of membrane integrity. Monitoring the tetracycline fluorescence allows one to follow antibiotic accumulation continuously. We have previously used the fluorescence to demonstrate saturation of the tetracycline transport system in Staphylococcus aureus and to examine the role of the membrane lipid in this transport system (4, 5). The temperature dependence of the transport rate, as measured by fluorescence enhancement, and tetracycline mobility changes, as measured by fluorescence polarization, were used in these studies (5). (14). Transesterification of the fatty acids with boron trifluoride/methanol followed by gas chromatography on a diethylene glycol succinate column at 1700 showed that the major fatty acids of these cells were the branched-chain fatty acids, anteiso and iso-C13

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