Pandoraea pulmonicola chronic colonization in a cystic fibrosis patient, France

Kokcha, Sahare; Bittar, Fadi; Reynaud-Gaubert, M.; Mély, Laurent; Gomez, Carine; Gaubert, J.; Thomas, Philippe; Rolain, Jean‐Marc

doi:10.1002/2052-2975.16

Cited by 13 publications

(17 citation statements)

References 12 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the last decade, many emerging multidrug resistant pathogens have been described in CF patients including new species of the Burkholderia cepacia complex, Brevundimonas diminuta , Inquilinus limosus , Acetobacter indonesiensis , Achromobacter xylosoxidans , Ochrobactrum anthropi , and bacteria of the genus Pandoraea [ 17 , 18 ]. The pathogenicity of Pandoraea species remains controversial.…”

Section: Discussionmentioning

confidence: 99%

First time identification of Pandoraea sputorum from a patient with cystic fibrosis in Argentina: a case report

Martina

Martínez

et al. 2017

BMC Pulm Med

View full text Add to dashboard Cite

Background Pandoraea species are considered emerging pathogens in the context of cystic fibrosis (CF) and are difficult to identify by conventional biochemical methods. These multidrug resistant bacteria remain poorly understood particularly in terms of natural resistance, mechanisms of acquired resistance and impact on the prognosis of the disease and the lung function. Among them, Pandoraea sputorum has been previously described in few cases of CF patients from Spain, Australia, France and United States, underlining the need of more clinical data for a better knowledge of its pathogenicity. This is the first report relating to P. sputorum in a CF patient in Argentina.Case presentation Pandoraea sputorum was identified in a nine-year-old cystic fibrosis patient from Argentina, after treatment failure during an exacerbation. The isolates were successfully identified by combining molecular techniques based on 16S rRNA sequencing and mass spectrometry (MS) methods, after reassessing previous misidentified isolates by conventional methods. After first isolation of P. sputorum, patient’s clinical condition worsened but later improved after a change in the treatment. Although isolates showed susceptibility to trimethoprim–sulfamethoxazole and imipenem, in our case, the antibiotic treatment failed in the eradication of P. sputorum.ConclusionsAll combined data showed a chronic colonization with P. sputorum associated to a deterioration of lung function. We noted that the presence of P. sputorum can be underestimated in CF patients and MALDI-TOF MS appears to be a promising means of accurate identification of Pandoraea species.

show abstract

Section: Discussionmentioning

confidence: 99%

First time identification of Pandoraea sputorum from a patient with cystic fibrosis in Argentina: a case report

Martina

Martínez

et al. 2017

BMC Pulm Med

View full text Add to dashboard Cite

show abstract

“…However, identification remains challenging in routine practice because mass spectrometry databases are incomplete and molecular identification may still be required for accurate knowledge on epidemiology and clinical implications of Pandoraea ( Fernández-Olmos et al, 2012 ). In France, the first human case involving P. pulmonicola was reported in 2013 in a CF patient chronically colonized who died of P. aeruginosa sepsis ( Kokcha et al, 2013 ). The epidemic spread of P. pulmonicola was also firstly described among six CF patients resulting in chronic colonization in the six patients who were also chronically colonized by P. aeruginosa ( Degand et al, 2015 ); among them, three patients died, two patients remained clinically stable, and the remaining patient had a decline in lung function, as observed for the patient of the present study in whom dynamics of P. pulmonicola colonization may have been driven by antipseudomonal therapies ( Kokcha et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

“…In France, the first human case involving P. pulmonicola was reported in 2013 in a CF patient chronically colonized who died of P. aeruginosa sepsis ( Kokcha et al, 2013 ). The epidemic spread of P. pulmonicola was also firstly described among six CF patients resulting in chronic colonization in the six patients who were also chronically colonized by P. aeruginosa ( Degand et al, 2015 ); among them, three patients died, two patients remained clinically stable, and the remaining patient had a decline in lung function, as observed for the patient of the present study in whom dynamics of P. pulmonicola colonization may have been driven by antipseudomonal therapies ( Kokcha et al, 2013 ). Regarding virulence, our results corroborated previous results suggesting that biofilm formation is not a major virulence factor in this genus ( Caraher et al, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

