Pancreatic Exocrine Function in Neoplastic and Inflammatory Disease; A Simple and Reliable New Test

Lundh, G

doi:10.1016/s0016-5085(62)80025-0

Cited by 225 publications

(50 citation statements)

References 7 publications

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“…Consideration was given to the presence of etiologic factors such as biliary tract disease or chronic alcoholism. Symptoms and signs of abdominal pain, jaundice, steatorrhea, diabetes mellitus or gastrointestinal ANGlOGRAPHIC DIAGNOSIS OF INFLAMMATORY DISEASE bleeding were also considered as well as laboratory tests, including the analysis of serum or urinary amylase, estimation of trypsin in the duodenal content (LUNDH 1962), determination of faecal fat and tests of glucose metabolism. The operation and pathology reports were also evaluated as well as the duration of the condition.…”

Section: Methodsmentioning

confidence: 99%

Angiographic Diagnosis of Inflammatory Disease of the Pancreas

Tylén

Arnesjö

1973

Acta Radiologica. Diagnosis

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Section: Methodsmentioning

confidence: 99%

Angiographic Diagnosis of Inflammatory Disease of the Pancreas

Tylén

Arnesjö

1973

Acta Radiologica. Diagnosis

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“…The levels of pancreatic lipolytic enzymes in human small-intestinal contents are generally determined by measuring enzyme activities (1)(2)(3)(4). In recent years radioimmunoassays for the pancreatic lipolytic enzymes have also become available (5,6).…”

Section: B Sternby Dept Of Medical and Physiological Chemistry 4 mentioning

confidence: 99%

Pancreatic Lipolytic Enzymes in Human Duodenal Contents Radioimmunoassay Compared with Enzyme Activity

Sternby

Nilsson

Melin

et al. 1991

Scandinavian Journal of Gastroenterology

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The total pancreatic lipolytic capacity was determined in duodenal contents in healthy humans 10-120 min after a liquid test meal, by estimating the amount of pancreatic lipase, colipase, carboxyl ester lipase, and phospholipase A2 by means of radioimmunoassays and enzymatic assays. The molar concentrations of the different proteins were of the same order of magnitude. The relative specific activity (enzyme activity/milligram immunoreactive protein expressed as a percentage of the specific activity of the respective pure protein) amounted to 75-120% for lipase, 45-80% for colipase, 30-70% for carboxyl ester lipase, and 45-120% for phospholipase A2. These varied, and sometimes low values can be explained by the fact that the enzymes are inhibited or partly inactivated in the duodenal contents by surface denaturation, in which cases the products are still immunoreactive. Also, the proforms of colipase and phospholipase A2 may not always be completely activated. Furthermore, the specific activities of the pure enzymes (and thus the relative specific activities) are related to the methods used, which are not specific enough to distinguish completely the three enzymes and the cofactor in duodenal contents.

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“…Indirect stimulation of the pancreas by means (2) has been found to be a valuable test of pancreatic function and digestive capacity in man (14, 16). We found it suitable for evaluation of the ability of pancreatic replacement preparations to affect the enzyme concentrations in the upper part of the small intestine.…”

Section: Discussionmentioning

confidence: 99%

Intestinal Concentrations of Pancreatic Enzymes following Pancreatic Replacement Therapy

Ihse

Lilja

Lundquist

1980

Scandinavian Journal of Gastroenterology

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The activities of amylase, lipase, phospholipase, and trypsin in four commercially available preparations of pancreatin with different galenic and adjunctive protective properties were estimated in vitro, using human small-intestinal juice as the incubation medium. These preparations were administered to healthy subjects and to patients with severe pancreatic insufficiency, and their ability to increase the intestinal concentrations of pancreatic enzymes was evaluated. The relations between in vitro and in vivo activities were also studied. In vitro testing showed that the preparations contained high but varying activities of enzymes, with the greatest variations in lipase and trypsin. Pancreatin in the form of tablets, with or without protective measures against acid, did not cause any apparent increase in the activities of pancreatic enzymes in the upper part of the gut in patients with pancreatic insufficiency. Granulated pancreatin, on the other hand, brought about an increase in the activities of amylase, phospholipase, lipase, and trypsin. Relatively higher activities of the enzymes in granulated form reached the small intestine as compared with those of the tablets.

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Pancreatic Exocrine Function in Neoplastic and Inflammatory Disease; A Simple and Reliable New Test

Cited by 225 publications

References 7 publications

Angiographic Diagnosis of Inflammatory Disease of the Pancreas

Angiographic Diagnosis of Inflammatory Disease of the Pancreas

Pancreatic Lipolytic Enzymes in Human Duodenal Contents Radioimmunoassay Compared with Enzyme Activity

Intestinal Concentrations of Pancreatic Enzymes following Pancreatic Replacement Therapy

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