Pancreatic proteases in serum induce leukocyte-endothelial adhesion and pancreatic microcirculatory failure

Keck, Tobias; Friebe, V.; Warshaw, Andrew L.; Antoniu, Bozena; Waneck, Gerald L.; Benz, Stefan; Hopt, Ulrich T.; Fernández‐del‐Castillo, Carlos

doi:10.1159/000085278

Cited by 37 publications

(28 citation statements)

References 68 publications

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“…In the second phase, there is intrapancreatic inflammation through a variety of mechanisms and pathways (16,(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28). In the third phase, there is extrapancreatic inflammation including acute respiratory syndrome (ARDS) (16,(19)(20)(21)29).…”

Section: Pathophysiologymentioning

confidence: 99%

Practice Guidelines in Acute Pancreatitis

Banks

Freeman

2006

Am J Gastroenterology

1,662

980

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Section: Pathophysiologymentioning

confidence: 99%

Practice Guidelines in Acute Pancreatitis

Banks

Freeman

2006

Am J Gastroenterology

1,662

980

View full text Add to dashboard Cite

“…3 Pancreatic elastase-1 contributes to the ongoing inflammatory state during acute pancreatitis by enhancing neutrophil-mediated tissue injury, and has been shown experimentally to induce pancreatic microcirculatory failure in the presence of sera co-factors. 7 There have been inconclusive assessments about the clinical utility of serum PE-1 in human beings for diagnosing acute pancreatitis. 1,10,19 In chronic pancreatitis, the exocrine function of the pancreas may become diminished, and in human patients with chronic pancreatitis, serum and fecal PE-1 is often reduced, reflecting subclinical exocrine pancreatic insufficiency.…”

Section: Introductionmentioning

confidence: 99%

Specificity and sensitivity of serum canine pancreatic elastase-1 concentration in the diagnosis of pancreatitis

2011

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Abstract. The aim of the current study was to determine the sensitivity and specificity of serum canine pancreatic elastase-1 (cPE-1) for the diagnosis of pancreatitis in dogs. The study was prospective, assessing dogs presenting with clinical signs similar to pancreatitis. Sixty-one dogs were recruited (49 with pancreatic disease and 12 with non-pancreatic disease). There was no significant difference in serum cPE-1 between dogs with pancreatic disease and non-pancreatic disease. However, there was a significant difference in serum cPE-1 between severe acute pancreatitis and non-pancreatic disease. A cut-off value for serum cPE-1 > 17.24 ng/ml resulted in sensitivity of 61.4% and specificity of 91.7% for diagnosis of all types of pancreatic disease. The sensitivity rose to 65.85% and 78.26% for the diagnosis of all types of acute pancreatitis and severe acute pancreatitis, respectively. Serum cPE-1 is more sensitive at diagnosing severe acute pancreatitis than chronic or mild acute pancreatitis, and has a high positive likelihood ratio. Dogs with chronic pancreatitis tended to have lower serum cPE-1 concentration, suggesting decreased exocrine function.

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“…MDA content in serum can indicate the level of free radical overproduction [29,30] . In addition, a great quantity of endotoxin can induce TNF-α, stimulate or promote cytokine release including IL-1β [31] , IL-6, TNF, and further mediate activation of leucocyte and platelet in multiple organs, such as pancreas, kidney and lung, and release lysosome, oxygen-free radical and lipid inflammatory substance. The excessive cytokines and inflammatory factors can cause waterfall-like cascade reactions, induce iNOS expression all over the body, generate a great deal of NO, damage blood vessel endothelium, and cause tissue necrosis [32][33][34][35][36] .…”

Section: Discussionmentioning

confidence: 99%

Experimental study of therapeutic efficacy of Baicalin in rats with severe acute pancreatitis

Zhang

Zhang²,

He³

et al. 2007

WJG

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AIM:To observe the therapeutic efficacy of Baicalin in rats with severe acute pancreatitis (SAP) and explore its therapeutic mechanisms. METHODS:The SAP rat models were randomly divided into the model control group, Baicalin treatment group, octreotide treatment group and sham operation group. All groups were randomly subdivided into 3 h, 6 h and 12 h groups with 15 rats in each group. The survival, ascites volume and pathological changes of pancreas in all rats were observed at different time points after operation. The plasma amylase content and serum TNF-α, IL-6, malonaldehyde (MDA) and PLA2 contents were also determined. RESULTS:The survival was not obviously different between the treated groups, and was significantly higher in treated groups at 12 h compared to the model control group (P < 0.05, 15 vs 10). The ascites/body weight ratio at 3 h and 6 h was significantly lower in Baicalin treatment group compared to the model control group and octreotide treatment group (P < 0.05, 1.00 vs 2.02 and 1.43 and P < 0.001, 2.29 (1.21) vs 2.70 (0.80) and 2.08 (2.21), respectively). The contents of amylase, TNF-α, IL-6, MDA and PLA2 were significantly lower in the treated groups than in the model control group (P < 5.36 in IL-6, respectively). The pathological scores of pancreas in the treated groups were significantly lower than that in the model control group (P < 0.05, 9.00 vs 10.05, 6.00 vs 9.00, 8.00 vs 10.05), but no marked difference was found between the treated groups. CONCLUSION:The Baicalin injection has significant therapeutic effects on SAP rats, its effects are similar to those of octreotide. The Baicalin injection is also cheap and has a big application range, quite hopefully to be used in clinical treatment of SAP.

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Pancreatic proteases in serum induce leukocyte-endothelial adhesion and pancreatic microcirculatory failure

Cited by 37 publications

References 68 publications

Practice Guidelines in Acute Pancreatitis

Practice Guidelines in Acute Pancreatitis

Specificity and sensitivity of serum canine pancreatic elastase-1 concentration in the diagnosis of pancreatitis

Experimental study of therapeutic efficacy of Baicalin in rats with severe acute pancreatitis

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