Papaverine inhibits lipopolysaccharide-induced microglial activation by suppressing NF-&amp;kappa;B signaling pathway

Dang, Yalong; Mu, Yalin; Wang, Kun; Xu, Ke; Yang, Jing; Zhu, Yu; Luo, Bin

doi:10.2147/dddt.s97380

Cited by 24 publications

(17 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Papaver spp. has various functional benefits that are widely used to treat various issues, such as dental problems [ 10 ], neuronal disorders [ 11 ], cough [ 12 ], pain relief [ 13 ], microbial infections [ 14 ], and cancer [ 15 ]. Some species’ seeds, pedicles, and petals are used in salads or sorbets [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs

Shin

et al. 2018

IJMS

View full text Add to dashboard Cite

The Papaver spp. (Papaver rhoeas (Corn poppy) and Papaver nudicaule (Iceland poppy)) genera are ornamental and medicinal plants that are used for the isolation of alkaloid drugs. In this study, we generated 700 Mb of transcriptome sequences with the PacBio platform. They were assembled into 120,926 contigs, and 1185 (82.2%) of the benchmarking universal single-copy orthologs (BUSCO) core genes were completely present in our assembled transcriptome. Furthermore, using 128 Gb of Illumina sequences, the transcript expression was assessed at three stages of Papaver plant development (30, 60, and 90 days), from which we identified 137 differentially expressed transcripts. Furthermore, three co-occurrence heat maps are generated from 51 different plant genomes along with the Papaver transcriptome, i.e., secondary metabolite biosynthesis, isoquinoline alkaloid biosynthesis (BIA) pathway, and cytochrome. Sixty-nine transcripts in the BIA pathway along with 22 different alkaloids (quantified with LC-QTOF-MS/MS) were mapped into the BIA KEGG map (map00950). Finally, we identified 39 full-length cytochrome transcripts and compared them with other genomes. Collectively, this transcriptome data, along with the expression and quantitative metabolite profiles, provides an initial recording of secondary metabolites and their expression related to Papaver plant development. Moreover, these profiles could help to further detail the functional characterization of the various secondary metabolite biosynthesis and Papaver plant development associated problems.

show abstract

Section: Introductionmentioning

confidence: 99%

Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs

Shin

et al. 2018

IJMS

View full text Add to dashboard Cite

show abstract

“…Neuroinflammation with excessively activated microglia has been demonstrated to aggravate the pathophysiology of many neurodegenerative diseases, indicating the beneficial efficacy of microglia regulatory agents in the therapy of these disorders (23,24). As disclosed in our previous study, total astragalosides alleviated neuroinflammation via the inhibition of iNOS and other inflammatory cytokines in mice with experimental autoimmune encephalomyelitis (25).…”

Section: Discussionmentioning

confidence: 62%

Isoastragaloside I inhibits NF-κB activation and inflammatory responses in BV-2 microglial cells stimulated with lipopolysaccharide

Liu

Huang

et al. 2017

International Journal of Molecular Medicine

View full text Add to dashboard Cite

The excessive activation of microglia in many neurodegenerative diseases is detrimental to neuronal survival. Isoastragaloside I (ISO I) is a natural saponin molecule found within the roots of Astragalus membranaceus, a famous traditional Chinese medicine. In the present study, the anti‑inflammatory effects and the mechanisms of action of ISO I on activated BV-2 cells stimulated with lipopolysaccharide (LPS) were investigated. ISO I dose‑dependently inhibited the excessive release of nitric oxide (NO) and tumor necrosis factor (TNF)-α in the LPS-stimulated BV-2 cells. Moreover, it decreased the production of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), and mitigated the gene expression of interleukin (IL)-1β, TNF-α and iNOS induced by LPS. Further experiments revealed that ISO I decreased the phosphorylation levels of nuclear factor-κB (NF-κB), and suppressed its nuclear translocation and transactivation activity. In addition, it inhibited the activation of signaling pathway molecules, such as PI3K, Akt and mitogen-activated protein kinases (MAPKs). Taken together, our findings suggest that ISO I prevents LPS-induced microglial activation probably by inhibiting the activation of the NF-κB via PI3K/Akt and MAPK signaling pathways, indicating its therapeutic potential for neurological diseases relevant to neuroinflammation.

show abstract

“…A growing body of evidence suggests that inhibition of NF-κB can directly or indirectly promote neuroprotective M2 polarization of the microglia [31,32]. Dang et al [31].…”

Section: Cellular Physiology and Biochemistrymentioning

confidence: 99%

“…A growing body of evidence suggests that inhibition of NF-κB can directly or indirectly promote neuroprotective M2 polarization of the microglia [31,32]. Dang et al [31]. found that papaverine promoted the transformation of activated BV2 cells from M1/M2b to a neuroprotective M2a phenotype through NF-κB signaling pathway.…”

Section: Cellular Physiology and Biochemistrymentioning

confidence: 99%

The Role of Alpha-Lipoic Acid in the Pathomechanism of Acute Ischemic Stroke

Wang¹,

Lv²,

Sun³

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Ischemic stroke results in increased cerebral infarction, neurological deficits and neuroinflammation. The underlying mechanisms involving the anti-inflammatory and neuroprotective properties of α-Lipoic acid (α-LA) remain poorly understood. Herein, we investigated the potential role of α-LA in a middle cerebral artery occlusion (MCAO) rat model and an in vitro lipopolysaccharide (LPS)-induced microglia inflammation model. Methods: In the in vivo study, infarct volume was examined by TTC staining and Garcia score was used to evaluate neurologic recovery. The cytokines were evaluated by enzyme-linked immunosorbent assay, and protein expression of microglia phenotype and NF-κB were measured using western blot. In the in vitro study, the expressions of microglia M1/M2 phenotype were evaluated using qRT-PCR, and immunofluorescence staining was used to assess the nuclear translocation of NF-κB. Results: Both 20 mg/kg and 40 mg/kg of α-LA alleviated infarct size, brain edema, and neurological deficits. Furthermore, α-LA induced the polarization of microglia to the M2 phenotype, modulated the expression of IL-1β, IL-6, TNF-α and IL-10, and attenuated the activation of NF-κB after MCAO. α-LA inhibited the expression of M1 markers, increased activation of the M2 markers, and suppressed the nuclear translocation of NF-κB in LPS-stimulated BV2 microglia. Conclusions: α-LA improved neurological outcome in experimental stroke via modulating microglia M1/M2 polarization. The potential mechanism of α-LA might be mediated by inhibition of NF-κB activation via regulating phosphorylation and nuclear translocation of p65.

show abstract

Papaverine inhibits lipopolysaccharide-induced microglial activation by suppressing NF-κB signaling pathway

Cited by 24 publications

References 31 publications

Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs

Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs

Isoastragaloside I inhibits NF-κB activation and inflammatory responses in BV-2 microglial cells stimulated with lipopolysaccharide

The Role of Alpha-Lipoic Acid in the Pathomechanism of Acute Ischemic Stroke

Contact Info

Product

Resources

About