[Paper] A CMOS Optoelectronic Neural Interface Device Based on an Image Sensor with On-chip Light Stimulation and Extracellular Neural Signal Recording for Optogenetics

Abstract

184Multifunctional devices have also be proposed to perform optical stimulation and electric neural measurement simultaneously. Optrode is a group of devices consisting of light-guide structure and electrode for electric measurement 7)~9) . In most cases, one or several light-guides are implemented onto an electrode array. Light is deliverd to the optorode using optical fibers. The optical fiber and wire harness prevent to use the optorodes in freely moving situations. A device with capabilities of local light stimulation and electric neural measurement with fexible interconnection is required for freely-moving situations. In this work, we propose a CMOS-based optoelectronic device which is capable of not only stimulating the neural system with integrated micro light source array, Abstract A CMOS-based optoelectronic device is proposed for on-chip neural stimulation and observation with optogenetic methodology. The device is capable of local light delivery for stimulation and electrical neural signal recording. The device consists of an array of InGaN light emitting diodes (LEDs) and Au stacked bump electrodes integrated on a CMOS image sensor. Capabilities of on-chip light stimulation and signal recordingwere quantitatively characterized. We have also confirmed that neuron-like cells can be cultured on the surface of the device.

Optogenetics

“…The typical stimulation intensity used in optogenetics experiments is within 10 mW/mm 2 . The core of the device is a multifunctional CMOS image sensor, which we have developed in previous works (Tokuda et al 2007a(Tokuda et al , b , 2012 Nakajima et al 2012 ;Sawadsaringkarn et al 2013 ). This comparison suggests that if we place a GaInN blue LED near the target neural cells, we can perform optical stimulation of rhodopsin proteins.…”

“Optical communication with brain cells by means of an implanted duplex micro-device with optogenetics and Ca2+ fluoroimaging”

“…Such a technique would be useful for functional brain analysis and could be used to control perception, behavior, and emotion in a freely moving animal. As such, optical neural interface devices have been investigated heretofore 26 27 28 29 30 31 . In the present study, an implantable device incorporating optogenetics and Ca 2+ imaging and its control system was newly developed.…”