Pancreatic ribonuclease

Schomburg, Dietmar; Salzmann, Margit

doi:10.1007/978-3-642-76463-9_191

Cited by 2 publications

(1 citation statement)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After treatment with RNase A and heating to 55 °C to bring the DNA droplets to the liquid state, the DNA droplets undergo segregation in the entire observation chamber (Figure 1d). Despite activity of RNase A within a broad temperature range from 15-70 °C,[27] enzymatic activity cannot be spatially controlled in the bulk sample. Photocleavage, instead, could be triggered locally by illumination with the 405nm laser of the confocal microscope.…”

Section: Resultsmentioning

confidence: 99%

A DNA segregation module for synthetic cells

Tran

Chatterjee

Dreher

et al. 2022

Preprint

View full text Add to dashboard Cite

The bottom-up construction of an artificial cell requires the realization of synthetic cell division. Significant progress has been made towards reliable compartment division, yet mechanisms to segregate the DNA-encoded informational content are still in their infancy. Herein, droplets of DNA Y-motifs are formed by liquid-liquid phase separation (LLPS). Entropy-driven DNA droplet segregation is obtained by cleaving the linking component between two populations of DNA Y-motifs. In addition to enzymatic cleavage, photolabile sites are introduced for spatio-temporally controlled DNA segregation in bulk as well as in cell-sized water-in-oil droplets and giant unilamellar lipid vesicles (GUVs). Notably, the segregation process is slower in confinement than in bulk. The ionic strength of the solution and the nucleobase sequences are employed to regulate the segregation dynamics. The experimental results are corroborated in a lattice-based theoretical model which mimics the interactions between the DNA Y-motif populations. Altogether, engineered DNA droplets, reconstituted in GUVs, could represent a strategy towards an entropy-driven DNA segregation module within bottom-up assembled synthetic cells.Table of ContentsAn entropy-driven DNA segregation module for bottom-up assembled synthetic cells is realized. It is based on DNA droplets that are engineered to segregate upon enzymatic or photocleavage inside giant unilamellar lipid vesicles (GUVs). The segregation kinetics is altered by the confinement, as confirmed by lattice-based numerical simulations. DNA segregation is further controlled by temperature, ionic strengths and nucleobase sequence.

show abstract

Section: Resultsmentioning

confidence: 99%

A DNA segregation module for synthetic cells

Tran

Chatterjee

Dreher

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

A DNA Segregation Module for Synthetic Cells

Tran

Chatterjee

Dreher

et al. 2022

Small

View full text Add to dashboard Cite

The bottom‐up construction of an artificial cell requires the realization of synthetic cell division. Significant progress has been made toward reliable compartment division, yet mechanisms to segregate the DNA‐encoded informational content are still in their infancy. Herein, droplets of DNA Y‐motifs are formed by liquid–liquid phase separation. DNA droplet segregation is obtained by cleaving the linking component between two populations of DNA Y‐motifs. In addition to enzymatic cleavage, photolabile sites are introduced for spatio‐temporally controlled DNA segregation in bulk as well as in cell‐sized water‐in‐oil droplets and giant unilamellar lipid vesicles (GUVs). Notably, the segregation process is slower in confinement than in bulk. The ionic strength of the solution and the nucleobase sequences are employed to regulate the segregation dynamics. The experimental results are corroborated in a lattice‐based theoretical model which mimics the interactions between the DNA Y‐motif populations. Altogether, engineered DNA droplets, reconstituted in GUVs, can represent a strategy toward a DNA segregation module within bottom‐up assembled synthetic cells.

show abstract

Pancreatic ribonuclease

Cited by 2 publications

References 27 publications

A DNA segregation module for synthetic cells

A DNA segregation module for synthetic cells

A DNA Segregation Module for Synthetic Cells

Contact Info

Product

Resources

About