Pancreatic Islet Blood Flow Dynamics in Primates

Díez, Juan; Drigo, Rafael Arrojo e; Zheng, Xiaofeng; Stelmashenko, Olga; Chua, Minni; Rodriguez-Diaz, Rayner; Fukuda, Masahiro; Köhler, Martin; Leibiger, Ingo B.; Tun, Sai Bo Bo; Ali, Yusuf; Augustine, George J; Barathi, Veluchamy A; Berggren, Per Olof

doi:10.1016/j.celrep.2017.07.039

Cited by 37 publications

(39 citation statements)

References 35 publications

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“…Using an in vivo platform in non‐human primates, Diez et al transplanted islets autologously into the anterior chamber of the eye and monitored them non‐invasively and longitudinally at single‐cell resolution. Their investigation found that blood flow to islets is dynamic and is controlled by a vasoconstriction of the capillaries, potentially under the control of autonomic innervation . This study also demonstrated no changes in velocity with glucose or the GLP‐1 analog, liraglutide; in contrast to the findings of previous studies in the rodent pancreatic model …”

Section: Intravital Imaging Visualizes In Vivo Cellular Dynamics and contrasting

confidence: 60%

Intravital Imaging Techniques for Biomedical and Clinical Research

et al. 2019

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Intravital imaging, the direct visualization of cells and tissues within a living animal, is a technique that has been employed for the better part of a century. The advent of confocal and multiphoton microscopy has dramatically improved the power of intravital imaging, making it possible to obtain optical sections of tissues non-destructively. This review discusses the various techniques used for intravital imaging, describes how intravital imaging provides information about cellular and tissue dynamics not possible to be garnered by other techniques, and details several ways in which intravital imaging is making a direct impact on the clinical care of patients.

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Section: Intravital Imaging Visualizes In Vivo Cellular Dynamics and contrasting

confidence: 60%

Intravital Imaging Techniques for Biomedical and Clinical Research

et al. 2019

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“…Furthermore, the exposed location of the islets in the eye allows for manipulation by local treatment or, for that matter, light, and can illustrate, for example, the effects of parasympathetic innervation [ 23 , 54 ]. In addition, non-human primates can be used as both donors and acceptors for islet transplantation to the anterior chamber of the eye to study non-rodent islet physiology in vivo [ 71 ]. In vivo imaging of islet vasculature [ 71 – 73 ] and islet function [ 69 , 70 , 74 ] have been performed successfully in anaesthetised animals, specifically, the mouse [ 69 , 70 , 72 – 74 ] and non-human primate [ 71 ].…”

Section: How To Further Investigate Alpha Cell–beta Cell Interactionsmentioning

confidence: 99%

Alpha cell regulation of beta cell function

2020

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The islet of Langerhans is a complex endocrine micro-organ consisting of a multitude of endocrine and non-endocrine cell types. The two most abundant and prominent endocrine cell types, the beta and the alpha cells, are essential for the maintenance of blood glucose homeostasis. While the beta cell produces insulin, the only blood glucose-lowering hormone of the body, the alpha cell releases glucagon, which elevates blood glucose. Under physiological conditions, these two cell types affect each other in a paracrine manner. While the release products of the beta cell inhibit alpha cell function, the alpha cell releases factors that are stimulatory for beta cell function and increase glucose-stimulated insulin secretion. The aim of this review is to provide a comprehensive overview of recent research into the regulation of beta cell function by alpha cells, focusing on the effect of alpha cell-secreted factors, such as glucagon and acetylcholine. The consequences of differences in islet architecture between species on the interplay between alpha and beta cells is also discussed. Finally, we give a perspective on the possibility of using an in vivo imaging approach to study the interactions between human alpha and beta cells under in vivo conditions.

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“…Moreover, neurons and vascular supplies re-wire ( Rodriguez-Diaz et al . 2012 , Diez et al . 2017 ), affording the opportunity to investigate the influence of these on beta cell heterogeneity, and function/fate/plasticity can be followed longitudinally in the same animal (e.g.…”

Section: New Tools For Understanding Beta Cell Heterogeneitymentioning

confidence: 99%

The role of beta cell heterogeneity in islet function and insulin release

Nasteska

Hodson

2018

Journal of Molecular Endocrinology

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It is becoming increasingly apparent that not all insulin-secreting beta cells are equal. Subtle differences exist at the transcriptomic and protein expression levels, with repercussions for beta cell survival/proliferation, calcium signalling and insulin release. Notably, beta cell heterogeneity displays plasticity during development, metabolic stress and type 2 diabetes mellitus (T2DM). Thus, heterogeneity or lack thereof may be an important contributor to beta cell failure during T2DM in both rodents and humans. The present review will discuss the molecular and cellular features of beta cell heterogeneity at both the single-cell and islet level, explore how this influences islet function and insulin release and look into the alterations that may occur during obesity and T2DM.

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Pancreatic Islet Blood Flow Dynamics in Primates

Cited by 37 publications

References 35 publications

Intravital Imaging Techniques for Biomedical and Clinical Research

Intravital Imaging Techniques for Biomedical and Clinical Research

Alpha cell regulation of beta cell function

The role of beta cell heterogeneity in islet function and insulin release

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