Pancreas preservation by the 2-layer cold storage method before islet isolation protects isolated islets against apoptosis through the mitochondrial pathway

Augmented damage of islets by impaired exocrine acinar cells undergoing apoptosis that is possibly converted to necrosis during isolation

“…This suggested that apoptotic or necrotic cell death of islets might proceed during pancreas storage, which resulted in a decrease of the islet yield, viability and graft survival after transplantation. 5 Islet damage attributed to impaired exocrine cells during pancreas preservation and isolation procedure remains elusive, although released exocrine enzymes could directly damage islets. The aim of this study is to investigate the cellular mechanisms associated with exocrine cells and their possible impact on the islet cell survival and function after isolation.…”

“…2 Several studies observed reduction in isolated islets yield and function after cold preservation, which is increased by time of preservation. [3][4][5] In clinical settings, pancreas procurement and preservation before islet isolation are essential in islet allotransplant. This suggested that apoptotic or necrotic cell death of islets might proceed during pancreas storage, which resulted in a decrease of the islet yield, viability and graft survival after transplantation.…”

Pancreas Preservation for Pancreas and Islet Transplantation: A Minireview

“…Based on several studies, 38 -40 TLM has been widely used by islet transplant centers worldwide, but the mechanisms by which it improves human islet yield and quality are not yet fully understood. It has been suggested by Matsuda et al 41 that TLM cold storage protects isolated islets against apoptosis through the mitochondrial pathway. Noguchi et al, 42 with a pig model, reported that the islet yield from pancreata preserved with TLM and a modified so-called classic solution was significantly higher than with TLM using UW.…”

Chemical Oxygenation of Pancreatic Tissue Prior to Islet Isolation and Transplantation

“…Using this procedure the small intestine retrieved from rats , Tsujimura et al, 2004b or dogs (Tsujimura et al, 2002b, Fujino et al, 2003 could be successfully preserved for 24 to 48 hours. In addition, this technique provided also excellent survival rates of 80% after heterotopic transplantation of UW-perfused rat hearts after cold storage for 48 hours in oxygenated PFD (Kuroda et al, 1995).…”

“…One explanation for this phenomenon is that TLM re-initiates production of ATP in mitochondrial pathways by providing sufficient oxygen levels. One can speculate that maintaining the metabolic activity of mitochondria within a certain range seems to be efficient to prevent excessive generation of ROS in mitochondria as main subcellular producers of ROS (Li et al, 2008, Tsujimura et al, 2004a and to inhibit mitochondrial pathways of apoptotic cell death (Matsuda et al, 2003).…”