Papaverine Biotransformation in Plant Cell Suspension Cultures

Biotransformation of terpenes and related compounds by suspension culture ofGlycyrrhiza glabra L. (Papilionaceae)

“…13 Therefore we examined the chemical modifications of exogenously supplied terpenes and related substrates (Table 1). Aldehydes with a saturated [octanal (4), isovaleraldehyde] and an unsaturated [perilla aldehyde (7), cinnarnaldehyde (8) (α-β unsaturated), citral or citronellal (2)] structures or possessing aromatic ring systems [4-anisaldehyde (1), 2-anisaldehyde, benzaldehyde (5) and vanillin], ketones with an α-β unsaturated [carvone (3)] or saturated (menthone) structures, an ester [bornyl acetate (6)], a phenol (carvacrol), alcohols (linalol and menthol) or hydrocarbons (p-cymene and γ-terpinene) were chosen for the biotransformation. Using these compounds we examined the biotransformation of terpenoids in cultured cells of G. glabra to elucidate the metabolic behavior of the cells at a concentration of ca.…”

“…Some of them deal with the production of glycyrrhetinic acid and glycyrrhizin, 4,5 or biotransformation of 18 β-glycyrrhetinic acid and papaverine. [6][7][8] No report has described the biotransformation of terpenoids by G. glabra. In an attempt to examine variation in the biotransformation of different classes of terpenoids and related compounds, we chose cell suspension cultures of G. glabra var.…”

Centella asiatica (L.) Urban, (Pennywort): Cell Culture, Production of Terpenoids, and Biotransformation Capacity

“…Thus, papaverine was converted to its oxidized derivative, papaveraldine, by a cell suspension culture of C. asiatica. Several other studies have reported biotransformation of papaverine into papaveraldine by plant cell culture: Silene alba, Cardamine pratensis, Digitalis purpurea (Bister-Miel et al 1988), Vinca minor (Podvin 1985), Cinchona succirubra, Ptelea trifoliata, Phaseolus vulgaris, Parthenocissus tricuspidata (Rideau et al 1988), and Glycyrrhiza glabra (Dorisse et al 1988). (Solet 1993) Cell suspension cultures of C. asiatica of Professor Guignard's laboratory have also shown capacity of thiocolchicine demethylation and glucosylation (Fig.…”

Glycyrrhiza glabra (Linn.) and Lavandula officinalis (L.) cell suspension cultures-based biotransformation of β-artemether

“…Plant cell suspension cultures and cell-free enzyme extracts have been frequently employed as efficient biocatalysts to carry out complex regiospecific or stereospecific chemical reactions that are otherwise difficult or very costly to perform through synthetic mechanisms [23]. Cultured cells of G. glabra have earlier been used to biotransform papaverine, carvone, bornyl acetate, 18b-glycyrrhetinic acid, terpenes, and related compounds by many investigators [24][25][26], but the use of cell cultures of L. officinalis in biotransformation work is confined to only two investigations wherein their efficacy to either reducing monoterpenoid aldehydes into their corresponding primary alcohols [27] or for the deoxygenation of artemisinin [20] was reported. The present study constitutes the first report on the successful utilization of cell cultures of these two plant systems in the bioconversion of anti-malarial compound b-artemether into its THF-acetate derivative.…”