Pancreatic triglyceride lipase is involved in the virulence of the brown planthopper to rice plants

Yuan, Longyu; Hao, Yuanhao; Chen, Qiao-kui; Pang, Rui; Zhang, Wenqing

doi:10.1016/s2095-3119(20)63188-4

Cited by 7 publications

(5 citation statements)

References 29 publications

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“…Compared to mammals, few studies on insect pancreatic TAG-lipases exist, such as a pancreatic lipase-related protein as found in the brown planthopper, Nilaparvata lugens , that is essential for the hatching of eggs [ 57 , 58 ]. A functional study using RNAi found that pancreatic TAG-lipases may be related to the virulence of BPH to rice varieties [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Proteomics and ultrastructural analysis of Hermetia illucens (Diptera: Stratiomyidae) larval peritrophic matrix

et al. 2021

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Background The black soldier fly (Hermetia illucens) has significant economic potential. The larvae can be used in financially viable waste management systems, as they are voracious feeders able to efficiently convert low-quality waste into valuable biomass. However, most studies on H. illucens in recent decades have focused on optimizing their breeding and bioconversion conditions, while information on their biology is limited. Methods About 200 fifth instar well-fed larvae were sacrificed in this work. The liquid chromatography-tandem mass spectrometry and scanning electron microscopy were employed in this study to perform a proteomic and ultrastructural analysis of the peritrophic matrix (PM) of H. illucens larvae. Results A total of 565 proteins were identified in the PM samples of H. illucen, of which 177 proteins were predicted to contain signal peptides, bioinformatics analysis and manual curation determined 88 proteins may be associated with the PM, with functions in digestion, immunity, PM modulation, and others. The ultrastructure of the H. illucens larval PM observed by scanning electron microscopy shows a unique diamond-shaped chitin grid texture. Conclusions It is the first and most comprehensive proteomics research about the PM of H. illucens larvae to date. All the proteins identified in this work has been discussed in details, except several unnamed or uncharacterized proteins, which should not be ignored and need further study. A comparison of the ultrastructure between H. illucens larval PM and those of other insects as observed by SEM indicates that the PM displays diverse textures on an ultra-micro scale and we suscept a unique diamond-shaped chitin grid texture may help H. illucens larval to hold more food. This work deepens our understanding of the molecular architecture and ultrastructure of the H. illucens larval PM.

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Section: Discussionmentioning

confidence: 99%

Proteomics and ultrastructural analysis of Hermetia illucens (Diptera: Stratiomyidae) larval peritrophic matrix

et al. 2021

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“…To determine the effect of NlSP7 on BPH feeding, we used the EPG technique to profile the feeding behavior of piercing–sucking insects 19 . Five main feeding phases can be distinguished by EPG: (1) the non-penetration phase, (2) the pathway phase (including penetration, salivation, stylet movement, and extracellular activity near the phloem), (3) the intracellular phase of activity in the phloem, (4) the phloem sap ingestion phase, and (5) the xylem phase.…”

Section: Resultsmentioning

confidence: 99%

Salivary protein 7 of the brown planthopper functions as an effector for mediating tricin metabolism in rice plants

Gong

Yuan

et al. 2022

Sci Rep

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The brown planthopper (BPH), Nilaparvata lugens, is an important pest that affects rice (Oryza sativa) production in Asia. The flavone tricin (5,7,4′-trihydroxy-3′,5′-dimethoxy flavone) is a valuable secondary metabolite commonly found in rice plants that can defend rice plants against infestation by BPH. BPH damage can reduce the metabolic level of tricin in rice. Our preliminary transcriptome research results showed that BPH salivary protein 7 (NlSP7), is highly responsive to tricin stimuli. However, the function of NlSP7 in mediating the interaction between the rice plant and the BPH is unknown. In this study, we cloned the NlSP7 gene in N. lugens and found that its mRNA level was greater in the presence of high tricin content than low tricin content, regardless of whether the BPHs were fed a rice plant diet or an artificial diet containing 100 mg/L tricin. Knocking down NlSP7 resulted in BPH individuals spending more time in the non-penetration and pathway phase, and less time feeding on the phloem of rice plants. These changes decreased BPH food intake, feeding behavior, and fitness, as well as the tricin content of the rice plants. These findings demonstrate that the salivary protein 7 of BPH functions as an effector for tricin metabolism in rice.

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“…To determine the effect of NlSP7 on BPH feeding, we used the EPG technique to pro le the feeding behavior of piercing-sucking insects (Yuan et al 2020). Five main feeding phases can be distinguished by EPG: 1) the non-penetration phase, 2) the pathway phase (including penetration, salivation, stylet movement, and extracellular activity near the phloem), 3) the intracellular phase of activity in the phloem, 4) the phloem sap ingestion phase, and 5) the xylem phase.…”

Section: Impairment Of Bph Feedingmentioning

confidence: 99%

Salivary Protein NlSP7 of The Brown Planthopper Functions As An Effector for Mediating Tricin Metabolism in Rice Plants

Gong

Yuan

et al. 2021

Preprint

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The brown planthopper (BPH), Nilaparvata lugens, is an important pest that affects rice (Oryza sativa) production in Asia. The flavone tricin (5,7,4ʹ-trihydroxy-3ʹ,5ʹ-dimethoxy flavone) is a valuable secondary metabolite commonly found in rice plants that can defend rice plants against infestation by BPH. BPH damage can reduce the metabolic level of tricin in rice. Our preliminary transcriptome research results showed that BPH salivary protein 7, NlSP7, is highly responsive to tricin stimuli. However, the function of NlSP7 in mediating the interaction between the rice plant and the BPH is unknown. In this study, we cloned the NlSP7 gene in N. lugens and found that its mRNA level was greater in the presence of high tricin content than low tricin content, regardless of whether the BPHs were fed a rice plant diet or an artificial diet containing 100 mg/L tricin. Knocking down NlSP7 resulted in BPH individuals spending more time in the non-penetration and pathway phase, and less time feeding on the phloem of rice plants. These changes decreased BPH food intake, feeding behavior, and fitness, as well as the tricin content of the rice plants. These findings demonstrate that the salivary protein NlSP7 of BPH functions as an effector for tricin metabolism in rice.

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Pancreatic triglyceride lipase is involved in the virulence of the brown planthopper to rice plants

Cited by 7 publications

References 29 publications

Proteomics and ultrastructural analysis of Hermetia illucens (Diptera: Stratiomyidae) larval peritrophic matrix

Proteomics and ultrastructural analysis of Hermetia illucens (Diptera: Stratiomyidae) larval peritrophic matrix

Salivary protein 7 of the brown planthopper functions as an effector for mediating tricin metabolism in rice plants

Salivary Protein NlSP7 of The Brown Planthopper Functions As An Effector for Mediating Tricin Metabolism in Rice Plants

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