Pancreatic Extract and the Intestinal Uptake of Vitamin B<sub>12</sub>

Lippe, G von der; Andersen, Knut-Jan; Schjønsby, H

doi:10.1080/00365521.1977.12031131

Cited by 10 publications

(3 citation statements)

References 19 publications

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“…Combinamide was given simultaneously with the isotopes in a dosage of 250 times (on a molar basis) the amount of cobalamin (9). Plasma-BIZ Plasma-By, (P-B 12 ) was measured by a radioisotope dilution assay (10).…”

Section: The Fab]2 Test Combined With Administration Of Epes or Cobin...mentioning

confidence: 99%

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The Role of the Exocrine Pancreas in Early‐Onset Vitamin B₁₂ Malabsorption in Gluten‐Challenged Celiac Children

Hjelt

1991

J. pediatr. gastroenterol. nutr.

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SummaryThe fractional absorption of vitamin B12 (FAB12) was measured by a double‐isotope technique specially adapted for children. In six celiac children on a strict gluten‐free diet and with a normal small intestinal biopsy, the FAB12 performed in the fasting patient averaged 30% (23–40%). After gluten challenge for a mean of 2 months (range 1–4), when mucosal damage was demonstrated by biopsy, the average fasting FAB12 in these patients decreased to 10% (0–17%) (p < 0.05). However, when the FAB12 test was repeated by means of stimulation by a B12‐free meal 1–3 weeks later, while the patients were still on a diet containing gluten, a significant increase was observed (mean 21%, range 14–27%) (p < 0.05). In four of the six patients the B12 absorption was further evaluated by repeating the FAB12 test by means of intravenous cholecystokinin (CCK) stimulation (n = 3) or by administration of exocrine pancreas enzyme supplementation (EPES) (n = 2) or cobinamide (n = 1). These tests all showed FAB12 values within the range of the meal‐stimulated FAB12. Moreover, in eight gluten‐free celiac children with normal biopsies, no difference was found between fasting and meal‐stimulated FAB12 values. Therefore, it is likely that the early‐onset B12 malabsorption observed in the gluten‐challenged celiac child with upper‐small‐intestinal mucosal damage is in part due to an insufficient stimulation of the exocrine pancreas when using the standard fasting B12 absorption test. These results indicate that a B12 absorption test with meal stimulation is preferable to the standard fasting test in children with small‐intestinal mucosal damage.

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Section: The Fab]2 Test Combined With Administration Of Epes or Cobin...mentioning

confidence: 99%

“…Therefore, the additional radiation dose due to FAB l2 tests other than the standard test was limited to 6-18 mrad (4). (3,9,11,12). Allen and colleagues (3) showed that human salivary HC (previously designated R protein) binds B 12 with greater affinity than human IF and that B 12 is primarily bound to HC.…”

Section: Radiation Dosementioning

confidence: 99%

The Role of the Exocrine Pancreas in Early‐Onset Vitamin B₁₂ Malabsorption in Gluten‐Challenged Celiac Children

Hjelt

1991

J. pediatr. gastroenterol. nutr.

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“…It has been proposed that pancreatic enzymes enhance the vitamin Biz absorption in an undefined manner by modifying crude sources of gastric juice (27). However, it is more possible that pancreatic enzymes enhance the absorption of IF-bound vitamin Biz indirectly by an effect on non-IF vitamin Biz binders in the intestinal juice (2,29).…”

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confidence: 99%

The Effect of Proteolytic Enzymes on the Vitamin B₁₂-binding Proteins of Human Gastric Juice and Saliva

Andersen

Lippe

1979

Scandinavian Journal of Gastroenterology

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Pepsin had no effect on the vitamin B12 binder in human saliva (R-binder), while trypsin was found to reduce the apparent molecular weight of the R-binder and to release vitamin B12 from the R-B12complex of human saliva and human gastric juice (HGJ). Trypsin had no effect on the molecular weight and biological activity of intrinsic factor (IF) in HGJ, as demonstrated by gel filtration on Sephadex G-150 and the uptake of IF-B12 by guinea pig intestinal brush borders. An extract of purified guinea pig intestinal lysosomes was also without effect on the molecular weight and the biological activity of IF but was found to release vitamin B12 from the R-B12 complex. The results support the observation that the external pancreatic secretion corrects malabsorption of vitamin B12 by an effect on the non-IF protein in the intestinal juice. Moreover, the results indicate that lysosomal enzymes are not involved in the intestinal absorption of vitamin B12.

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Gastric Intrinsic Factor and Cobalamin Absorption

Seetharam

Alpers

1991

Comprehensive Physiology

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Pancreatic Extract and the Intestinal Uptake of Vitamin B₁₂

Cited by 10 publications

References 19 publications

The Role of the Exocrine Pancreas in Early‐Onset Vitamin B₁₂ Malabsorption in Gluten‐Challenged Celiac Children

The Role of the Exocrine Pancreas in Early‐Onset Vitamin B₁₂ Malabsorption in Gluten‐Challenged Celiac Children

The Effect of Proteolytic Enzymes on the Vitamin B₁₂-binding Proteins of Human Gastric Juice and Saliva

Gastric Intrinsic Factor and Cobalamin Absorption

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Pancreatic Extract and the Intestinal Uptake of Vitamin B12

Cited by 10 publications

References 19 publications

The Role of the Exocrine Pancreas in Early‐Onset Vitamin B12 Malabsorption in Gluten‐Challenged Celiac Children

The Role of the Exocrine Pancreas in Early‐Onset Vitamin B12 Malabsorption in Gluten‐Challenged Celiac Children

The Effect of Proteolytic Enzymes on the Vitamin B12-binding Proteins of Human Gastric Juice and Saliva

Gastric Intrinsic Factor and Cobalamin Absorption

Contact Info

Product

Resources

About

Pancreatic Extract and the Intestinal Uptake of Vitamin B₁₂

The Role of the Exocrine Pancreas in Early‐Onset Vitamin B₁₂ Malabsorption in Gluten‐Challenged Celiac Children

The Role of the Exocrine Pancreas in Early‐Onset Vitamin B₁₂ Malabsorption in Gluten‐Challenged Celiac Children

The Effect of Proteolytic Enzymes on the Vitamin B₁₂-binding Proteins of Human Gastric Juice and Saliva