Panel VII: Neutrophil concentrates: Functional considerations, storage, and quality control

Glasser, Leslie; Lane, Thomas A.; McCullough, Jeffrey; Price, Thomas H.

doi:10.1002/jca.2920010309

Cited by 21 publications

(10 citation statements)

References 38 publications

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“…It is known that an important parameter that impairs polymorphonuclear leukocyte functions is the decrease of pH below 7.0 during storage (2)(3)(4)10). That is why the addition of 15 mmol/1 bicarbonate final concentration to all units of granulocyte concentrate was recominended by Lane & Lamkin in 1984 (10).…”

Section: H Preservation (Assays I To Xi)mentioning

confidence: 99%

“…Polymorphonuclear leukocytes were stored at room temperature, under several different conditions (2)(3)(4). The temperature was accurately recorded for each assay.…”

Section: Storage Proceduresmentioning

confidence: 99%

“…« r , . ' f optimal storage is achieved at a pH close to 7.4, at would allow an eas,er m vitro study of their struc ure ^ ature (20 _ 24 OQ ^ a^tati and and functions, an ea 5 ier study ^polynoiphoBDckar ^ ^ ^ supplementation (2)(3)(4). Sevleukocyte diseases (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Liquid Preservation of Polymorphonuclear Leukocytes: Effect of Various Additives on Chemotaxis Preservation

Verstraeten¹,

Marchand-Arvier²,

Schooneman³

et al. 1991

Clinical Chemistry and Laboratory Medicine

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Adequate storage of polymorphonuclear leukocytes would allow an easier in vitro study of their structure and their functions, an easier study of polymorphonuclear leukocyte diseases (e. g. chronic granulomatous disease) and an easier use of polymorphonuclear leukocytes äs a clinical tool (e. g. for localizing infections). Unfortunately, polymorphonuclear leukocytes are nearly impossible to preserve, even in shortterm storage. This study proposes a model for the study of polymorphonuclear leukocyte storage in a synthetic medium: Plasmion®.For storage over a period of 24 h, we found that supplementation with each of the following additives: bicarbonate buffer, glucose, adenosine triphosphate (ATP), ascorbic acid, nicotinamide adenine dinucleotide (NADH), nicotinamide adenine dinucleotide phosphate (NADPH), oc-tocopherol acetate, amikacin and ampicillin, significantly improves (p < 0.05) one or several functions of the polymorphonuclear leukocytes.When samples were stored for 48 h, we found that the addition of bicarbonate buffer after 24 h significantly improves the maintenänce of several functions of polymorphonuclear leukocytes, in particular chemotaxis.Preservation for 96 h was achieved by making additions of Supplements on each day of storage, with a chemotaxis maintenänce of 83% at 24 h, 59% at 48 h, 46% at 24 h and 20% at 96 h.In conclusion, by using the Plasmion® medium, and adding the above-mentioned compounds on each day of storage, chemotaxis can be satisfactorily maintained over 4 day s.

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Section: H Preservation (Assays I To Xi)mentioning

confidence: 99%

“…Polymorphonuclear leukocytes were stored at room temperature, under several different conditions (2)(3)(4). The temperature was accurately recorded for each assay.…”

Section: Storage Proceduresmentioning

confidence: 99%

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Liquid Preservation of Polymorphonuclear Leukocytes: Effect of Various Additives on Chemotaxis Preservation

Verstraeten¹,

Marchand-Arvier²,

Schooneman³

et al. 1991

Clinical Chemistry and Laboratory Medicine

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“…This is consistent with data from other laboratories. 14,15 These cells were determined to be viable or healthy neutrophils by Trypan blue exclusion before CL measurement. 16,17 Twentyfive cuvette positions were available in the luminometer and were set up with variables in triplicate or duplicate.…”

Section: Chemiluminescence Assaymentioning

confidence: 99%

Effect of cellulose acetate materials on the oxidative burst of human neutrophils

