Paper-based cell culture platform and its emerging biomedical applications

Ng, Kelvin; Gao, Bin; Yong, Kar Wey; Li, Yuhui; Shi, Meng; Zhao, Xin; Li, Zedong; Zhang, Xiao Hui; Murphy, Belinda; Yang, Hui; Xu, Feng

doi:10.1016/j.mattod.2016.07.001

Cited by 112 publications

(99 citation statements)

References 83 publications

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“…Flexible sheets may be formed using various techniques including casting elastomeric or intrinsically flexible materials, electrospinning, or by using spin coating or layer‐by‐layer assembly. [2c] Paper has also recently attracted attention due to its porosity and flexibility, while permitting modifications of physical and chemical properties . Electrospinning to form micro‐ and nanofibrous architectures can form sheets (e.g., fiber mats) from a variety of synthetic and natural materials .…”

Section: Introductionmentioning

confidence: 99%

Easy, Scalable, Robust, Micropatterned Silk Fibroin Cell Substrates

Meng

Pradhan

Agostinacchio

et al. 2019

Adv Materials Inter

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Thin polymeric films are being explored for biomedical uses such as drug delivery, biofiltration, biosensors, and tissue regeneration. Of specific interest is the formation of mechanically flexible sheets, which can be formed with controllable thickness for sealing wounds, or as biomimetic cellular constructs. Flexible substrates with precise micro‐ and nanopatterns can function as supports for cell growth with conformal contact at the biointerface. To date, approaches to form free‐standing, thin sheets are limited in the ability to present patterned architectures and micro/nanotextured surfaces. Other materials have a lack of degradability, precluding their application as cellular scaffolds. An approach is suggested using biocompatible and biodegradable films fabricated from silk fibroin. This work presents the fabrication and characterization of flexible, micropatterned, and biodegradable 2D fibroin sheets for cell adhesion and proliferation. A facile and scalable technique using photolithography is shown to fabricate optically transparent, strong, and flexible fibroin substrates with tunable and precise micropatterns over large areas. By controlling the surface architectures, the control of cell adhesion and spreading can be observed. Additionally, the base material is fully degradable via proteolysis. Through mechanical control and directing the adherent cells, it is possible to explore interactions of cells and the microscale geometric topography.

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Section: Introductionmentioning

confidence: 99%

Easy, Scalable, Robust, Micropatterned Silk Fibroin Cell Substrates

Meng

Pradhan

Agostinacchio

et al. 2019

Adv Materials Inter

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“…Paper is composed of cellulose fibers and has porous structures; thus, fluid can be easily transported through the scaffold . Furthermore, the flexible but tough mechanical properties of paper enable multilayer stacking . The first demonstration of paper‐based cell culture platforms was reported by the Whitesides group.…”

Section: Microphysiological Models Of Human Cancermentioning

confidence: 99%

Microphysiological Systems as Enabling Tools for Modeling Complexity in the Tumor Microenvironment and Accelerating Cancer Drug Development

Kim

Habbit

et al. 2019

Adv Funct Materials

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Tumor cell heterogeneity with distinct phenotypes, genotypes, and epigenetic states as well as the complex tumor microenvironment is major challenges for cancer diagnosis and treatment. There have been substantial advances in our knowledge of tumor biology and in the capabilities of available biological analysis tools; however, the absence of physiologically relevant in vitro testing platforms limits our ability to gain an in-depth understanding of the role of the tumor microenvironment in cancer pathology. In this review, recent advances in engineered tumor microenvironments to advance cancer research and drug discovery are presented, including tumor spheroids, microfluidic chips, paper scaffolds, hydrogel-based engineered tissues, 3D bioprinted scaffolds, and multiscale topography. Furthermore, how these technologies address the specific characteristics of the native tumor microenvironment is described. Through the comparison of these biomimetic 3D tumor models to conventional 2D culture models, the validity and physiological relevance of these platforms for fundamental in vitro studies of the tumor biology, as well as their potential use in drug screening applications, is also discussed.

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“…Paper can be stacked into multilayered constructs, which resemble 3D fibrous structure of tissues . Paper is an eco‐friendly and biocompatible material, which is cost efficient to mass produce due to its scalability.…”

Section: Paper‐based Platforms For Bioengineering and Regenerative Mementioning

confidence: 99%

Engineered Paper‐Based Cell Culture Platforms

Lantigua

Kelly

Ünal

et al. 2017

Adv Healthcare Materials

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Paper is used in various applications in biomedical research including diagnostics, separations, and cell cultures. Paper can be conveniently engineered due to its tunable and flexible nature, and is amenable to high-throughput sample preparation and analysis. Paper-based platforms are used to culture primary cells, tumor cells, patient biopsies, stem cells, fibroblasts, osteoblasts, immune cells, bacteria, fungi, and plant cells. These platforms are compatible with standard analytical assays that are typically used to monitor cell behavior. Due to its thickness and porous nature, there are no mass transport limitations to/from the cells in paper scaffolds. It is possible to pattern paper in different scales (micrometer to centimeter), generate modular configurations in 3D, fabricate multicellular and compartmentalized tissue mimetics for clinical applications, and recover cells from the scaffolds for further analysis. 3D paper constructs can provide physiologically relevant tissue models for personalized medicine. Layer-by layer strategies to assemble tissue-like structures from low-cost and biocompatible paper-based materials offer unique opportunities that include understanding fundamental biology, developing disease models, and assembling different tissues for organ-on-paper applications. Paper-based platforms can also be used for origami-inspired tissue engineering. This work provides an overview of recent progress in engineered paper-based biomaterials and platforms to culture and analyze cells.

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Paper-based cell culture platform and its emerging biomedical applications

Cited by 112 publications

References 83 publications

Easy, Scalable, Robust, Micropatterned Silk Fibroin Cell Substrates

Easy, Scalable, Robust, Micropatterned Silk Fibroin Cell Substrates

Microphysiological Systems as Enabling Tools for Modeling Complexity in the Tumor Microenvironment and Accelerating Cancer Drug Development

Engineered Paper‐Based Cell Culture Platforms

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