Pancreatic cancer gene therapy using an siRNA-functionalized single walled carbon nanotubes (SWNTs) nanoplex

Anderson, Tommy; Hu, Rui; Yang, Chengbin; Yoon, Ho Sup; Yong, Ken‐Tye

doi:10.1039/c4bm00019f

Cited by 40 publications

(21 citation statements)

References 73 publications

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“…The efficacy of SWCNT/siCCR5 formulation was evaluated by assessing the CCR5 siRNA-mediated knockdown in HepG2 cells after 48 h transfection via flow cytometry, as CCR5 is known to be expressed in HepG2 cells [70]. SWCNTs-formulated siRNA transfection showed much lower expression of chemokine receptor type 5 (CCR5) as compared to the natural expression exhibited in the control sample (Figure 7), indicating substantial (over 90%) knockdown above or comparable to that regularly achieved by lyposomally delivered siRNA [18,71,72].…”

Section: Resultsmentioning

confidence: 98%

Multi-Drug/Gene NASH Therapy Delivery and Selective Hyperspectral NIR Imaging Using Chirality-Sorted Single-Walled Carbon Nanotubes

Hasan

Campbell

Сизова

et al. 2019

Cancers

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Single-walled carbon nanotubes (SWCNTs) can serve as drug delivery/biological imaging agents, as they exhibit intrinsic fluorescence in the near-infrared, allowing for deeper tissue imaging while providing therapeutic transport. In this work, CoMoCAT (Cobalt Molybdenum Catalyst) SWCNTs, chirality-sorted by aqueous two-phase extraction, are utilized for the first time to deliver a drug/gene combination therapy and image each therapeutic component separately via chirality-specific SWCNT fluorescence. Each of (7,5) and (7,6) sorted SWCNTs were non-covalently loaded with their specific payload: the PI3 kinase inhibitor targeting liver fibrosis or CCR5 siRNA targeting inflammatory pathways with the goal of addressing these processes in nonalcoholic steatohepatitis (NASH), ultimately to prevent its progression to hepatocellular carcinoma. PX-866-(7,5) SWCNTs and siRNA-(7,6) SWCNTs were each imaged via characteristic SWCNT emission at 1024/1120 nm in HepG2 and HeLa cells by hyperspectral fluorescence microscopy. Wavelength-resolved imaging verified the intracellular transport of each SWCNT chirality and drug release. The therapeutic efficacy of each formulation was further demonstrated by the dose-dependent cytotoxicity of SWCNT-bound PX-866 and >90% knockdown of CCR5 expression with SWCNT/siRNA transfection. This study verifies the feasibility of utilizing chirality-sorted SWCNTs for the delivery and component-specific imaging of combination therapies, also suggesting a novel nanotherapeutic approach for addressing the progressions of NASH to hepatocellular carcinoma.

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Section: Resultsmentioning

confidence: 98%

Multi-Drug/Gene NASH Therapy Delivery and Selective Hyperspectral NIR Imaging Using Chirality-Sorted Single-Walled Carbon Nanotubes

Hasan

Campbell

Сизова

et al. 2019

Cancers

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“…[62] Chen et al have showed that the mouse double minute 2 homolog (MDM2) gene inhibiting small interfering RNA (siRNA) improves the efficiency of gene silencing to 83.55 % when coupled with amino-functionalized SWCNTs. [63] Similarly, Anderson et al [64] have utilized fluorescent microscopy and flow cytometry to achieve high transfection efficiency of phospholipid polyethylene glycol (PL-PEG) coated SWCNTs-siRNA complex into the pancreatic cells. The release of siRNA from the CNT-complex intracellularly ensured targeting of the mutant K-Ras gene in PANC-1 cancer cells.…”

Section: Cancer Therapymentioning

confidence: 99%

Derivatized Carbon Nanotubes for Gene Therapy in Mammalian and Plant Cells

et al. 2020

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The concept of gene vectors for therapeutic applications has been known for several years, but it is far from revealing its actual potential. With the advent of hollow cylindrical carbon nanomaterials such as carbon nanotubes (CNTs), researchers have invented several new tools to deliver genes at the required site of action in mammalian and plant cells. The ease of diversified functionalization has allowed CNTs to be by far the most adaptable non‐viral vector for gene therapy. This Minireview addresses the dexterity with which CNTs undergo surface modifications and their applications as a potent vector in gene therapy of humans and plants. Specifically, we will discuss the new tools that scientific communities have invented to achieve gene therapy using plasmid DNA, RNA silencing, suicide gene therapy, and plant genetic engineering. Additionally, we will shed some light on the mechanism of gene transportation using carbon nanotubes in cancer cells and plants.

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“…181 Anderson et al fabricated PEGylated SWCNTs, functionalized them with poly(allylamine hydrochloride), and adsorbed siRNAs on them to produce a CNT-based transfection agent. 182 Similarly, Siu et al has modified SWCNTs with lipid-modified poly(ethyleneimine) for successful siRNA delivery both in vitro and in vivo. 183 Weaver et al proposed a novel polypyrrole-GO-dexamethasone complex that can release the anti-inflammatory drug dexamethasone upon electrical stimulation.…”

Section: Drug Deliverymentioning

confidence: 99%

Recent advances in bioactive 1D and 2D carbon nanomaterials for biomedical applications

Erol

Uyan

Hatip

et al. 2018

Nanomedicine: Nanotechnology, Biology and Medicine

117

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One-dimensional (1D) carbon nanotubes (CNTs) and the two-dimensional (2D) graphene represent the most widely studied allotropes of carbon. Due to their unique structural, electrical, mechanical and optical properties, 1D and 2D carbon nanostructures are considered to be leading candidates for numerous applications in biomedical fields, including tissue engineering, drug delivery, bioimaging and biosensors. The biocompatibility and toxicity issues associated with these nanostructures have been a critical impediment for their use in biomedical applications. In this review, we present an overview of the various materials types, properties, functionalization strategies and characterization methods of 1D and 2D carbon nanomaterials and their derivatives in terms of their biomedical applications. In addition, we discuss various factors and mechanisms affecting their toxicity and biocompatibility.

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Pancreatic cancer gene therapy using an siRNA-functionalized single walled carbon nanotubes (SWNTs) nanoplex

Cited by 40 publications

References 73 publications

Multi-Drug/Gene NASH Therapy Delivery and Selective Hyperspectral NIR Imaging Using Chirality-Sorted Single-Walled Carbon Nanotubes

Multi-Drug/Gene NASH Therapy Delivery and Selective Hyperspectral NIR Imaging Using Chirality-Sorted Single-Walled Carbon Nanotubes

Derivatized Carbon Nanotubes for Gene Therapy in Mammalian and Plant Cells

Recent advances in bioactive 1D and 2D carbon nanomaterials for biomedical applications

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