“…After first colonization, Pandoraea spp. were able to chronically colonize the CF respiratory tract (CFRT) ( Fernández-Olmos et al, 2012 ; Kokcha et al, 2013 ; Pugès et al, 2015 ; Martina et al, 2017 ), were transmissible between patients ( Jørgensen et al, 2003 ; Degand et al, 2015 ) and can produce severe lung diseases and bacteremia ( Pimentel and MacLeod, 2008 ; Kokcha et al, 2013 ). The pathogenicity appears mainly supported by a pro-inflammatory response induction significantly greater than with Pseudomonas aeruginosa ( Caraher et al, 2008 ; Costello et al, 2014 ) and the treatment may be complicated by multidrug resistance conferred by carbapenem-hydrolyzing oxacillinases ( Caraher et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Highly Diversified Pandoraea pulmonicola Population during Chronic Colonization in Cystic Fibrosis

et al. 2017

View full text Add to dashboard Cite

Several environmental bacteria are considered as opportunistic pathogens in cystic fibrosis (CF) and are able to persistently colonize the CF respiratory tract (CFRT). Beside Pseudomonas aeruginosa and Burkholderia cepacia complex, Pandoraea spp. are defined as pathogenic. During chronic colonization, adaptive evolution and diversified population have been demonstrated, notably for P. aeruginosa. However, the persistence of Pandoraea in the CFRT remains largely unexplored. We studied genomic and phenotypic traits of Pandoraea pulmonicola isolates successively recovered from the airways of a single CF patient and relate the results to qualitative and quantitative evolution of other cultivable pathogens and to patient clinical status. A total of 31 isolates recovered from 18 sputum samples over a 7-year period in a single CF patient were studied. Genome dynamics was assessed by pulsed-field gel electrophoresis, ERIC-PCR fingerprinting and 16S rRNA gene PCR-temporal temperature gel electrophoresis. Phenotypic features included antimicrobial susceptibility, motility, biofilm production, and virulence in Caenorhabditis elegans model. Variability was observed for all the characteristics studied leading to highly diversified patterns (24 patterns) for the 31 clonally related isolates. Some of these modifications, mainly genomic events were concomitantly observed with CFRT microbiota composition shifts and with severe exacerbations. The diversity of P. pulmonicola population studied, observed for isolates recovered from successive samples but also within a sample suggested that existence of a diversified population may represent a patho-adaptive strategy for host persistence in the heterogeneous and fluctuating CFRT environment.

show abstract

“…Multiple case reports published on Pandoraea spp. identify them as some of the pathogens responsible for chronic lung infections alongside the infamous Pseudomonas aeruginosa ( 2 – 5 ). The severity of infection by Pandoraea spp.…”

Section: Genome Announcementmentioning

confidence: 99%

Pandoraea sp. Strain E26: Discovery of Its Quorum-Sensing Properties via Whole-Genome Sequence Analysis

et al. 2015

View full text Add to dashboard Cite

show abstract

Pandoraea pulmonicola chronic colonization in a cystic fibrosis patient, France

Cited by 13 publications

References 12 publications

First time identification of Pandoraea sputorum from a patient with cystic fibrosis in Argentina: a case report

First time identification of Pandoraea sputorum from a patient with cystic fibrosis in Argentina: a case report

Highly Diversified Pandoraea pulmonicola Population during Chronic Colonization in Cystic Fibrosis

Pandoraea sp. Strain E26: Discovery of Its Quorum-Sensing Properties via Whole-Genome Sequence Analysis

Contact Info

Product

Resources

About