Paper-based chip integrated with vitrified silk fibroin microneedle array for colorimetric detection of histamine in food

Zhu, Lin; Zhang, Jianhong; Zhang, Hao; Zhang, Meng; Wang, Jinyi

doi:10.1016/j.snb.2024.135819

Cited by 2 publications

(1 citation statement)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such SF-based fibers, , films, , sponges, , hydrogels, , and microneedles (MNs) , have demonstrated great potential in regenerative medicine and diagnostics. Used as MNs, SF is a typical biomaterial with high integration properties, presenting a compelling option for constructing an adaptable catalyst for a diagnostic amplification platform, which would not introduce side-influence for following detected matter due to its high biocompatibility . However, the integration of hardness and swelling within one SF microneedle mainly confined its further stability and accuracy for visual detection of glucose, pH, bacteria, ect. − To achieve its stiffness, Wang et al prepared SF MNs by photo-cross-linking, which had a good mechanical strength of 1.59 N/needle, while the swelling ratio was still limited to 8% .…”

Section: Introductionmentioning

confidence: 99%

Surfactant-Boosted Silk Fibroin Microneedle Patches for Visual Glucose Detection

Peng,

Liu,

Zheng

et al. 2024

ACS Appl. Polym. Mater.

View full text Add to dashboard Cite

Silk fibroin (SF) has demonstrated great potential in diagnostics due to its excellent biocompatibility and biodegradability. Used as microneedles (MNs), SF is a compelling option for constructing an adaptable catalyst diagnostic platform due to its high integration. However, the defective hardness and swelling of SF MNs confine further catalyst-based visional detection. Herein, we present a facile approach involving the β-sheet induction by a nonionic surfactant, Tween 20, posing an on-demand regulation of SF MNs for further visual detection. Compared with the usual nonionic surfactants (Tween 80, PEG, and PEGMSE), Tween 20 demonstrated a remarkable ability to induce β-sheet formation within SF, which subsequently could facilitate the distinctive hardness (>1.06 N/needle) and swelling (60−180%) for SF MNs. Then, the agarose-based glucose model was used for visual detection, and consequently, the glucose oxidase (GOx)-HRP-TMB-integrated SF MN patch demonstrated a significant colorimetric quantitation as glucose concentration within 25−600 mg/dL (R 2 = 0.99304). More generally, the visual detection of glucose in types of fruits revealed the high efficacy and accuracy of the SF MN patch (R 2 = 0.94724), further indicating the advantage of Tween 20-tailored SF MNs as a visual platform. These achievements provide a facile tactic to reach an alternative hardness and swelling regulation by one step for catalyst implanted based SF MNs, further revealing the great potential of SF MNs for visual diagnostic.

show abstract