Papain gel containing methylene blue for simultaneous caries removal and antimicrobial photoinactivation against Streptococcus mutans biofilms

Silva, Zenildo Santos; Huang, Ying‐Ying; Freitas, Lucas Freitas de; França, Cristiane Miranda; Botta, Sérgio Brossi; Ana, Patrícia Aparecida; Mesquita‐Ferrari, Raquel Agnelli; Fernandes, Kristianne Porta Santos; Deana, Alessandro Melo; Leal, Cintia Raquel Lima; Prates, Renato Araújo; Hamblin, Michael R.; Bussadori, Sandra Kalil

doi:10.1038/srep33270

Cited by 30 publications

(12 citation statements)

References 37 publications

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“…Papain, with its proteolytic activity against the amino acid, has been extensively used in the fields of medicine and food [90]. Papain-containing materials have shown successful results when used against S. mutans biofilm [89,91]. M. alternifolia is known as “tea tree”.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Activities of Herbal Toothpastes Combined with Essential Oils against Streptococcus mutans

et al. 2019

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In recent years, people have become more conscious about the side-effects of fluoride toothpastes and herbal products have drawn attention as alternatives in the struggle against caries. Studies have focused on the benefits of essential oils obtained from herbs because of their antibacterial effects. The aim of this study was to evaluate and compare the antibacterial activity of Origanum dubium and Cinnamomum cassia oils combined with herbal toothpastes against Streptococcus mutans. The antibacterial activity of the test materials was determined using the agar well diffusion method before and after the addition of essential oils. We tested the efficacy of Splat Organic and Splat Biocalcium against S. mutans (12 mm and 11 mm, respectively) doubled in combination with Origanum dubium (23 mm for both toothpastes) and tripled with Cinnamomum cassia (38 mm and 36 mm, respectively). Jack N’ Jill toothpaste, which did not initially show any antibacterial effect, exhibited the largest inhibition zones after the addition of the essential oils (38 mm for Origanum dubium and 39 mm for Cinnamomum cassia). The results of this study pointed out that herbal toothpastes exhibit statistically higher antibacterial activity against Streptococcus mutans (p < 0.05) than their initial forms after the addition of essential oils.

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Section: Discussionmentioning

confidence: 99%

Antibacterial Activities of Herbal Toothpastes Combined with Essential Oils against Streptococcus mutans

et al. 2019

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“…1) 51 . The dye molecule can also act as a photosensitizer [52][53][54] and it was adsorbed on catalyst surface during the oxidation-reduction experiment (Eqs. 2 and 3).…”

Section: Resultsmentioning

confidence: 99%

One-step Synthesized Silver Nanoparticles Using Isoimperatorin: Evaluation of Photocatalytic, and Electrochemical Activities

Mavaei

Chahardoli

Shokoohinia

et al. 2020

Sci Rep

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in the current study, isoimperatorin, a natural furanocoumarin, is used as a reducing reagent to synthesize isoimperatorin mediated silver nanoparticles (iso-Agnps), and photocatalytic and electrocatalytic activities of iso-Agnps are evaluated. iso-Agnps consisted of spherically shaped particles with a size range of 79-200 nm and showed catalytic activity for the degradation (in high yields) of New Fuchsine (NF), Methylene Blue (MB), Erythrosine B (ER) and 4-chlorophenol (4-CP) under sunlight irradiation. Based on obtained results, Iso-AgNPs exhibited 96.5%, 96.0%, 92%, and 95% degradation rates for MB, NF, ER, and 4-CP, respectively. The electrochemical performance showed that the as-prepared iso-Agnps exhibited excellent electrocatalytic activity toward hydrogen peroxide (H 2 o 2) reduction. it is worth noticing that the iso-Agnps were used as electrode materials without any binder. The sensor-based on binder-free Iso-AgNPs showed linearity from 0.1 µM to 4 mM with a detection limit of 0.036 μM for H 2 o 2. this binder-free and straightforward strategy for electrode preparation by silver nanoparticles may provide an alternative technique for the development of other nanomaterials based on isoimperatorin under green conditions. Altogether, the application of isoimpratorin in the synthesis of nano-metallic electro and photocatalysts, especially silver nanoparticles, is a simple, cost-effective and efficient approach. Recently, isolated and purified natural products have gained considerable attention in the synthesis and cap of metallic nanoparticles. These natural product-induced metallic nanoparticles (NPIMNPs) have been widely used in the biological, environmental, electrochemical, and catalytic fields 1-8. Albeit plant extracts are considered as general sources for the green synthesis of metallic nanoparticles 9 , the extracts compositions and concentrations of substances are highly variable, affected by the method of extraction, harvesting season and geographical parameters. As an alternative, the pure natural products with a well-defined concentration of reactant can synthesize reproducible metallic nanoparticles, and the purification of nanoparticles is more facile 10,11. Furthermore, physicochemical and biological characteristics of natural products can improve the biological, electrochemical, and catalytic properties of NPIMNPs. In this regard, natural products e.g., phenolic compounds, alkaloids, terpenoids, amino acids, flavonoids, glutathiones, quinones antioxidants, polysaccharides, organic acids, and coumarins have been used 12. The synthesis of nanoparticles by these natural products is a clean, non-toxic, and environmental-friendly 13. Compared to their counterparts, synthesized by other methods, NPIMNPs possess improved bioactivities and catalytic characteristics 12. Among metallic nanoparticles, silver nanoparticles have been widely studied for catalytic activities. AgNPs can demonstrate light absorption and significant visible light catalytic activity due to its narrow bandgap energy

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“…In Dentistry, the most widely used PSs are methylene blue (MB) and toluidine blue (TB) [8,9], which are capable of interacting with the cell membrane, inactivating gram-positive and gram-negative bacteria, acting mainly by damaging the cytoplasmic membrane and DNA, and may reach multiple cellular targets [7], which hinders the appearance of resistant microorganisms [10].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue

Catão

Batista

2020

Pesqui. Bras. Odontopediatria Clín. Integr.

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Papain gel containing methylene blue for simultaneous caries removal and antimicrobial photoinactivation against Streptococcus mutans biofilms

Cited by 30 publications

References 37 publications

Antibacterial Activities of Herbal Toothpastes Combined with Essential Oils against Streptococcus mutans

Antibacterial Activities of Herbal Toothpastes Combined with Essential Oils against Streptococcus mutans

One-step Synthesized Silver Nanoparticles Using Isoimperatorin: Evaluation of Photocatalytic, and Electrochemical Activities

In Vitro Evaluation of the Antibacterial Effect of Photodynamic Therapy with Methylene Blue

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