2017
DOI: 10.1038/nmeth.4429
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Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish

Abstract: Calcium imaging with cellular resolution typically requires an animal to be tethered under a microscope, which substantially restricts the range of behaviors that can be studied. To expand the behavioral repertoire amenable to imaging, we have developed a tracking microscope that enables whole-brain calcium imaging with cellular resolution in freely swimming larval zebrafish. This microscope uses infrared imaging to track a target animal in a behavior arena. On the basis of the predicted trajectory of the anim… Show more

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Cited by 199 publications
(205 citation statements)
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“…The high volumetric imaging speed and epi-illumination configuration together make LFM a well-suited technique to image freely moving animals, which are of great interests in neuroscience because of the extended behavioral paradigms to understand various complex brain functions 19,[36][37][38][39] . In particular, neural activity in groups of neurons during larval zebrafish's prey capture behavior, which is under extensive investigations [40][41][42][43] , has been recorded at relatively low spatial or temporal resolutions 31,44,45 .…”
Section: Whole Brain Functional Imaging Of Neural Activities In Freelmentioning
confidence: 99%
“…The high volumetric imaging speed and epi-illumination configuration together make LFM a well-suited technique to image freely moving animals, which are of great interests in neuroscience because of the extended behavioral paradigms to understand various complex brain functions 19,[36][37][38][39] . In particular, neural activity in groups of neurons during larval zebrafish's prey capture behavior, which is under extensive investigations [40][41][42][43] , has been recorded at relatively low spatial or temporal resolutions 31,44,45 .…”
Section: Whole Brain Functional Imaging Of Neural Activities In Freelmentioning
confidence: 99%
“…Zebrafish have also been used to study other more complex behaviors such as sleep/wake (Barlow & Rihel, 2017), learning and memory (Gerlai, 2017), locomotion (Müller & van Leeuwen, 2004), and affective depressive-like behaviors and roles of antidepressants (Ziv et al, 2013). Pairing behavioral work with direct imaging of neuronal excitability has recently begun to illuminate the activity of neuronal circuits in mediating the zebrafish behavioral repertoire (Cong et al, 2017;Kim et al, 2017). Pairing behavioral work with direct imaging of neuronal excitability has recently begun to illuminate the activity of neuronal circuits in mediating the zebrafish behavioral repertoire (Cong et al, 2017;Kim et al, 2017).…”
Section: Zebrafish As a Behavioral Modelmentioning
confidence: 99%
“…Current Protocols Essential Laboratory Techniques systems; Pan et al, 2013;Xiong, Obholzer, Noche, & Megason, 2015) will greatly facilitate the ability to describe the full connectome of the zebrafish brain. Similarly, the ability to genetically encode voltage or calcium sensors in distinct neurons or throughout the brain opens tremendous possibilities for recording and manipulating patterns of activity in well-defined circuits or across the whole CNS (Kibat et al, 2016;Kim et al, 2017;Naumann et al, 2016).…”
Section: Of 26mentioning
confidence: 99%
“…In terms of behaviour larvae exhibit an alternating 23 sequence of movements and pauses, termed bouts. This structure is particularly suited to modular 24 description as individual bouts can be easily segmented and it is relatively easy to acquire many 25 examples from even a single animal due to the high frequency of their movement (Kim et al, 2017). 26…”
Section: Introductionmentioning
confidence: 99%