Pancreatic and bile duct obstruction exacerbates rat caerulein-induced pancreatitis: a new experimental model of acute hemorrhagic pancreatitis

Yamasaki, Mitsuo; Takeyama, Yoshifumi; Shinkai, Masayuki; Ohyanagi, Harumasa

doi:10.1007/s00535-005-1767-9

Cited by 8 publications

(2 citation statements)

References 15 publications

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“…In previous reports, caerulein (an analogue of the human gastrointestinal hormone cholecystokinin) was used to stimulate smooth muscle contraction and release of biliary and pancreatic enzymes. At high concentrations, caerulein is reported to stimulate the inflammation, acinar loss, and fibrosis typically found in pancreatitis 13,14 . We therefore adopted caerulein treatment (70 μg/kg) as a positive control.…”

Section: Resultsmentioning

confidence: 99%

Long-term Proton Pump Inhibitor Administration Caused Physiological and Microbiota Changes in Rats

Yang

Chang

Lin

et al. 2020

Sci Rep

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proton pump inhibitors (ppis) are used for the long-term treatment of gastroesophageal disorders and the non-prescription medicines for acid reflux. However, there is growing concerns about PPI misuse, overuse and abuse. This study aimed to develop an animal model to examine the effects of long-term use of ppi in vivo. Twenty one Wistar rats were given omeprazole orally or intravenously for 30 days, and caerulein as a positive control. After euthanization, the serum and stool were collected to perform MS-based quantitative analysis of metabolites. We carried out 16S-based profiling of fecal microbiota, assessed the expression of bile acid metabolism regulators and examined the immunopathological characteristics of bile ducts. After long-term PPI exposure, the fecal microbial profile was altered and showed similarity to those observed in high-fat diet studies. The concentrations of several metabolites were also changed in various specimens. Surprisingly, morphological changes were observed in the bile duct, including ductal epithelial proliferation, micropapillary growth of biliary epithelium, focal bile duct stricture formation and bile duct obstruction. these are characteristics of precancerous lesions of bile duct. fXR and RXRα expressions were significantly reduced, which were similar to that observed in cholangiocarcinoma in TCGA and Oncomine databases. We established a novel animal model to examine the effects of long-term use of omeprazole. The gut microbes and metabolic change are consequences of long-term PPI exposure. And the results showed the environment in vivo tends to a high-fat diet. More importantly, we observed biliary epithelial hyperplasia, which is an indicator of a high-fat diet. Proton Pump Inhibitors (PPIs) are one of the most prescribed drugs in many countries. It inhibits the H + /K + ATPase enzyme system in the stomach to suppress acid production, resulting in higher gastric pH. Long-term PPI use is an effective treatment for gastroesophageal disorders and Helicobacter pylori infection, and are taken without prescription to relieve heartburn 1. Despite their increased usage and popularity, over-the-counter (OTC) PPIs such as omeprazole, lansoprazole and pantoprazole are prone to overuse and misuse. There are growing concerns in the medical community about the inappropriate and overuse of PPIs 2-4 .

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Section: Resultsmentioning

confidence: 99%

Long-term Proton Pump Inhibitor Administration Caused Physiological and Microbiota Changes in Rats

Yang

Chang

Lin

et al. 2020

Sci Rep

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“…However, the mouse pancreas has several pancreatic ducts that differ significantly -this feature makes complete obstruction of the pancreatic ducts difficult, but at the same time provides internal control, since it is easy to obstruct only a subset of pancreatic segments. Obstruction of only one duct in rats practically does not lead to pancreatitis, but in combination with mild stimulation with secretogens causes acute pancreatitis [1][2][3]. Such stimulation can be used to simulate chronic pancreatitis [4].…”

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confidence: 99%

Models of Chronic Pancreatitis

BENASHVILI

Topuria

MATOSHVILI

et al. 2023

jecm

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departments: 1 Clinical Anatomy and Operative Surgery; 2 Human Normal Anatomy, 3 Dermatology and Venereology, 4 Clinical Skills SUMMARY Laboratory models of acute and chronic pancreatitis in animals are created in order to study the mechanisms of pathogenesis, develop new methods of treatment and carcinogenesis during inflammation of the pancreas. Both in vitro models are used to study the early stage, short-term processes in which acinar cells are involved, as well as models that cause the development of mild or severe forms of the disease in rodents.Although rodents are most commonly used in models of pancreatitis, the pancreatic damage they cause does not necessarily fully correspond to human pathology. Therefore, it is necessary to carefully choose the model most suitable for the answer in this particular task. The purpose of this article is to compare the most widely used animal models of pancreatitis.

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Animal Modeling of Pancreatitis-to-Cancer Progression

Martinelli¹,

Real²

2017

Pancreatic Cancer

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Pancreatic and bile duct obstruction exacerbates rat caerulein-induced pancreatitis: a new experimental model of acute hemorrhagic pancreatitis

Abstract: Preceding biliopancreatic duct obstruction exacerbates caerulein-induced pancreatitis. Enhancement of intracellular trypsin activation is possibly involved in this mechanism.

Cited by 8 publications

References 15 publications

Long-term Proton Pump Inhibitor Administration Caused Physiological and Microbiota Changes in Rats

Long-term Proton Pump Inhibitor Administration Caused Physiological and Microbiota Changes in Rats

Models of Chronic Pancreatitis

Animal Modeling of Pancreatitis-to-Cancer Progression

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