Pancreatic metastasis from malignant phyllodes tumor of the breast

Lee, Seung Eun; Bae, Young Kyung; Choi, Jung-Hwa

doi:10.12701/yujm.2020.00759

Cited by 3 publications

(2 citation statements)

References 13 publications

(35 reference statements)

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“…Recurrence is positively associated with tumour malignancy, with benign, borderline and malignant recurrence rates of 6%–9%, 11%–16% and 14%–21%, respectively 5 . Metastases can occur in the lungs, bones, pleura and pancreas, which may have fatal effects on patients 6–8 . Furthermore, morphologically similar tumour cells can exhibit different behaviours, outcomes and responses to treatment.…”

Section: Introductionmentioning

confidence: 99%

“… 5 Metastases can occur in the lungs, bones, pleura and pancreas, which may have fatal effects on patients. 6 , 7 , 8 Furthermore, morphologically similar tumour cells can exhibit different behaviours, outcomes and responses to treatment. The lack of understanding of the inherent heterogeneity of breast PTs renders the assessment of malignant behaviour and growth trend of tumour cells in clinical practice difficult.…”

Section: Introductionmentioning

confidence: 99%

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Integrating single‐cell and spatial transcriptomes reveals COL4A1/2 facilitates the spatial organisation of stromal cells differentiation in breast phyllodes tumours

Li,

Yu,

et al. 2024

Clinical & Translational Med

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BackgroundBreast phyllodes tumours (PTs) are a unique type of fibroepithelial neoplasms with metastatic potential and recurrence tendency. However, the precise nature of heterogeneity in breast PTs remains poorly understood. This study aimed to elucidate the cell subpopulations composition and spatial structure and investigate diagnostic markers in the pathogenesis of PTs.MethodsWe applied single‐cell RNA sequencing and spatial transcriptomes on tumours and adjacent normal tissues for integration analysis. Immunofluorescence experiments were conducted to verify the tissue distribution of cells. Tumour cells from patients with PTs were cultured to validate the function of genes. To validate the heterogeneity, the epithelial and stromal components of tumour tissues were separated using laser capture microdissection, and microproteomics data were obtained using data‐independent acquisition mass spectrometry. The diagnostic value of genes was assessed using immunohistochemistry staining.ResultsTumour stromal cells harboured seven subpopulations. Among them, a population of widely distributed cancer‐associated fibroblast‐like stroma cells exhibited strong communications with epithelial progenitors which underwent a mesenchymal transition. We identified two stromal subpopulations sharing epithelial progenitors and mesenchymal markers. They were inferred to further differentiate into transcriptionally active stromal subpopulations continuously expressing COL4A1/2. The binding of COL4A1/2 with ITGA1/B1 facilitated a growth pattern from the stroma towards the surrounding glands. Furthermore, we found consistent transcriptional changes between intratumoural heterogeneity and inter‐patient heterogeneity by performing microproteomics studies on 30 samples from 11 PTs. The immunohistochemical assessment of 97 independent cohorts identified that COL4A1/2 and CSRP1 could aid in accurate diagnosis and grading.ConclusionsOur study demonstrates that COL4A1/2 shapes the spatial structure of stromal cell differentiation and has important clinical implications for accurate diagnosis of breast PTs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Integrating single‐cell and spatial transcriptomes reveals COL4A1/2 facilitates the spatial organisation of stromal cells differentiation in breast phyllodes tumours

Li,

Yu,

et al. 2024

Clinical & Translational Med

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Diagnostic approach to fibroepithelial tumors of the breast

Tresserra¹,

Martínez-Lanao²,

Fernandez-Acevedo³

et al. 2022

Revista de Senología y Patología Mamaria

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Case report: Osteosarcomatous differentiation in the lung metastasis of a malignant phyllodes tumor

Liu

Xue

et al. 2023

Front. Med.

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Malignant phyllodes tumor is a rare breast tumor, with distant metastases and heterologous differentiation in a few cases. We report a case of malignant phyllodes tumor with liposarcomatous differentiation in the primary tumor and osteosarcomatous differentiation in the lung metastatic tumor. A middle-aged female presented with a well-defined mass in the upper lobe of the right lung measuring 5.0 × 5.0 × 3.0 cm. The patient had a history of malignant phyllodes tumor in the breast. The patient underwent a right superior lobectomy. Histologically, the primary tumor was a typical malignant phyllodes tumor with pleomorphic liposarcomatous differentiation, while the lung metastasis showed osteosarcomatous differentiation without original biphasic features. The phyllodes tumor and heterologous components showed CD10 and p53 expression, and were negative for ER, PR, and CD34. Exome sequencing revealed TP53, TERT, EGFR, RARA, RB1, and GNAS mutations in all three components. Although the lung metastasis were morphologically different from the primary breast tumor, their common origin was demonstrated through immunohistochemical and molecular characterization. Cancer stem cells give rise to tumor heterogeneous cells, and heterologous components in malignant phyllodes tumors may indicate unfavorable prognosis and a greater risk of early recurrence and metastasis.

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Pancreatic metastasis from malignant phyllodes tumor of the breast

Cited by 3 publications

References 13 publications

Integrating single‐cell and spatial transcriptomes reveals COL4A1/2 facilitates the spatial organisation of stromal cells differentiation in breast phyllodes tumours

Integrating single‐cell and spatial transcriptomes reveals COL4A1/2 facilitates the spatial organisation of stromal cells differentiation in breast phyllodes tumours

Diagnostic approach to fibroepithelial tumors of the breast

Case report: Osteosarcomatous differentiation in the lung metastasis of a malignant phyllodes tumor

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