Paper-based lateral flow strip assay for the detection of foodborne pathogens: principles, applications, technological challenges and opportunities

Luo, Ke; Kim, Hae‐Yeong; Oh, Mi‐Hwa; Kim, Young-Rok

doi:10.1080/10408398.2018.1516623

Cited by 69 publications

(38 citation statements)

References 93 publications

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“…Primer design and extensive optimization of multiplex nucleic acid amplification incorporated with LFA assays are critical steps to avoid primer dimers and nonspecific results [31]. The coupling of powerful amplification methods with simple detection platforms, especially lateral flow strips, increases the feasibility of their application in point of care or field detection [33]. Of the various LFA formats, the STH-PAS is a lateral flow dipstick format that allows the detection of…”

Section: Plos Onementioning

confidence: 99%

Rapid detection of extended spectrum β-lactamase producing Escherichia coli isolated from fresh pork meat and pig cecum samples using multiplex recombinase polymerase amplification and lateral flow strip analysis

et al. 2021

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The emergence and dissemination of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli is a global health issue. Food-producing animals, including pigs, are significant reservoirs of antimicrobial resistance (AMR), which can be transmitted to humans. Thus, the rapid detection of ESBLs is required for efficient epidemiological control and treatment. In this study, multiplex recombinase polymerase amplification (RPA) combined with a single-stranded tag hybridization chromatographic printed-array strip (STH-PAS), as a lateral flow strip assay (LFA), was established for the rapid and simultaneous detection of multiple bla genes in a single reaction. Visible blue lines, indicating the presence of the blaCTX-M, blaSHV, and blaOXA genes, were observed within 10 min by the naked eye. The limit of detection of all three genes was 2.5 ng/25 μL, and no cross-reactivity with seven commensal aerobic bacteria was observed. A total of 93.9% (92/98) and 96% (48/50) of the E. coli isolates from pork meat and fecal samples, respectively, expressed an ESBL-producing phenotype. Nucleotide sequencing of the PCR amplicons showed that blaCTX-M was the most prevalent type (91.3–95.83%), of which the main form was blaCTX-M-55. The sensitivity and specificity of the RPA-LFA were 99.2% and 100%, respectively, and were in almost perfect agreement (κ = 0.949–1.000) with the results from PCR sequencing. Thus, the RPA-LFA is a promising tool for rapid and equipment-free ESBL detection and may facilitate clinical diagnosis in human and veterinary medicine, as well as AMR monitoring and surveillance.

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Section: Plos Onementioning

confidence: 99%

Rapid detection of extended spectrum β-lactamase producing Escherichia coli isolated from fresh pork meat and pig cecum samples using multiplex recombinase polymerase amplification and lateral flow strip analysis

et al. 2021

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“…After the sample solution is applied, the analytes labeled with the antibody keep moving forward by capillary force toward the pad's detection zone and are identified by the colored or fluorescent signal. The recent trend of incorporating LFSA in foodborne pathogen detection focuses on increasing the device's sensitivity (55,72,90), which may require concentrating the analytes in the sample solution before analysis. One of the typical methods is using antibody-conjugated nanoparticles, which are discussed below.…”

Section: Biosensors Based On Antibody Interaction Optical and Mass-bamentioning

confidence: 99%

Current State of Development of Biosensors and Their Application in Foodborne Pathogen Detection

Bai

Bhunia

2021

Journal of Food Protection

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Foodborne disease outbreaks continue to be a major public health and food safety concern. Ensuring the safety of food prior to retail distribution by testing products promptly can protect consumers from foodborne diseases. F ast, sensitive, and accurate detection tools are in great demand. Therefore, various approaches have been explored in the past few years to find a more effective way to incorporate antibodies, oligonucleotides, phages, and mammalian cells as signal transducers and analyte recognition probes on biosensor platforms. The ultimate goal is to achieve high specificity and low detection limits (1-100 bacterial cells or pico-nanogram levels of toxins). Besides, advancement in mammalian cells and bacteriophage-based sensors led to their ability to detect not only low levels of pathogens but also to differentiate live from dead ones. Combining different biotechnology platforms enabled practical utility and application of biosensors in foodborne pathogen detection. However, further rigorous testing of biosensors from complex food matrices is needed to ensure their utility in point-of-care need and for outbreak investigations.

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“…64,65 When compared to the LFSA approaches, this microfluidic system has a higher sensitivity to achieve a lower LOD within 40 min. 66,67 In contrast, LFSAs have high LODs 66,68 (e.g., 10 4 -10 6 virus particles for influenza A) or additional enrichment steps 67,69 (e.g., 2 CFU with a 48 h incubation for MRSA) 67,69 to overcome this limited sensitivity.…”

Section: The Limit Of Detection Of the Custom-made Portable Systemmentioning

confidence: 99%

A sample-to-answer, portable platform for rapid detection of pathogens with a smartphone interface

Chen

et al. 2019

Lab Chip

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Paper-based lateral flow strip assay for the detection of foodborne pathogens: principles, applications, technological challenges and opportunities

Cited by 69 publications

References 93 publications

Rapid detection of extended spectrum β-lactamase producing Escherichia coli isolated from fresh pork meat and pig cecum samples using multiplex recombinase polymerase amplification and lateral flow strip analysis

Rapid detection of extended spectrum β-lactamase producing Escherichia coli isolated from fresh pork meat and pig cecum samples using multiplex recombinase polymerase amplification and lateral flow strip analysis

Current State of Development of Biosensors and Their Application in Foodborne Pathogen Detection

A sample-to-answer, portable platform for rapid detection of pathogens with a smartphone interface

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