Paper-Based Quantification of Male Fertility Potential

Nosrati, Reza; Gong, Ming; Gabriel, Maria C. San; Pedraza, Claudio E.; Zini, Armand; Sinton, David

doi:10.1373/clinchem.2015.250282

Cited by 60 publications

(54 citation statements)

References 41 publications

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“…Our paper-based MTT assay experimental results suggest that MTT color intensity changes by MTT reduction could be correlated to sperm motility, so this system may be applicable for fertility examinations1819. These results were consistent with other research groups reporting on the use of paper-based MTT assays for human sperm motility20. Physiological functions, especially redox chemistry with exogenous compounds such as MTT, are not well discussed regarding porcine sperm to date and additional investigation of this sperm detection technology is required for implementation in cattle breeding.…”

supporting

confidence: 91%

“…We show that paper-based MTT can evaluate porcine sperm motility in a manner similar to that reported for evaluating human sperm motility181920. Paper-based assay systems have an advantage over iSperm systems because of the lower initial cost; iSperm systems and optical microscopy detection systems require expensive equipment as shown in Table 2.…”

Section: Resultssupporting

confidence: 54%

“…To find the clues to this relationship, we must first touch on mammalian sperm ATP production mechanisms, as they provide energy for sperm motility. Sperm ATP is produced by an electron transfer system in mitochondria and a glycolysis system in flagellum cytosol, and the origin of ATP for mammalian sperm motility is currently under debate202122. Recent studies report that ATP produced by glycolysis played a key role in mouse sperm motility based on knock-out mice or chemical inhibition studies2324.…”

mentioning

confidence: 99%

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Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Matsuura

Huang

Chen

et al. 2017

Sci Rep

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Mammalian sperm motility has traditionally been analyzed to determine fertility using computer-assisted semen analysis (CASA) systems. To develop low-cost and robust male fertility diagnostics, we created a paper-based MTT assay and used it to estimate motile sperm concentration. When porcine sperm motility was inhibited using sperm enzyme inhibitors for sperm enzymes related to mitochondrial activity and glycolysis, we simultaneously recorded sperm motility and enzymatic reactivity using a portable motility analysis system (iSperm) and a paper-based MTT assay, respectively. When using our paper-based MTT-assay, we calculated the area mean value signal intensity (AMV) to evaluate enzymatic reactivity. Both sperm motility and AMV decreased following treatment with iodoacetamide (IODO) and 3-bromopyruvic acid (3BP), both of which are inhibitors of glycolytic enzymes including glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We found a correlation between recorded motility using iSperm and AMV from our paper-based assay (P < 0.05), suggesting that a sperm-related enzymatic reaction is involved in sperm motility. Under this protocol, MTT reduction was coupled with catalysis of GAPDH and was promoted by electron transfer from NADH. Based on this inhibitor study, sperm motility can be estimated using our paper-based MTT-assay.

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supporting

confidence: 91%

Section: Resultssupporting

confidence: 54%

mentioning

confidence: 99%

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Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Matsuura

Huang

Chen

et al. 2017

Sci Rep

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“…However, following storage at room temperature, MOTs of more than 100 million spermatozoa and more than 50 million motile spermatozoa were significantly increased. Similarly, Nosrati et al reported a high correlation between MTT colour intensity changes on their paper-based device and motility of human semen in their clinical study [80]. Based on the results of these clinical studies, this assay can be used to determine numbers of motile sperm, and the results can be presented on a smartphone window in situ.…”

Section: Paper-based Devices For Semen Diagnosismentioning

confidence: 93%

Recent advances in microfluidic diagnostic and treatment systems for assisted reproductive technologies in developing countries

Matsuura¹,

Nishina²,

Asano³

2017

Point-of-Care Diagnostics - New Progresses and Perspectives

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“…One of the most familiar directto-consumer diagnostics tests has been the home pregnancy test. In this issue of Clinical Chemistry, Nosrati et al (1 ) report their clever and practical approach toward developing a new point-of-care diagnostic test for another reproductive health need-the assessment of male fertility.…”

mentioning

confidence: 99%

Toward a Microfluidics-Based Home Male Fertility Test

2016

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Consumers are increasingly interested in knowing more about their own health. One of the most familiar directto-consumer diagnostics tests has been the home pregnancy test. In this issue of Clinical Chemistry, Nosrati et al.(1 ) report their clever and practical approach toward developing a new point-of-care diagnostic test for another reproductive health need-the assessment of male fertility.The authors developed the test using paper, a familiar substrate used in pregnancy tests and the subject of a flurry of recent improvements in microfluidics (2 ). The authors placed at the bottom one laminate layer, and on top, paper layers patterned with wax (3 ). With the paper test they aimed to quantify sperm concentration via a dye that changes color in the presence of an enzyme (diaphorase flavoprotein) that resides within metabolically active human sperm. In one spot on the paper, the sample reacts directly with the dried dye. In a second spot, sperm must swim vertically through a highly viscous buffer and a membrane filter to generate a colorimetric signal. Ten minutes later, a scanner reads the color intensities of the 2 spots, which are used to quantify live and motile sperm concentrations.The paper diagnostic test was assessed on 17 frozen human semen samples obtained from a hospital. Although the performance was promising (for example, it predicted correctly the 1 out of 6 samples having low sperm motility), it is increasingly recognized in the research field of microfluidics that there exists a yawning gap between an academic demonstration and clinical utility. Setting their test apart from many academic demonstrations, these investigators studied the performance of the test over different times of storage and at different humidity levels. Their device, when packaged with desiccant, demonstrated only a small degradation in color change over 16 weeks even in the presence of 22.8 g/m 3 humidity (equivalent to about the maximum humidity at room temperature). For point-of-care use, building a rugged and stable test is critical in translating a promising technology to the clinic.Not all clinical diagnostic tests that can be done in a point-of-care format should be done in a point-of-care format. One driver that justifies a consumer-facing diagnostic test is to provide information that would motivate patients to purchase and use the product. In line with the success of the home pregnancy test, the authors chose an application that would appeal to consumer use. In this case, assessment of male fertility is often initiated by the consumer in cases in which a couple has difficulty conceiving. Moreover, obtaining semen samples would be more comfortably performed at home and in private, which makes this application perfectly suited for home use. Although such considerations may appear more relevant at a later stage of commercialization, understanding them is important at an early research stage to develop a device with proper target specifications (sample type, simplicity of design, resources available) for the intended c...

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Paper-Based Quantification of Male Fertility Potential

Cited by 60 publications

References 41 publications

Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Recent advances in microfluidic diagnostic and treatment systems for assisted reproductive technologies in developing countries

Toward a Microfluidics-Based Home Male Fertility Test

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