Pancreas Oxygen Persufflation Increases ATP Levels as Shown by Nuclear Magnetic Resonance

Scott, William E.; Weegman, Bradley P.; Ferrer-Fàbrega, Joana; Stein, Sandra; Anazawa, Takayuki; Kirchner, Varvara A.; Rizzari, Michael; Stone, J. J.; Matsumoto, Shuichiro; Hammer, Bruce E.; Balamurugan, Appakalai N.; Kidder, Louis S.; Suszynski, Thomas M.; Avgoustiniatos, Efstathios S.; Stone, Simon G.; Tempelman, Linda A.; Sutherland, David E.R.; Hering, Bernhard J.; Papas, Klearchos K.

doi:10.1016/j.transproceed.2010.05.091

Cited by 48 publications

(36 citation statements)

References 33 publications

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“…Our research group is currently studying PSF of the pancreas to parallel our concurrent interests in pancreas HMP [89; 91] and have recently published several works on A-PSF of porcine pancreata for the purposes of improving whole organ and islet quality [78; 79]. In one study [79], human and porcine pancreata were preserved using TLM or A-PSF at 4°C. A-PSF was performed via the superior mesenteric artery and either the splenic artery (human) or the celiac trunk (pig).…”

Section: Whole Organ Persufflationmentioning

confidence: 99%

Persufflation (or gaseous oxygen perfusion) as a method of organ preservation

et al. 2012

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Improved preservation techniques have the potential to improve transplant outcomes by better maintaining donor organ quality and by making more organs available for allotransplantation. Persufflation, (PSF, gaseous oxygen perfusion) is potentially one such technique that has been studied for over a century in a variety of tissues, but has yet to gain wide acceptance for a number of reasons. A principal barrier is the perception that ex vivo PSF will cause in vivo embolization post-transplant. This review summarizes the extensive published work on heart, liver, kidney, small intestine and pancreas PSF, discusses the differences between anterograde and retrograde PSF and between PSF and other conventional methods of organ preservation (static cold storage, hypothermic machine perfusion). Prospective implications of PSF within the broader field of organ transplantation, and in the specific application with pancreatic islet isolation and transplant are also discussed. Finally, key issues that need to be addressed before PSF becomes a more widely utilized preservation strategy are summarized and discussed.

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Section: Whole Organ Persufflationmentioning

confidence: 99%

Persufflation (or gaseous oxygen perfusion) as a method of organ preservation

et al. 2012

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“…Many factors contribute to islet yield and viability including donor related parameters, pancreas procurement and preservation methods [8–12], and especially the islet isolation process itself. Many islets are never retrieved during isolation [13] or are irreversibly damaged along the way [14–16].…”

Section: Introductionmentioning

confidence: 99%

Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability

Weegman

Sajja

Suszynski

et al. 2016

Journal of Diabetes Research

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Islet transplantation (ITx) is an emerging and promising therapy for patients with uncontrolled type 1 diabetes. The islet isolation and purification processes require exposure to extended cold ischemia, warm-enzymatic digestion, mechanical agitation, and use of damaging chemicals for density gradient separation (DG), all of which reduce viable islet yield. In this paper, we describe initial proof-of-concept studies exploring quadrupole magnetic separation (QMS) of islets as an alternative to DG to reduce exposure to these harsh conditions. Three porcine pancreata were split into two parts, the splenic lobe (SPL) and the combined connecting/duodenal lobes (CDL), for paired digestions and purifications. Islets in the SPL were preferentially labeled using magnetic microparticles (MMPs) that lodge within the islet microvasculature when infused into the pancreas and were continuously separated from the exocrine tissue by QMS during the collection phase of the digestion process. Unlabeled islets from the CDL were purified by conventional DG. Islets purified by QMS exhibited significantly improved viability (measured by oxygen consumption rate per DNA, p < 0.03) and better morphology relative to control islets. Islet purification by QMS can reduce the detrimental effects of prolonged exposure to toxic enzymes and density gradient solutions and substantially improve islet viability after isolation.

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“…In most studies, shorter cold ischaemic time associates with better islet yields (White et al 2001). Transport using a two-layer method where the pancreas rests on a substrate in the top layer of a perfluorocarbon ''bath'' appears to give slightly improved outcomes (Scott et al 2010a) and showed promising results in pancreata from humans (Scott et al 2010b). …”

Section: Pancreas Perfusion and Transport Factorsmentioning

confidence: 99%

Islet Transplantation: Factors in Short-Term Islet Survival

Deters

Stokes

Gunton

2011

Arch. Immunol. Ther. Exp.

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Islet transplantation has the potential to cure type 1 diabetes. In recent years, the proportion of patients achieving initial insulin independence has improved, but longer term outcomes remain poor compared to those for whole pancreas transplants. This review article will discuss factors affecting islet yield and viability leading up to transplantation and in the immediate post-transplant period.

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Pancreas Oxygen Persufflation Increases ATP Levels as Shown by Nuclear Magnetic Resonance

Cited by 48 publications

References 33 publications

Persufflation (or gaseous oxygen perfusion) as a method of organ preservation

Persufflation (or gaseous oxygen perfusion) as a method of organ preservation

Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability

Islet Transplantation: Factors in Short-Term Islet Survival

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