Moore

Kaplan

Picciolo

et al. 2001

J. Biomed. Mater. Res.

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Following adverse clinical events involving seven patients undergoing renal dialysis using 12-year-old cellulose acetate hemodialyzers, this in vitro study was proposed in an effort to characterize the inflammatory response to the constituent cellulose acetate (CA) fiber materials. Chemiluminescence (CL) and apoptosis assays were used to determine whether human neutrophils were activated by CA fiber materials and/or are sensitive to degradation/alteration of these fibers over time. Furthermore, the study examined in vitro assays with human neutrophils using a CA film, the solvents used in the film preparation and CA resin. The film could be cut to identical sized pieces in an effort to compare hemodialysis material effects in standardized amounts. For the CL assays, 60-min exposure was followed by secondary stimulation with n-formyl-met-leu-phe (fMLP) or phorbol-12-myristate-13-acetate (PMA). Short-term exposure (60-min postintroduction to CA materials) increased the inflammatory response as measured by the respiratory burst of neutrophils (p < or =.05), with CA fiber exposure significantly compared with cells alone. There was a trend toward an increased response with exposure to older fibers with secondary PMA stimulation. Apoptosis was increased 12% with exposure to the more aged fibers versus 2% with the new fibers. The fiber storage component, glycerol, significantly inhibited the oxidative response (p < or =.001; > or =80% suppression with concentrations of 5-20%). The solvents used in film preparation, N,N-dimethylacetamide and tetrahydrofuran, produced greater than a 70% and 60% suppression, respectively, of CL activity for all concentrations > or =1%. More work is needed to determine the specific nature of the interaction of inflammatory cells with CA materials, but early evidence suggests that neutrophils are activated by CA and display an altered response to more aged fibers.

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“…This procedure is severely hampered by the need for repeated transfusions and the inability to store the large number of cells needed. [5][6][7] The recently characterized common myeloid progenitors (CMPs) and granulocyte-monocyte progenitors (GMPs) are subpopulations of progenitor cells that are committed to the myeloid lineages. 8 The biology of CMPs and GMPs predicts that infusion of these cells would be beneficial in the amelioration of neutropenia.…”

Section: Introductionmentioning

confidence: 99%

Single infusion of myeloid progenitors reduces death from Aspergillus fumigatus following chemotherapy-induced neutropenia

BitMansour

Cao

Chao

et al. 2005

Blood

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Hematopoietic progenitors committed to the myeloid lineage, the common myeloid and granulocyte-monocyte progenitors (CMP/GMP), have been shown to protect against opportunistic pathogens following myeloablative radiation; however, the efficacy of this approach has not been studied in the setting of chemotherapyinduced neutropenia. In this mouse model, the infusion of CMP/GMP on the day after 5-fluorouracil (5-FU) administration (D؉1) resulted in a significant increase in the number of splenic neutrophils by D؉8 when compared with 5-FUonly controls (P ‫؍‬ .02), the majority of which were CMP/GMP-derived (54%). Moreover, 19% and 28% of neutrophils in the blood and bone marrow, respectively, were CMP/GMP-derived. Survival following intranasal challenge with the fungus Aspergillus fumigatus was significantly higher in CMP/GMP-infused mice than the controls (56% and 33% respectively; P ‫؍‬ .019). Thus, a single infusion of CMP/ GMP enhances tissue neutrophil content and increases survival against a lethal challenge with A fumigatus in the setting of chemotherapy-induced neutropenia.

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Panel VII: Neutrophil concentrates: Functional considerations, storage, and quality control

Cited by 21 publications

References 38 publications

Liquid Preservation of Polymorphonuclear Leukocytes: Effect of Various Additives on Chemotaxis Preservation

Liquid Preservation of Polymorphonuclear Leukocytes: Effect of Various Additives on Chemotaxis Preservation

Effect of cellulose acetate materials on the oxidative burst of human neutrophils

Single infusion of myeloid progenitors reduces death from Aspergillus fumigatus following chemotherapy-induced neutropenia

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